Table of Content

28 June 2007, Volume 24 Issue 03

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Article

Effect of Fructose on Body Weight and Plasma Glucose in Type Ⅱ Diabetes Mellitus ICR Mice Induced by High Fat Diet and Streptozotocin

NIU Yi-dong,WANG Xin-sheng

2007, 24(03):  1-5. 

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Objective To investigate the effect of fructose on body weight and non-fasting plasma glucose of type Ⅱ diabetes mellitus ICR mice induced by high fat diet and streptozotocin.Methods Twenty ICR mice(21 d) were exposed to high fat diet(HFD),high fat diet-3% fructose(HFDF) and standard rodent chow,respectively.After exposure to the diets for 6 weeks,mice were injected intraperitoneally with either STZ 100 mg/kg body weight or vehicle(0.1 mol/L citric acid buffer,pH 4.5) and kept on the same diet for the next 6 weeks.Body weight was recorded weekly,and non-fasting blood glucose was measured before and at 1～4 weeks post STZ.Results The body weights of HFD-fed and HFDF-fed male mice were significantly higher than those of the chow-fed mice(P<0.05),but there was no significant difference in body weight between HFD-fed and HFDF-fed male mice(P>0.05);body weights of HFDF-fed female mice were significantly lower than those of the HFD-fed mice(P<0.05),and there was no obvious difference in body weights between HFD-fed and chow-fed female mice or between HFDF-fed and chow-fed female mice(P>0.05).The blood glucoses of HFD-fed and HFDF-fed mice were statistically higher than those of chow-fed mice(P<0.01) after exposure to the respective diets for 6 weeks,in both sex,but there was no difference between HFD-fed and HFDF-fed mice.Both blood glucoses of HFD-fed and HFDF-fed male mice were greater than 11 mmol/L(standard value for non-fasting blood glucose of diabetes mellitus model in mice),whereas it was not observed in female mice.Conclusion HFDF-feeding improves increasing of non-fasting blood glucose significantly than HFD-diet in male mice,but it does not improve the development of obesity in diabetes mellitus ICR mice induced by HFD-STZ in both sex.HFDF-feeding is not beneficial to developing type Ⅱ diabetes mellitus model in ICR mice because the concentration of plasma glucose increases too fast.

A Study on the Toxicity of Maca Powder in Mice and Rats

TIAN Hui,FAN Be-Lin,SUN Fan-zhong,YANG Wen-xiang,LIU Yao,FU Shao-hua,TIAN Jie,GONG Chen-rui,WANG Yue,YANG Xian-lun,TANG Li-jun

2007, 24(03):  6-11. 

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Objective Evaluate the security of Maca powder as a health food.Methods The acute toxicity test by oral administration,bone marrow micronucleus test,and sperm shape deformity test were carried out in SPF KM mice were performed,and Maca powder was administered to the Wistar rats for 90 days.Results In the male and female SPF KM mice with oral administration of Maca powder at the total dosage of 10 g/kg(corresponding to 300 times daily dosage in human),the obvious toxic symptoms and death were not found during observing for 2 weeks.The bone marrow micronucleus test,and sperm shape deformity test showed the negative results.In the female Wistar rats with oral administration of Maca powder for 90 days at the low dosage(1.51 g/kg,corresponding to 46 times dosage in human),the middle dosage(3.05 g/kg.corresponding to 93 times dosage in human),and the high dosage(6.16 g/kg,corresponding to 187 times dosage in human,and in the male Wistar rats with oral administration of Maca powder for 90 days at the low dosage(1.42 g/kg,corresponding to 43 times dosage in human),the middle dosage(2.87 g/kg.corresponding to 87 times dosage in human),and the high dosage(5.78 g/kg,corresponding to 175 times dosage in human),no obvious toxic effects were not found by the observations of clinical symptoms,hematological test,biochemical test,changes in weight of organs,and pathological examination.Conclusion The Maca powder can be securely taken as a special food.

The Weight of Main Organs and Organ Coefficient in 10 kinds of Common SPF Rats and Mice

LU Sheng-ming~1,HU Jian-wu~1,CHE Lu-ping~1,WANG Xiao-lu~1,SUN Yan-song~2,SHI Yan-sheng~2,HE Zheng-ming~3,WANG Hong~3,LIU Shuang-huan~3,RONG Rui-zhang~4

2007, 24(03):  12-16. 

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Objective To measure the indexes of normal organ weight and organ coefficient of 10 kinds of usual SPF grade rats and mice.Methods Dissects 10 kinds of strains laboratory animal,determined the normal weight and organ coefficient of the relative weight,measured intestinal tract length with the ruler,which has altogether carried on the determination to 9 kinds of organs coefficient.Results There are showed very significant difference in age-related,sex-related,and strain-related,so on difference existed in the same strains because of of different sexes.

