Abstract: Abstract:Objective To screen the infection of parasites and viruses，including Sendai virus ( SeV)、Mouse hepatitis virus (MHV)、Lymphocytic choriomeningitis virus ( LCMV)，in 90 laboratory mice and 45 laboratory guinea pigs of Kunming area． Method The specimen of 90 mice and 45 guinea pigs were selected from 3 different company randomlies． According to the description of experimental monitor methods of national standard and the protocol of virus assay kit ，parasites and viruses (SeV and MHV of mice ，LCMV and SeV of guinea pigs) of 90 laboratory mice and 45 laboratory guinea pigs were investigated． Result Ectoparasite was detected from 3 animals in 90 mice，detection rate is 3. 3% ． Endoparasite was detected from 20 animals in 90 mice，detection rate is 22. 2% ． Ectoparasite was detected from 4 animals in 45 guinea pigs，detection rate is 8. 9% ． Endoparasite was detected from 9 animals in 45 guinea pigs ，detection rate is 20. 0% ，SeV of mouse and MHV were not detected， LCMV of guinea pigs and SeV of guinea pigs were not detected． Conclusion The situation of virus infection control is quite good，but both ectoparasite and endoparasite infection is relatively common in Kunming area． The works of quality control of laboratory animals should be improved in Kunming area． The quality monitoring of laboratory animals is very important in the biomedical research．

Key words: experimental animals, mice, guinea pigs, parasites, virus

摘要： 摘要: 目的 分别调查昆明市三家单位提供的共 90 只实验用 ICR 小鼠和 45 只实验用豚鼠的体内、外寄生虫感染 情况，以及小鼠及豚鼠仙台病毒(SeV)、小鼠肝炎病毒(MHV)、豚鼠淋巴细胞脉络丛脑膜炎(LCMV) 的感染情况， 为实验动物质量管理提供一定的参考依据。方法 根据现行国家标准所描述的方法及病毒检测试剂盒说明书的 说明，对昆明市三家单位随机提供的共 90 只 ICR 小鼠和 45 只豚鼠样本，分别进行体内、外寄生虫、小鼠 MHV 及小 鼠 SeV、豚鼠 LCMV 及豚鼠 SeV 的检测。结果 昆明地区实验用小鼠及实验用豚鼠均检出体内、外寄生虫;3 只小 鼠检测出体外寄生虫，检出率为 3. 3% ;20 只小鼠检测出体内寄生虫卵，检出率为 22. 2% ; 4 只豚鼠检测出体外寄 生虫，检出率为 8. 9% ;9 只豚鼠检测出体内寄生虫卵，检出率为 20. 0% ;小鼠 MHV 及 SeV 血清学检测无阳性; 豚 鼠 LCMV、SeV 血清学检测无阳性。结论 实验动物质量监测是实验动物质量控制的有效手段，对医学及生命科学 的研究至关重要，昆明地区实验动物的寄生虫质量控制有待加强。

关键词: 实验动物, 小鼠, 豚鼠, 寄生虫, 病毒