Laboratory Animal Science

Genetic Structure Analysis of Three Outbred Beagle Dog Populations Using Microsatellite Markers

JIANG Hui, LI Yinyin , LI Changlong , HUO Xueyun, GUO Meng , CHEN Zhenwen , LI Shengli , LYU Jianyi , DU Xiaoyan

2024, 41(2): 6-14. DOI: 10.3969/j.issn.1006-6179.2024.02.002

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To analyze the genetic structure of outbred populations of small Beagle dogs from variant locations using the selected microsatellite loci, and evaluate if these markers are suitable for genetic detection of the outbred Beagle dog. Method A total of 20 microsatellite markers that has been verified were used to amplifying and STR scanning the sample from 3 companies ( Xi’ an, Beijing and Jiangsu) outbred populations of small beagle dogs by optimized PCR conditions. After genotyping, the genetic indexes were calculated and genetic population structure were analyzed. Result The number of average effective allele of Xi’ an, Beijing and Jiangsu outbred populations of small beagle dogs exhibited with 5. 45, 6. 80, 6. 10, respectively; average heterozygosity was 0. 585 2, 0. 653 8, 0. 663 1, respectively, Shannon’ s information index was 1. 177 4, 1. 384 4, 1. 349 8 and PIC was 0. 539 7, 0. 614 7, 0. 618 1, respectively. It showed that 3 examined population displayed high and abundant genetic population polymorphism and high genetic diversity. The 20 microsatellite loci we used could reflect the general genetic polymorphism character of each population, as well as its unique feature. Conclusion Three outbred populations of small beagle dogs harbor comparably high polymorphism and match the demand of outbred population animal. The set of 20 microsatellite loci we optimized could be adapted for genetically monitoring outbred Beagle dogs.

Regulation of Metabolic Homeostasis in Healthy Dogs by Self-developed Pancreatic Prescription Diet

LIU Yuanyuan, CHENG Xu , CHEN Yun, WAN Juan , HU Manli , ZHANG Xin

2024, 41(2): 15-21. DOI: 10.3969/j.issn.1006-6179.2024.02.003

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Objective This study aimed to prepare a self-developed canine pancreatic prescription diet supplemented with blueberry extract rich in resveratrol and systematically evaluate the regulation and improvement effects on healthy pastoral canine physical status and metabolic homeostasis. Method Healthy dogs fed with self-developed prescription diet or control diet for 8 weeks, respectively. Physical examination, whole blood cell count and serum biochemistry test were carried out in dogs at 0 and 8 weeks of the experiment, and plasma was collected for untargeted metabolomics analysis. Result Compared with the control group, other physiological and biochemical indicators showed no abnormalities in addition to the reduction of total cholesterol in dogs fed with self-developed prescription diet for 8 weeks. Plasma metabolomics showed that self-developed prescription diet feeding promoted glutathione and arginine metabolism in healthy dogs. Conclusion The above metabolic process has been reported to be clearly related to the occurrence of pancreatitis, suggesting the self-developed prescription diet has the potential function of protecting pancreas and regulating metabolic balance.

Study on 2013-2021 Proficiency Testing of Laboratory Animals Quality Monitoring

WANG Hong, WEI Jie, XING Jin, WANG Ji, FENG Yufang, LI Xiaobo, FU Rui, XIAO Jing

2024, 41(2): 22-27. DOI: 10.3969/j.issn.1006-6179.2024.02.004

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Objective To analyze the capacity of the laboratory animal quality monitoring in China, to analyze the organization of proficiency testing of laboratory animal quality monitoring, and to discuss the important factors affecting the satisfaction of proficiency testing. Method To analyze the changes in laboratory numbers, item number, satisfaction rate and participation frequency by using the software Excel2010 和 Powerpoint2010. Result From 2013 to 2021, 27 programs were organized, 22 testing parameters included and 92 biological samples distributed. 70 laboratories participated in 552 cases, and the satisfaction rate reached 90. 22%. Conclusion Continuous participation in the proficiency testing improved the monitoring capacity of the laboratory.