Effects of Goutengsan on Cerebral Blood Flow in Anaesthesia Dogs

HUANG Hou-cai,PENG Yun-ru,DING Yong-fang,WANG Zhi-gang

2007, 24(03):  17-19. 

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Objective Observe the Goutengsan' effects on cerebral blood flow in anaesthesia dogs.Methods Twenty house dogs were randomly divided into 5 groups.Femoral arterial cannula was performed in animals after anaesthesia and then connected to RM-6000 multichannel physiologic recorder.The common carotid artery and vertebral artety were connected to MFV-3200 electromagnetic flowmeter.Goutengsan at high,middle and low dosages were injected into duodenum whereas the positive control group was injected with Nimodipine(2 mg/kg).The changes of cerebral blood flow,systolic blood pressure,diastolic blood pressure,mean arterial blood pressure and heart rate of the dogs before or after giving drug were monitored.Cerebral vascular resistance were calculated.Results In all experimental groups including positive control group the brain blood flow was obviously higher,cerebral vascular resistance was significantly lower than that of control group at 150 min after treatment,whereas the effect of Goutengsan at 180 min was most obvious and continued to 210 min.Conclusion Goutengsan could significantly increase the blood flow of brain,reduce the cerebral vascular resistance,thus improved the brain nutritious condition and prevented the occurrence of senile dementia.

The Replication Characteristics of NDV-Lasota Strain in the CEF Cell

JIN Ji-chang,ZHAO Li-hong~*,QIAO Jian,HU Yong-feng,TIAN Yong,ZHAO Hui-ying,HOU Hai-yan

2007, 24(03):  20-23. 

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The replication characteristics of NDV-Lasota strain in CEF cell were evaluated by cytopathic effects(CPE),hemadsorption test,hemagglutination test,and plaque assay.The results showed that when LH maintenance media supplemented with 25 μg/mL trypsin,the virus replication was effective in CEF and developed dynamicly.The infected cell obtained the hemadsorption ability 24 hour post inoculation(h.p.i),the supernatant of culture medium was positive with HA test 30 h.p.i,obvious CPE appeared 48 h.p.i,and small clear plaque appeared on the monolayer of cell 72 h.p.i.

Pilot Study on Establishing Epidermal Stem Cell Line of Wuzhi Shan Pigs—the Inbred Mini Porcine for Lab Use

CHANG Jie,MOU Yu-lian,LIU Lan,FENG Shu-tang~

2007, 24(03):  24-26. 

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Objective To subculture the epidermal stem cells of Wuzhi Shan Pigs(WZSP) to more than 15 passages so as to establish the epidermal stem cell line of WZSP.Methods The skin pieces from the ear tissue of WZSP were digested with dispase in order to obtain the epidermis,and then the epidermis was dissociated into single cells with 0.25% tripsin and 0.02% EDTA.Then the cells were inoculated onto human collagen Ⅳ coated flasks and cultured at 37℃ in a humidified atmosphere containing 5% CO_2.The cells which didn't adhere were rinsed off 10～15 minutes after inoculation.The adherent cells were cultured in the conditional medium [including DMEM/F10(1∶1),10%conditioned medium,5 μg/mL insulin,20 ng/mL EGF,20ng/mL IGF-Ⅰand 0.5 μg/mL hydrocortisone],and subcultured with 0.25% tripsin.ESCs colonies were stained by AKP.Results With phase contrast microscope,the rapidly adherent cells were observed to form colonies 24 hours after inoculation.The ESCs have been subcultured to more than 15 passages,and the interval was about 7 days.The ESCs colonies which were stained by AKP showed red color.Conclusions It is possible to establish the ESCs line in the condition of this experiment.AKP identification demonstrates that the ESCs have the characteristics of stem cells.

Anaesthetic Effectiveness between Specially-Made Minnaining and the Ketamine Hydrochloride in Guangxi Bama Minipigs

QIAN Jiang-nan~1,ZHOU Ping~1,XIE Man-hong~1,WANG Dong-gang~1,LIU Jia-Chun~2

2007, 24(03):  27-29. 

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Objective To investigate the effect of narcosis between specially-made minnaining and the ketamine hydrochloride in Guangxi Bama minipigs.Methods Fifteen Bama minipigs were injected into specially-made Minnaining and the ketamine hydrochloride,the forward and backward process of experimentation and the evaluatation was complete identity.Results Anaesthesia with ketamine hydrochloride,only two cases accomplished the operations.Anaesthesia with specially-made Minnaining,most of the pigs were caused narcosis quickly.Conclusion Compare to ketamine hydrochloride,the effect of narcosis about specially-made Minnaining has advantage more.

