Abstract: :Objective Based on the result of the literature analysis, we explored the establishment of FC model in rats to provide areference for future animal experiments related to constipation. Method The experiment was divided into two parts. Firstly, 28 rats were randomly divided into blank group ( 8 rats) , compound diphenoxylate group (10 rats) and loperamide group (10 rats) . The blank group was given 0. 9% sodium chloride solution. The experimental groups were given 10 mg / kg compound diphenoxylate or loperamide to establish FC rat model. After 14 days, the differences of the first black stool discharge time, the number of 24 h defecation and fecal water content of the three groups were observed. In the second experiment, thirty rats were randomly divided into blank group, low-dose group and high-dose group. The experimental groups were given different doses of compound diphenoxylate by gavage, and the related gastrointestinal motility indexes were observed after 14 days. Result The result of the first experiment showed that there was no significant difference in the indexes among the three groups (P> 0. 05) ; the result of the second experiment showed that, compared with the blank group, the time of the first black stool discharge was prolonged in the high-dose group, and the fecal water content, the number of 24 h defecation, the rate of small intestinal propulsion, and the rate of gastric emptying were decreased ( P < 0. 05 ) ; compared with the small-dose group, the time of the first black stool discharge was prolonged in the high-dose group, and the fecal water content , 24 h defecation number, small intestinal propulsion rate, and gastric emptying rate decreased, among which the differences in 24 h defecation number and gastric emptying rate were statistically significant ( P<0. 05) . Conclusion Oral administration of 10 mg / kg and 15 mg / kg compound diphenoxylate and 10 mg / kg loperamide failed to establish FC model. 20 mg / kg compound diphenoxylate could establish FC model successfully.

Key words: constipation model, compound diphenoxylate, loperamide, literature study, experiment

摘要： :目的 基于文献分析结果进行功能性便秘( FC)大鼠造模方法的探索,以期为今后便秘相关动物实验提供参 考。 方法 实验分为两部分:(1)将 28 只大鼠随机分为空白组( 8 只) 、复方地芬诺酯组( 10 只) 、盐酸洛哌丁胺组 (10 只) ,空白组灌胃 0. 9%氯化钠溶液,实验组分别采用灌胃 10 mg / kg 复方地芬诺酯混悬液、盐酸洛哌丁胺混悬液 两种方法复制 FC 大鼠模型,14 d 后观察 3 组大鼠首粒黑便排出时间、24 h 排便数及粪便含水率等一般指标的差 异。 (2)30 只大鼠随机分为空白组、小剂量组、大剂量组,每组各 10 只,实验组灌胃不同剂量的复方地芬诺酯混悬 液,14 d 后观察相关胃肠动力指标。 结果 实验一结果显示,3 组大鼠的一般指标差异均无统计学意义( P>0. 05) ; 实验二结果显示,与空白组相比,大剂量组首粒黑便排出时间延长,粪便含水率、24 h 排便数、小肠推进率、胃排空 率降低(P<0. 05) ;与小剂量组相比,大剂量组大鼠的首粒黑便排出时间较长,粪便含水率、24 h 排便数、小肠推进 率、胃排空率降低,其中 24 h 排便数、胃排空率差异有统计学意义(P<0. 05) 。 结论 10、15 mg / kg 复方地芬诺酯及 10 mg / kg 盐酸洛哌丁胺制作 FC 模型失败;20 mg / kg 复方地芬诺酯可成功复制 FC 模型。

关键词:  , 便秘模型, 复方地芬诺酯, 盐酸洛哌丁胺, 文献研究, 实验