Perioperative Analgesic Effect of Meloxicam in BALB / c Mice Bearing with Tumorectomy

LIU Shijia, GAO Shuolei, QIN Yao, ZHANG Hong

2024, 41(2): 26-34. DOI: 10.3969/j.issn.1006-6179.2024.02.005

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Objective To reduce perioperative pain in mice during tumor resection, ensure animal welfare and the accuracy of research data, the perioperative analgesic effect of meloxicam on tumorbearing mice were explored in this study. Method All BALB / c mice were anesthetized with Zoletil and Dexmedetomidine, and then divided into the control group ( group A) and meloxicam injection group ( group B) . The mouse grimace scale and pain behavior frequency were evaluated to assess the pain level of mice, and the body weight and the histopathology of the organs were observed to test the safety of meloxicam. Result The result showed that compared with group A, the anesthesia induction time and the recovery time in group B were shorter, while the recovery time in group B was significantly longer (P<0. 01) ; the mouse grimace scale and pain behavior frequency in group B were significantly lower than group A within the first 3 days; the main organs and intestinal tissues of all mice had no histopathological lesions, the weight of mice showed no significant difference, and all the mice in group B were survived. Conclusion The result suggested that meloxicam combined Zoletil and Dexmedetomidine anesthesia regimen could shorten the induction of anesthesia and slowly lengthen the recovery time of mice bearing with tumors, significantly reduce their perioperative pain, and be safe.

Establishing a Real-time qPCR Method for Detecting the mRNA Level of ABCG2 in Macaque

LIN Xiaorui, ZHANG Mingrun, WANG Chenyun, ZHOU Wei, YE Yousong, LONG Weihu, LI Zheli, TANG Donghong

2024, 41(2): 35-40. DOI: 10.3969/j.issn.1006-6179.2024.02.006

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:Objective To establish a real-time quantitative PCR method for the detection of mRNA transcription levels of adenosine triphosphate-binding cassette transporter G2 ( ABCG2 ) in macaque (Macaca Mulatta ) . Method We used ABCG2 nucleotide sequence number NM _ 001032919. 1 and internal reference GAPDH nucleotide sequence number NM _ 001195426. 1 of macaque in GenBank database on NCBI. PCR primers were designed using Primer premier 5. 0 software. We extracted total RNA from fresh kidney tissue of rhesus monkey and synthesized cDNA by reverse transcription. Then, PCR primers were used for real-time quantitative PCR amplification, and the relative expression level of ABCG2 mRNA was quantitatively analyzed according to the changes of fluorescence in the reaction system. Result The PCR sequences showed that the homology of the amplified ABCG2 and GAPDH sequences was 90. 91% and 91. 14%, respectively, with that of the NCBI Macaca mulatta sequences. The amplification efficiency of ABCG2 and GAPDH reached 80% - 120%. The meltdown curve of the standard real-time quantitative PCR curve was unimodal, and R2 was close to 1. Conclusion This study established a real-time fluorescence quantitative detection method for ABCG2 mRNA in macaque, which laid a foundation for the study of the pathogenesis of hyperuricemia and the development of new drugs.

Effects of Nicorandil on Reendothelialization of Injured Artery from Animal Model of Balloon Injury in Rat Carotid Artery

WANG Ningyuan, LAI Tuya , WANG Xinyan , LI Huiying , WANG Wei

2024, 41(2): 41-6. DOI: 10.3969/j.issn.1006-6179.2024.02.001

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To investigate the effect of nicorandil on reendothelialization of injured artery from rat carotid artery balloon-injured model. Method A total of 18 Sprague-Dawley ( SD ) rats were randomly divided into 3 groups: sham operation group, control group and experimental group, with 6 rats in each group. The common carotid artery was deendothelialised in the control group and the experimental group, while only the common carotid artery was isolated in the sham operation group. After operation, the control group was given 0. 9% sodium chloride solution ( 0. 5 mL / 100 g, once a day ) , the experimental group and sham operation group were given nicorandil ( 2 mg / 100 g, once a day ) by intragastric administration for 14 days, and common carotid artery samples were collected. The lesion area and reendothelialisation of the vascular intima were observed by Ivans’ blue staining, and the intima hyperplasia and lumen stenosis were observed by hematoxylin-eosin ( HE) staining. Result In the sham operation group, the vascular intima was smooth, the endothelium was intact, and there was no hyperplasia in the vascular intima. After 14 days of balloon injury, endothelial hyperplasia was obvious in control group, and the area of neointimal was significantly higher than that in sham operation group, the difference was statistically significant (P<0. 05) . After 14 days of balloon injury, the area of neointimal in the experimental group was significantly lower than that in the control group, and the proportion of reendothelialization area in the injured arteries was significantly higher than that in the control group, with statistical significance ( P < 0. 05 ) . Conclusion The effects by which nicorandil can facilitate reendothelialization and attenuate intimal hyperplasia of animal model of balloon injury in rat carotid artery are marked

