Abstract: :Objective To compare the effects of different fixations on neurons in hippocampus of golden hamster under light microscope, and to provide reference for the selection of fixations in hippocampus of golden hamster. Method 21 11-week-old male SPF golden hamsters were randomly divided into 7 groups. The heart was injected with 4% paraformaldehyde, 10% neutral formaldehyde, Bouin􀆳s, Carnoy, Davidson􀆳s, Zenker and Helly solution, and the whole brain was removed. It was fixed in the corresponding fixing solution for 48 h, and made into paraffin sections. After HE staining, the morphological characteristics of hippocampus neurons were observed under the light microscope. The diameter of neuronal cells was measured by the measuring scale of 3DHISTECH digital slice scanning system in Hungary, and the effect of fixation solution on cell contraction was compared. Result 4% paraformaldehyde, 10% neutral formaldehyde, Bouin􀆳s solution showed a good fixing effect, the fixed brain hardness is moderate, HE stained slices with moderate coloring, bright color, neuron cell morphology contrast is clear, 4% paraformaldehyde is more ideal because the slices without cracking after fixation. Hippocampal neurons immobilized with different fixations showed significant differences in cell shrinkage(P<0. 01) , and the cell shrinkage of neurons immobilized with Carnoy and Davidson􀆳s was the most severe. Conclusion 4% paraformaldehyde, 10% neutral formaldehyde and Bouin􀆳s solution are ideal fixation fluids for hippocampus neurons of golden hammers, and 4% paraformaldehyde solution is recommended as the first choice. 

Key words: fixing fluid, golden hamster, hippocampal neurons, paraffin section

摘要： 目的 比较不同固定液对光镜下金黄地鼠海马体神经元的影响,为金黄地鼠海马体神经元固定液的选择 提供参考。 方法 将 21 只 11 周龄雄性 SPF 级金黄地鼠随机分为 7 组,分别使用 4% 多聚甲醛、10% 中性甲醛、 Bouin’ s、Carnoy、Davidson’ s、Zenker、Helly 溶液进行心脏灌注,剥离脑壳取出完整大脑,在对应的固定液 中 固 定 48 h,并制作成石蜡切片,经 HE 染色后在光镜下观察海马体神经元的形态特征。 通过匈牙利 3DHISTECH 公司 数字切片扫描系统测量尺测量神经元细胞直径,比较固定液对细胞收缩的影响。 结果 4% 多聚甲醛、10% 中性 甲醛、Bouin’ s 溶液展现了良好的固定效果,固定后的大脑硬度适中,HE 染色后的切片着色适中、色泽鲜艳、神经 元各种细胞形态对比清晰,4%多聚甲醛因固定后切片无龟裂而更为理想。 不同固定液固定后的海马体神经元 存在细胞收缩方面的极显著 差 异 ( P < 0. 01) ,使 用 Carnoy、Davidson’ s 固 定 后 的 神 经 元 各 种 细 胞 收 缩 最 严 重。 结论 4%多聚甲醛、10%中性甲醛、Bouin’ s 溶液是金黄地鼠海马体神经元较为理想的固定液,4%多聚甲醛溶液 建议作为首选。

关键词: 固定液, 金黄地鼠, 海马神经元, 石蜡切片