Table of Content

28 February 2015, Volume 32 Issue 01

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Establishment and Preliminary Application of PCR Method for Detection of Feline Panleukopenia Virus

2015, 32(01):  1-6. 

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Abstract:Objective To establish a PCR method for detection of Feline panleukopenia virus ( FPV) in cats． Method The primers were designed and synthesized according to the published FPV specific sequences of VP2 gene． PCR method was established，and the specificity，sensitivity and stability of this method were tested． Then the method was used to detect 33 clinical samples of cats． Result The developed PCR method was no cross reaction with Feline herpes virus 1 ( FHV-1 )，Feline coronavirus ( FeCV)，Feline syncytium-forming virus (FeSFV)，Feline immunodefiency virus(FIV)． Its minimum detection limit (the lowest detection virus titer) was 5lgTCID50 /mL，the corresponding template DNA concentration was 4. 9 x 102 copies． FPV DNA，maintained at － 30 ℃ for 12 months，was still able to amplify the fragment of about 301 bp． The 33 cats were 21 positive ，and 11 negative for FPV detected by PCR． Conclusion The developed PCR method is good in specificity，sensitivity， stability，and can be used for detecting the FPV in cat．

Establishment and Evaluation of a Spinal Cord Injury Model with Bilateral Hemi-sections at Different Planes in Rats

2015, 32(01):  7-11. 

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Abstract:Objective To establish a novel rat spinal cord injury model so as to furnish a suitable animal model for clinical research of bilateral spinal cord injury at different planes． Method Forty-five Sprague-Dawley rats were randomly divided into bilateral spinal cord hemi-section group ( group A，n = 15)，spinal cord transection group (group B，n = 15 ) and sham-operation group ( group C，n = 15 )． After operation，the activities，ingesta， excretion，wound infection，death and other complications of rats in different groups were observed． Besides，BBB locomotive function scores and inclined plane test were performed to evaluate rats’behavioral function． Result After operation，the activities and ingesta were decreased in group A． Meanwhile，53. 33% of rats suffered from the uroschesis and 40. 00% died in the course of 28 － days’evaluation． In group B，The activities and injesta were obviously decreased，66. 67% of rats suffered from the uroschesis and 60. 00% died in 4 weeks post-injury． In group C，whereas，rats’activities and ingsta did not decrease as well as no complications and death occurred． The mean scores of BBB and maximum angle of inclined plane in group A and B were significantly lower than that in group C (P ＜ 0. 05)，but these parameters had no remarkable difference between group A and group B． Conclusion The spinal cord injury model with bilateral hemi-sections at different levels possesses the distinctions of lower incidence of uroschesis and mortality，and has not significant difference compared with the traditional spinal cord trans-ection model in behavioral evaluation． Thus，it is a suitable model for clinical research on bilateral spinal cord injury at different levels．

Effects and Underlying Mechanisms of Apelin-13 Injected by Intarcerebroventricular on Cerebral Ischemia-reperfusion Injury in Rat

2015, 32(01):  12-16. 

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Abstract: Objective To investigate the effects and underlying mechanisms of Apelin-13 injected by intarcerebroventricular on cerebral ischemia-reperfusion injury in middle cerebral artery occlusive (MCAO) rats． Method Focal cerebral ischemia in rats was established by 2 h occlusion of the middle cerebral artery and 24 h reperfusion． After 2h ischemia，Apelin-13 and NS were administrated intracerebroventricularly． The neurologic deficient scores were investigated according to Zea-Longa’s Standard． The infarct areas were assessed with software Imagepro Plus 6. 0 after TTC staining． The activities of inducible nitric oxide synthase ( iNOS) and glutathione peroxidase(GSH-PX)were determined by specific kit． And the levels of Bcl-2、Bax and Caspase-3 in central neutral system were measured by western blot． Result Apelin-13(1. 0 μg·kg － 1 ) could decrease the neurologic deficient score，reduce the infarct areas of the brain in CIRI rats，depressed the activity of iNOS，enhance the activity of GSH-PX，increase the expression of Bcl-2，and decrease the expression of Bax and Caspase-3． Conclusion Apelin-13 can protect the ischemia-reperfusion injury，the mechanism may be related to depressing the level of NO， getting rid of more ROS，adjusting the expression of apoptotic and anti-apoptotic proteins．

The Protective Effects of Bifendate and Oleanolic acid on Acute Hepatic Injury Induced by ANIT in Mice and Rat

2015, 32(01):  17-24. 

