Abstract: Abstract:Objective To observe the protective effects of Bifendate and Oleanolic acid on acute hepatic injury induced by ANIT in olive oil in mice． Method At first，Bifendate and Oleanolic acid were taken by mice and rats． And then 100 mg /kg and 80 mg /kg ANIT were injected into abdominal cavity of mice and rats to damage their liver，respectively． After 48 hours，their fasting serum levels of ALT，AST，TBIL，DBIL，GGT，ALP were measured． The animals were sacrificed and dissected，and the wet weight of their liver and spleen as well were measured． Finally the viscera index was calculated． Result Oleanolic acid significantly reduced the serum ALT， AST and TB in mice，but Bifendate had no liver protection capacity． Bifendate significantly reduced the serum ALT，AST and ALP in rat，but Oleanolic acid had no liver protection effect． Conclusion Oleanolic acid has liver protection effect on liver injury induced by ANIT in olive oil in mice，and Bifendate has liver protection effect on liver injury induced by ANIT in olive oil in rat．

Key words: Bifendate, Oleanolic acid, jaundice type, hepatoprotective effect

摘要： 摘要: 目的 研究蒺藜总螺甾皂苷对人卵巢透明细胞癌荷瘤裸鼠肿瘤的抑制作用。方法 采用人卵巢透明细胞癌 接种裸鼠待长出实体瘤后，用第二代实体瘤按每鼠约 1 mm3 瘤组织块移植裸鼠皮下建立人卵巢透明细胞癌裸鼠模 型;用蒺藜总螺甾皂苷(高剂量 0. 42 mg /mL、中剂量 0. 21 mg /mL)分别给实验组裸鼠灌胃 0. 3 mL/10 g，对照组只给 饮用水，每日 1 次，连续 30 d。每周测量体质量和肿瘤大小 2 次，给药 30 d 后处死小鼠，解剖称肿瘤重量，计算抑瘤 率，评价蒺藜总螺甾皂苷(高剂量、中剂量)对裸鼠荷瘤的抑制作用;结果 蒺藜总螺甾皂苷高剂量和蒺藜总螺甾皂 苷中剂量对人卵巢透明细胞癌荷瘤裸鼠有抑制肿瘤作用，抑瘤率分别 87. 61% 和 95. 26% ，其中中剂量抑制肿瘤与 对照组比较有显著性差异(P ＜ 0. 01)，高剂量组有统计学意义(P ＜ 0. 05);结论 蒺藜总螺甾皂苷(高剂量、中剂 量)均能抑制人卵巢透明细胞癌荷瘤裸鼠肿瘤作用。

关键词: 蒺藜总螺甾皂苷, 人卵巢透明细胞癌, 抑制