Abstract: Objective To study the effects of fluvastatin sodium on the antioxidant system and liver function in the liver of zebrafish. Method Using a semi-static exposure method, zebrafish were selected as indicator organisms and exposed to blank control group and 0. 004, 0. 04 and 0. 4 mg / L of fluvastatin sodium solution, and samples were taken at 48 h after exposure to detect the effects of fluvastatin sodium on ROS content and activities of antioxidant enzymes SOD, CAT, GPx and GST in zebrafish liver. The activities of liver function indexes ALT and AST were also measured. Result Compared with the control group, the medium and high concentrations of fluvastatin sodium caused a significant increase in ROS content compared with the control group after 48 h exposure ( P < 0. 01 ) , and the activities of CAT, SOD, CuZn-SOD, and GPx changed synchronously, but the low concentration of fluvastatin sodium had no significant activation or inhibition effect on all antioxidant enzymes. Medium concentrations of fluvastatin sodium had activating effects on CAT and SOD ( P < 0. 01 ) , and high concentrations of fluvastatin sodium had activating effects on all antioxidant enzymes ( P<0. 05, P<0. 01) , while high concentrations of fluvastatin sodium had significant activating effects on ALT and AST ( P < 0. 01 ) . The molecular docking result showed that fluvastatin sodium was able to dock with zebrafish CAT protein, with the optimal docking site being Met392 and docking energy of - 4. 63 kcal / mol. Conclusion With increasing drug concentrations, fluvastatin sodium was able to affect the antioxidant system and liver function in zebrafish liver. 

Key words: fluvastatin sodium, zebrafish, antioxidant system, liver function, molecular docking

摘要： 目的 研究氟伐他汀钠( FLU)对斑马鱼肝内抗氧化体系和肝功能的影响。 方法 采用半静态暴露法,以氟 伐他汀钠为研究对象,选取斑马鱼为指示生物,将斑马鱼分别暴露在空白对照组和 0. 004、0. 04、0. 4 mg / L 的氟伐他 汀钠溶液中,于暴露 48 h 后,取样检测氟伐他汀钠对斑马鱼肝内 ROS 含量和抗氧化酶 SOD、CAT、GPx、GST 的活 性,同时检测肝功能指标 ALT、AST 的活性。 结果 与对照组相比,氟伐他汀钠暴露 48 h 后,中、高浓度的氟伐他汀 钠会造成 ROS 含量显著性升高( P< 0. 01) ,CAT、SOD、CuZn-SOD、GPx 的活性变化具有同步性,但低浓度的氟伐他 汀钠对所有的抗氧化酶均无显著的激活或抑制作用。 中浓度的氟伐他汀钠对 CAT、SOD 具有激活作用( P<0. 01) , 高浓度的氟伐他汀钠对抗氧化酶 SOD、CAT、GPx、GST 都有激活作用(P<0. 05,P<0. 01) ,同时对 ALT 和 AST 也具有 显著性的激活作用( P < 0. 01) 。 分子 对 接 结 果 表 明 氟 伐 他 汀 钠 能 够 与 斑 马 鱼 CAT 蛋 白 对 接,最 佳 对 接 位 点 为 Met392,对接能量为-4. 63 kcal / mol。 结论 随着药物浓度的升高,氟伐他汀钠能够对斑马鱼肝内抗氧化体系和肝 功能造成影响。

关键词:  , 氟伐他汀钠, 斑马鱼, 抗氧化体系, 肝功能, 分子对接