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Abstract:Objective To observe the protective effects of Bifendate and Oleanolic acid on acute hepatic injury induced by ANIT in olive oil in mice． Method At first，Bifendate and Oleanolic acid were taken by mice and rats． And then 100 mg /kg and 80 mg /kg ANIT were injected into abdominal cavity of mice and rats to damage their liver，respectively． After 48 hours，their fasting serum levels of ALT，AST，TBIL，DBIL，GGT，ALP were measured． The animals were sacrificed and dissected，and the wet weight of their liver and spleen as well were measured． Finally the viscera index was calculated． Result Oleanolic acid significantly reduced the serum ALT， AST and TB in mice，but Bifendate had no liver protection capacity． Bifendate significantly reduced the serum ALT，AST and ALP in rat，but Oleanolic acid had no liver protection effect． Conclusion Oleanolic acid has liver protection effect on liver injury induced by ANIT in olive oil in mice，and Bifendate has liver protection effect on liver injury induced by ANIT in olive oil in rat．

The Protective Effects of Bifendate and Oleanolic acid on Acute Hepatic Injury Induced by ANIT in Mice and Rat

2015, 32(01):  20-24. 

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Abstract:Objective To observe the protective effects of Bifendate and Oleanolic acid on acute hepatic injury induced by ANIT in olive oil in mice． Method At first，Bifendate and Oleanolic acid were taken by mice and rats． And then 100 mg /kg and 80 mg /kg ANIT were injected into abdominal cavity of mice and rats to damage their liver，respectively． After 48 hours，their fasting serum levels of ALT，AST，TBIL，DBIL，GGT，ALP were measured． The animals were sacrificed and dissected，and the wet weight of their liver and spleen as well were measured． Finally the viscera index was calculated． Result Oleanolic acid significantly reduced the serum ALT， AST and TB in mice，but Bifendate had no liver protection capacity． Bifendate significantly reduced the serum ALT，AST and ALP in rat，but Oleanolic acid had no liver protection effect． Conclusion Oleanolic acid has liver protection effect on liver injury induced by ANIT in olive oil in mice，and Bifendate has liver protection effect on liver injury induced by ANIT in olive oil in rat．

A Survey on Infection of Parasites and Viruses of the Laboratory Mice and Guinea Pigs in Kunming Area in 2013

2015, 32(01):  25-29. 

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Abstract:Objective To screen the infection of parasites and viruses，including Sendai virus ( SeV)、Mouse hepatitis virus (MHV)、Lymphocytic choriomeningitis virus ( LCMV)，in 90 laboratory mice and 45 laboratory guinea pigs of Kunming area． Method The specimen of 90 mice and 45 guinea pigs were selected from 3 different company randomlies． According to the description of experimental monitor methods of national standard and the protocol of virus assay kit ，parasites and viruses (SeV and MHV of mice ，LCMV and SeV of guinea pigs) of 90 laboratory mice and 45 laboratory guinea pigs were investigated． Result Ectoparasite was detected from 3 animals in 90 mice，detection rate is 3. 3% ． Endoparasite was detected from 20 animals in 90 mice，detection rate is 22. 2% ． Ectoparasite was detected from 4 animals in 45 guinea pigs，detection rate is 8. 9% ． Endoparasite was detected from 9 animals in 45 guinea pigs ，detection rate is 20. 0% ，SeV of mouse and MHV were not detected， LCMV of guinea pigs and SeV of guinea pigs were not detected． Conclusion The situation of virus infection control is quite good，but both ectoparasite and endoparasite infection is relatively common in Kunming area． The works of quality control of laboratory animals should be improved in Kunming area． The quality monitoring of laboratory animals is very important in the biomedical research．

Effects of Different Types of Endurance Training on Oxidative Stress of Myocardial Tissue in Rats

2015, 32(01):  30-33. 

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Abstract:Objective To analyze the effects of different types of endurance training on oxidative stress of myocardial tissue in Sprague-Dawley rats，and thereby to provide rational movement scheme to improve the myocardial antioxidant capacity． Method A total of 48 male SD rats (6 weeks) were enrolled in the study． The study consisted of control group (n = 12)，low intensity exercise group (n = 12)，moderate intensity exercise group (n = 12) and highly intensity exercise group ( n = 12)． The endurance exercise training groups exercised for 6 weeks and the control group maintained their daily eating habits． superoxide dismutase (SOD) activity，glutathione peroxidase (GSH － Px) activity，malondialdehyde (MDA) content were measured after the 6 weeks experiment． Result After 6 weeks endurance exercise training intervention，compared with control group，the SOD activity were higher in moderate intensity exercise group (P ＜ 0． 01)，and significantly higher in highly intensity exercise group (P ＜ 0． 01);the GSH － Px activity were higher in low and moderate intensity exercise group (P ＜ 0． 05 or P ＜ 0． 01)，and significantly higher in highly intensity exercise group (P ＜ 0． 01)． Moreover，the levels of MDA significantly enhanced in highly intensity exercise group (P ＜ 0． 01)． Conclusion The low and moderate intensity exercise group were effective in improve the myocardial antioxidant capacity of rats，particularly in moderate intensity exercise group．

