Abstract: Objective This study aimed to prepare a self-developed canine pancreatic prescription diet supplemented with blueberry extract rich in resveratrol and systematically evaluate the regulation and improvement effects on healthy pastoral canine physical status and metabolic homeostasis. Method Healthy dogs fed with self-developed prescription diet or control diet for 8 weeks, respectively. Physical examination, whole blood cell count and serum biochemistry test were carried out in dogs at 0 and 8 weeks of the experiment, and plasma was collected for untargeted metabolomics analysis. Result Compared with the control group, other physiological and biochemical indicators showed no abnormalities in addition to the reduction of total cholesterol in dogs fed with self-developed prescription diet for 8 weeks. Plasma metabolomics showed that self-developed prescription diet feeding promoted glutathione and arginine metabolism in healthy dogs. Conclusion The above metabolic process has been reported to be clearly related to the occurrence of pancreatitis, suggesting the self-developed prescription diet has the potential function of protecting pancreas and regulating metabolic balance.

Key words: pancreatitis, pancreatic prescription diet, resveratrol, plasma metabolomics; metabolic homeostasis 

摘要： 的 本研究旨在制备添加富含白藜芦醇的蓝莓提取物的自研犬胰腺处方粮,并系统性地评价其对健康田 园犬机体状态和代谢稳态的调节和改善作用。 方法 采用自研处方粮和对照饲料分别喂养健康犬 8 周,对实验 0 周和 8 周犬开展身体检查、全血细胞计数、血清生化检测,并收集血浆进行非靶向代谢组学检测。 结果 与对照组 相比,自研处方粮喂养 8 周犬除总胆固醇降低外,其他生理生化指标未表现出异常,且血浆代谢组学分析发现自研 处方粮可促进健康犬的谷胱甘肽、精氨酸代谢等过程。 结论 上述代谢过程被报道与胰腺炎发生明确相关,提示 自研处方粮具有保护胰腺、调节代谢平衡的潜在功能。

关键词: 胰腺炎, 胰腺处方粮, 白藜芦醇, 血浆代谢组学, 代谢稳态