Effect of Fluvastatin Sodium on the Antioxidant System and Liver Function of Zebrafish Liver

WANG Xianrui, MOU Quanling, LI Xiaolong, SHI Jiaye, LI Rumeng, DING Cunbao

2024, 41(2): 41-45. DOI: 10.3969/j.issn.1006-6179.2024.02.007

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Objective To study the effects of fluvastatin sodium on the antioxidant system and liver function in the liver of zebrafish. Method Using a semi-static exposure method, zebrafish were selected as indicator organisms and exposed to blank control group and 0. 004, 0. 04 and 0. 4 mg / L of fluvastatin sodium solution, and samples were taken at 48 h after exposure to detect the effects of fluvastatin sodium on ROS content and activities of antioxidant enzymes SOD, CAT, GPx and GST in zebrafish liver. The activities of liver function indexes ALT and AST were also measured. Result Compared with the control group, the medium and high concentrations of fluvastatin sodium caused a significant increase in ROS content compared with the control group after 48 h exposure ( P < 0. 01 ) , and the activities of CAT, SOD, CuZn-SOD, and GPx changed synchronously, but the low concentration of fluvastatin sodium had no significant activation or inhibition effect on all antioxidant enzymes. Medium concentrations of fluvastatin sodium had activating effects on CAT and SOD ( P < 0. 01 ) , and high concentrations of fluvastatin sodium had activating effects on all antioxidant enzymes ( P<0. 05, P<0. 01) , while high concentrations of fluvastatin sodium had significant activating effects on ALT and AST ( P < 0. 01 ) . The molecular docking result showed that fluvastatin sodium was able to dock with zebrafish CAT protein, with the optimal docking site being Met392 and docking energy of - 4. 63 kcal / mol. Conclusion With increasing drug concentrations, fluvastatin sodium was able to affect the antioxidant system and liver function in zebrafish liver.

Effects of Three Nesting Materials and Placement Levels on the Nesting and Breeding Performance of Mice

PAN Ningjing, ZHU Ye, YANG Jie, SHI Yun, YANG Huixin

2024, 41(2): 46-50. DOI: 10.3969/j.issn.1006-6179.2024.02.008

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Objective To investigate the effects of three nesting materials and placement levels on the nesting and breeding performance of mice. Method C57BL / 6JGpt (B6) mice and BALB / cNj-Foxn1 nu / Gpt (BALB / c-nu) mice were randomly divided into 12 groups ( n = 36, trio-bred) . Mice were provided with three kinds of nesting materials ( raffia, paper cotton and paper tissue) and four placements ( 2 g, 4 g, 6 g and 8 g) levels respectively. Nesting scores were assigned on the first week after mating, and total litter size, total weaning pups and weaning weight of pups were counted weekly for 5 months. Result (1) The nesting scores of the raffia and paper tissue groups were significantly better than those of the paper cotton group in both strains ( P < 0. 05) , and there was no significant correlation between nesting scores and the placement level of nesting material ( P > 0. 05) . ( 2) There was no significant correlation between total litter size, total weaning pups and the kinds of nesting material in both strains (P>0. 05) ; the total litter size and total weaning pups in the 6 g paper tissue group of B6 strain was significantly better than that in the 2 g paper tissue group (P<0. 05) ; the total litter size in the 2 g paper tissue group was significantly better than that in the 6 g paper tissue group (P<0. 05) , and total weaning pups in the 2 g nesting material groups were significantly better than that in the 8 g nesting material groups (P<0. 05) ; there was no significant correlation between weaning weight of pups and types and placement levels of nesting material in both two strains. Conclusion There were differences between the two strains for types and placement levels of nesting materials.