The Installation and Common Trouble Shooting of Pulsation Vacuum Sterilizer in Barrier Environment

2015, 32(01):  34-36. 

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Abstract:This paper describes the principle and structure of pulsation vacuum sterilizer and the installation， common trouble shooting and maintenance of pulsation vacuum sterilizer in use this sterilizer． This will provide some useful information for related laboratory researcher．

Establishment of Risk Assessment System of Pathogenic Microorganism in Laboratory Animal Activity

2015, 32(01):  37-40. 

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Abstract:There exist many potential risk factors concerning the infection of a variety of pathogenic microorganisms in laboratory animals． Therefore，a risk assessment in laboratory animals was carried out from the bio-safety point of view． This paper provides an overview for the risk assessment elements，the risk assessment processes and the risk analysis system． A safety monitoring and early warning system based on the risk assessment was established to ensure the safety of animal laboratory personnel and the environment．

The Breeding Management Method of Genetically Engineered Mice

2015, 32(01):  41-44. 

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Abstract:This article shows our experiences on breeding management of genetically engineered mice in the laboratory animal facilities．

On the Integration and the Development of the Quality System Under the Conditions of Multi-quality Documents

2015, 32(01):  45-47. 

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Abstract:Quality document is an important software content of laboratory construction． Highly integrated and standardized quality documents is a key laboratory to develop a number of quality systems to simultaneously run smoothly． This article describes the CNAS，CMA，CMAF，GLP quality system and quality documentation requirements，as well as the author of the laboratory where the actual file on the quality of integration and the development，practice and experience to achieve a number of specific quality system simultaneously running smoothly．

Advances in Animal Models of Experimental of Rotavirus Infection

2015, 32(01):  48-50. 

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Abstract:Objective To understand the Research progress of Rotavirus in experimental animal models，provide the basis for the further improvement of Rotavirus in experimental animal model． Method At home and abroad in recent years about the Rotavirus infection animal model research literature，the different types of animal models were summarized． Result The class of experimental animal models were analyzed by the research progress，with a view to provide a reference scientific and rational choice Rotavirus scientific research animal models． Conclusion Experimental animal disease animal model of Rotavirus infection of disease occurrence，development mechanism and an essential part of drug development has extremely important role．

Corynebacterium Bovis Infection and Strategies to Prevent and Treat Corynebacterium-Associated Hyperkeratosis

2015, 32(01):  51-54. 

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Abstract:The article discuss about pathogen，epizootiologic features，clinical course，signs，pathological features， possible transmission mechanisms and diagnosis of Corynebacterium bovis infection． And combine research advances of Corynebacterium bovis infection in the field，explore the practical methods to prevent and treat Corynebacterium － associated hyperkeratosis．

The New Research Progress on Establishing Methods of Acute Pancreatitis Animal Model

2015, 32(01):  55-58. 

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Abstract:Acute pancreatitis (AP) is a pathogenesis multiplex disease，clinical process dangerous and lacking of therapeutic drugs． For fundamental research，animal models of AP is a significant substitution model． Although there are a variety of classical establishing methods of AP，preparing of AP model according to different pathogenic factors has become a research hotspot． Therefore，this study reviewed the progress of AP animals model establishment．

Treating Cancer by Regulating the Immune System

2015, 32(01):  59-60. 

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Abstract:Currently，except surgical treatment，we have not found a reliable method of treatment． CTLA － 4 and PD － 1 are two kinds of protein which are produced by T cells． They can puts the brakes on T cells，preventing them from launching full-out immune attacks． By using the monoclonal antibodies of these two protein，the blockerthe CTLA － 4 molecule can be blocked and set the immune system free to destroy cancer． In addition，a personalized treatment that involves genetically modifying a patient’s T cells can make them target tumor cells． It can be used in cancer treatment and is very potential． In this article will make a brief discuss of the above two methods and provide a reference of research in this yield．