Effect of Three Animal Anesthetics on Anesthetic Efficacy and Serum Biochemical Indicators in C57BL / 6J Mice

LIU Jun, DING Dengfeng, GAO Wei, JIA Yunxiao, ZHAO Changqi, NIU Miaomiao

2024, 41(2): 51-58. DOI: 10.3969/j.issn.1006-6179.2024.02.009

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Objective To compare the anesthetic effects of three animal anesthetics: tribromoethanol, pentobarbital Sodium, zoletil 50 combined with serazine hydrochloride and their effects on serum biochemical indicators in mice. To explore the suitable dose range of three anesthetics for anesthesia of B6 mice. Method 100 healthy SPF grade male B6 mice were randomly divided into 10 groups, with 10 mice in each group. Three types of anesthetics were administered intraperitoneally to B6 mice at high, medium, and low dose gradients, respectively. Compared the induction time, maintenance time, recovery time, and effects on serum ALT、AST、GLU and UA indicators of B6 mice under different doses of three anesthetics. Result The anesthesia maintenance time and recovery time of the three anesthetics were proportional to the dose, while the induction time was inversely proportional to the dose. Medium ( 250 mg / kg) low (125 mg / kg) doses of tribromoethanol (1. 25%) , medium (80 mg / kg) low (40 mg / kg) doses of pentobarbital sodium (1. 0%) , and medium (60 mg / kg) low (30 mg / kg) doses of Zoletil 50 (0. 50%) combined with serazine hydrochloride (0. 50%, medium dose 12 mg / kg, low dose 6 mg / kg) had moderate anesthesia related time and little impact on liver and kidney function in B6 mice. Conclusion The anesthetic effects and serum biochemical indicators of the three anesthetics on B6 mice were different. Tribromoethanol had a suitable anesthesia effect in the range of 125 - 250 mg / kg; Pentobarbital sodium had a suitable anesthetic effect in the range of 40-80 mg / kg; Zoletil 50 combined with serazine hydrochloride had a suitable anesthesia effect within the range of 30-60 mg / kg ( Serazine Hydrochloride 6 - 12 mg / kg) . In studies involving ALT, AST, GLU, and UA, appropriate doses of anesthetics need to be selected based on different experimental needs.

Effects of Different Fixing Solution on Hippocampal Neurons of Golden Hamster under Light Microscopee

GAO Ming, ZHANG Nan, LI Huaiyin, JIN Juan, XIE Peng

2024, 41(2): 59-65. DOI: 10.3969/j.issn.1006-6179.2024.02.010

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:Objective To compare the effects of different fixations on neurons in hippocampus of golden hamster under light microscope, and to provide reference for the selection of fixations in hippocampus of golden hamster. Method 21 11-week-old male SPF golden hamsters were randomly divided into 7 groups. The heart was injected with 4% paraformaldehyde, 10% neutral formaldehyde, Bouin􀆳s, Carnoy, Davidson􀆳s, Zenker and Helly solution, and the whole brain was removed. It was fixed in the corresponding fixing solution for 48 h, and made into paraffin sections. After HE staining, the morphological characteristics of hippocampus neurons were observed under the light microscope. The diameter of neuronal cells was measured by the measuring scale of 3DHISTECH digital slice scanning system in Hungary, and the effect of fixation solution on cell contraction was compared. Result 4% paraformaldehyde, 10% neutral formaldehyde, Bouin􀆳s solution showed a good fixing effect, the fixed brain hardness is moderate, HE stained slices with moderate coloring, bright color, neuron cell morphology contrast is clear, 4% paraformaldehyde is more ideal because the slices without cracking after fixation. Hippocampal neurons immobilized with different fixations showed significant differences in cell shrinkage(P<0. 01) , and the cell shrinkage of neurons immobilized with Carnoy and Davidson􀆳s was the most severe. Conclusion 4% paraformaldehyde, 10% neutral formaldehyde and Bouin􀆳s solution are ideal fixation fluids for hippocampus neurons of golden hammers, and 4% paraformaldehyde solution is recommended as the first choice.

Experimental Study on the Inhibitory Effect of Sodium Danshensu on Ototoxicity of Cisplatin

GUO Na , HU Jun , LIU Ziyi, FU Xiaolong , XIA Ming

2024, 41(2): 66-73. DOI: 10.3969/j.issn.1006-6179.2024.02.011

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:Objective To investigate the inhibitory effect of Sodium Danshensu ( SDSS) on ototoxicity of cisplatin. Method HEI-OC1 cell lines were selected to construct cisplatin-induced damage models based on which protective agents were screened in the small-molecule compound library and Determination of IC50 of cisplatin in HEI-OC1 cells at 24 h. The effect of SDSS on hair cell survival was measured in HEIOC1 cell models and cisplatin-induced mouse cochlear implant models. The effect of SDSS on cisplatininduced oxidative stress levels in HEI-OC1 cells was measured by superoxide anion fluorescence probe (DHE) staining and the positive control was NAC. Result SDSS, a potent protective agent, was selected from 210 FDA-approved small molecule compounds, and SDSS pretreatment significantly inhibited cisplatin-induced decrease in HEI-OC1 cell activity, apoptosis and reactive oxygen species (ROS) aggregation at an optimal concentration of 200 μmol / L. In addition, SDSS significantly inhibits cisplatin-induced damage to hair cells in mouse cochlear explants. Conclusion SDSS may play a protective role in cisplatin-induced ototoxicity by inhibiting ROS aggregation and apoptosis in hair cells.

Stress Effect of Monkey Chair Restraint Training in Rhesus Macaques

ZHAO Qi , , LIU Yongtao, ZHOU Chao , LAN Yanli , WANG Xijie , LI Caiyun

2024, 41(2): 74-77. DOI: 10.3969/j.issn.1006-6179.2024.02.012

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:Objective To investigate the effects of five times in half an hour on stress hormone levels in Rhesus Macaques. Method 3 years old Rhesus Macaques, 15 males and 15 females were divided into three groups, 5 males and 5 females in each group were trained in monkey chair restraint once every two days for 30 min each time. Group 1: no restraint training group, group 2: only restraint training group, group 3: give treats and comfort training group. A total of 5 training times, all the animals in the three groups were collected blood for analysis after each training. Result Compared with group 1, CORT was significantly decreased in groups 2 and 3 after multiple adaptive training of monkey chair preservation (P<0. 05 or P<0. 01) , β-endorphin in group 3 was significantly decreased (P<0. 05 or P<0. 01) , and 5-HT was increased in group 3 at the first and fifth adaptive training ( P<0. 01) . Conclusion When a restraint with monkey chair used in Rhesus Macaques last for half an hour, the adaptive training for the monkey chair restraint can significantly reduce stress. The animals tend to get used to or adapt to the operation of the monkey chair restraint. In adaptive training, positive reinforcement training was given to reduce stress and aggression significantly, and 3-4 times of adaptive training in the monkey chair reduced stress to a constant level.

Literature and Experimental Study on the Establishment of Functional Constipation Model in Rats

XIONG Jing, WANG Fumin , TENG Yuke, GUO Yuyi, ZENG Fang , HE Zhaoxuan

2024, 41(2): 78-84. DOI: 10.3969/j.issn.1006-6179.2024.02.013

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:Objective Based on the result of the literature analysis, we explored the establishment of FC model in rats to provide areference for future animal experiments related to constipation. Method The experiment was divided into two parts. Firstly, 28 rats were randomly divided into blank group ( 8 rats) , compound diphenoxylate group (10 rats) and loperamide group (10 rats) . The blank group was given 0. 9% sodium chloride solution. The experimental groups were given 10 mg / kg compound diphenoxylate or loperamide to establish FC rat model. After 14 days, the differences of the first black stool discharge time, the number of 24 h defecation and fecal water content of the three groups were observed. In the second experiment, thirty rats were randomly divided into blank group, low-dose group and high-dose group. The experimental groups were given different doses of compound diphenoxylate by gavage, and the related gastrointestinal motility indexes were observed after 14 days. Result The result of the first experiment showed that there was no significant difference in the indexes among the three groups (P> 0. 05) ; the result of the second experiment showed that, compared with the blank group, the time of the first black stool discharge was prolonged in the high-dose group, and the fecal water content, the number of 24 h defecation, the rate of small intestinal propulsion, and the rate of gastric emptying were decreased ( P < 0. 05 ) ; compared with the small-dose group, the time of the first black stool discharge was prolonged in the high-dose group, and the fecal water content , 24 h defecation number, small intestinal propulsion rate, and gastric emptying rate decreased, among which the differences in 24 h defecation number and gastric emptying rate were statistically significant ( P<0. 05) . Conclusion Oral administration of 10 mg / kg and 15 mg / kg compound diphenoxylate and 10 mg / kg loperamide failed to establish FC model. 20 mg / kg compound diphenoxylate could establish FC model successfully.

Mechanism of miR-494 Regulating Osteoblast Differentiation and Matrix Mineralization

KUANG Jiabing, GUO Song, LEI Jianping, CHENG Yufang

2024, 41(2): 85-89. DOI: 10.3969/j.issn.1006-6179.2024.02.014

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Objective Effects of miR-494 on osteoblast differentiation and matrix mineralization and related mechanisms. Method mouse MC3T3-E1 osteoblasts were randomly divided into control group, miR-494 over-expression group and miR-494 inhibition group. The control group was not treated, and miR-494 over-expression group and miR-494 inhibition group were transfected with miR-494 MICs transfection reagent and miR-494 inhibitors transfection reagent respectively. ALP activity was detected by alkaline phosphatase ( ALP ) kit, and Osteocalcin ( OC ) activity was detected by osteocalcin radioimmunoassay kit. VonKossa staining was used to detect the number of calcified nodules in each group. The mRNA expression levels of miR-494 and phosphatase tensin homolog gene ( PTEN) were detected by qRT- PCR, the protein expression levels of phosphatidylinositol 3-kinase ( PI3K ) / Aktpathway were detected by Western blot, and the targeting relationship between miR-494 and PTEN was verified by double Luciferase Report experiment. Result Dual luciferase experiments confirmed that PTEN was the target gene of miR-494. miR-494 mRNA and p-Akt protein in osteoblasts with miR-494 over-expression were significantly higher than those in the control group, while PTEN mRNA, ALP and OC activity and calcified nodule number were significantly lower than those in the control group; miR-494 inhibited osteoblast miR-494 mRNA and p-Akt protein were significantly lower than those in the control group and miR-494 over-expression group, while PTEN mRNA level, ALP and OC activity and calcified nodule number were significantly higher than those in the control group and miR-494 over-expression group ( P < 0. 05 ) . Conclusion miR-494 can effectively inhibit osteoblast differentiation and matrix mineralization, and its mechanism may be related to the regulation of Akt pathway by miR-494 mediated target gene PTEN.

Research Progress on Anti-inflammatory Mechanisms of Paeoniflorin

LIU Xinhong , GUO Chao , SONG Bing , ZHAO Hongzhang , WANG Qiong , BAI Min , SONG Wenjing , ZHANG Yu , LIAN Yuan , ZHANG Yanying , ZHAO Xingxu

2024, 41(2): 90-95. DOI: 10.3969/j.issn.1006-6179.2024.02.015

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Inflammation is an abnormal immune response to external stimuli, which can lead to multiorgan tissue dysfunction. Paeoniflorin has been used in Chinese medicine to treat inflammation for nearly a thousand years. Paeoniflorin is the main natural active ingredient extracted from the dried root of Paeoniflorin. It has a wide range of anti-inflammatory and immunomodulatory effects, and has great clinical application potential. In this paper, the anti-inflammatory mechanism and research status of paeoniflorin were reviewed, in order to provide theoretical basis for the anti-inflammatory mechanism of Paeoniflorin and provide reference for further development and application of Paeoniflorin.

Research Progress on Spondyloarthritis Animal Models

ZHANG Jingyi , WANG Youzhi, YANG Fengfan , ZHU Wenxiao , LI Jun

2024, 41(2): 96-101. DOI: 10.3969/j.issn.1006-6179.2024.02.016

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Spondyloarthritis ( SpA) is a common autoimmune disease that mostly affects the axial and peripheral joints. It is characterized pathologically by enthesitis, bone breakdown, and new bone formation. The development of an animal model will provide a good platform for in-depth study of the pathogenesis and therapy of SpA, so this article will review the development of SpA animal model research and strive to provide new ideas for the study and establishment of a SpA animal model, which will further enhance the research theory and experimental basis of the SpA animal model, bringing new ideas for the study of the pathogenesis and clinical therapy of SpA.

Advances in Rodent Models of Preeclampsia and its Pathological Changes

XU Jing , WANG Xiu

2024, 41(2): 102-108. DOI: 10.3969/j.issn.1006-6179.2024.02.017

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Preeclampsia is the second leading cause of maternal mortality, pathophysiological research on the etiology of preeclampsia has long been a hot topic. About the in vivo research in the pre-eclampsia, mainly lies in how to build a animal model, appropriated animal model can be helpful for fully understanding the occurrence and development of the disease and its impact on the fetal litter, domestic and foreign researchers usually use animal models to study, modeling methods generally include biological modeling, surgical modeling, physical modeling, etc. , but a single modeling method still can not completely produce the same animal preeclampsia model as humans, but also need to choose a more appropriate modeling method according to the research purpose and expected results.

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