An Observation on Effectiveness of Guliben Powder(Calcium Ions Powder) Injection in Miniature Pigs Grown in Guangxi

HUANG Wei-jian~1,YIN Ye-shi~1,CHAN Jian-chao~1,WANG Ai-de~

2007, 24(03):  30-32. 

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Objective This research focus on possibility for intravenous injection,intramuscular injection and intradermal injection of ionized calcium of Guliben in Guangxi Bama minipigs.Methods Selecting Guangxi Bama minipigs as test animals,one minipigs first intravenous injection of noradrenalin which can damage cardiac muscle 12 times(2 mg/kg\5wt),then intravenous injection of ionized calcium of Guliben later;two minipigs inject different concentration diluents(normal saline,glucose normal saline,distilled water)and different concentration the ionized calcium of Guliben.The injection methods contain intravenous injection,intramuscular injection and intradermal injection;control minipig inject normal saline and observe the response.Results In the process of intravenous injection of onized calcium of Guliben.Most of the test animals show reduce of the rate of heartbeat and respiration,pigs don't have any adverse reactions,quiet;ionized calcium of Guliben diluted by distilled water is preferable; the proper concentration of the ionized calcium of Guliben is 2.3 mg/mL.In the process of intravenous injection of noradrenalin,the test animal show increase of the rate of heartbeat and respiration,feel affliction and have acuate response,but intravenous injection of ionized calcium of Guliben later,the pigs feel comfort and quiet.Intramuscular injection and intradermal injection of ionized calcium of Guliben don't have adverse reactions.Conclusions From this research,we can affirm that the ionized calcium of Guliben can used in intravenous injection,intramuscular injection and intradermal injection.

Effects of The Immunoglobulin IgG,IgA,IgM to Add Mucaosu in Basic Diets of Young Rhesus Monkeys

ZHAO Yuan,HE Zhan-long,CHEN Li-xiong,WANG Jun-bin,LU Shuai-yao

2007, 24(03):  33-35. 

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Objective To measure and analyse the immunoglobulin IgG,IgA,IgM in young rhesus monkeys feeding basic diets supplemented with Mucaosu.Methods Fifteen health and young rhesus monkeys were divided into group A,B,C.Group A was control group,group B and group C were fed with diet containing 0.1% and 0.2% Mucaosu,respectively.Two months later,IgG,IgA,IgM were measured.Results IgG levels in group A,B,C were 644.8 mg/dL±72.23 mg/dL,756.4 mg/dL±55.87 mg/dL,776.4 mg/dL±193.84 mg/dL,respectively;IgA were 12.41 mg/dL±3.31 mg/dL,17.2 mg/dL±1.77 mg/dL,19.75 mg/dL±0.72 mg/dL respectively;IgM were 42.66 mg/dL±4.45 mg/dL,65.58 mg/dL±10.11 mg/dL,71.36 mg/dL±10.62 mg/dL respectively.Conclusion The contents of immunoglobulin IgG,IgA,IgM of group B and group C were significant higher than group A(P<0.05);IgG,IgA,IgM of group C were much higher than group B(P<0.05).The finding suggested that Mucaosu had effects on development the immunoglobulin IgG,IgA,IgM in young rhesus monkeys.

Rapid Detection of Simian Parainfluenza Virus 5 by RT-PCR

WU Fan,HE Zheng-ming~

2007, 24(03):  56-59. 

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To detect the presence of Simian parainfluenza virus 5(SV5) in laboratory animals and related biological products or materials,a pair of primers that amplify the SH gene were designed and synthesized.After the best reaction conditions were tested out,RT-PCR technique was established for the detection of RNA of SV5.By nucleic acid sequence analysis,sequence homology of RT-PCR positive fragment and the reported SV5 WR-21005 strain achieved 97%,which testified that the method was specific for the detection of SV5 nucleic acid sequence.Sensitivity of the method was determined as the minimum RNA concentration of 2.1 ng/μL and the minimum virus titer of 3.98CCID_(50)/0.1 mL.For initial application of the method,samples including organs of simian and hamster's,biological products from simian(OPV) and five commonly used passage cells from simian kidney were tested by the RT-PCR method for the SV5 nucleotide gene.Results came out that no SV5 nucleotide gene was detected in above specimens.The results showed that the SV5 RT-PCR technique we established provided a diagnosis method of SV5,and also a technique for SV5 molecular epidemiologic survey.

A New Examining Method of Intestinal Transmission Function of Mice

QIAN Ning,WU Quan-sheng,WU Shu-guang,GUO Ke-nan,DONG Xiao-jun

2007, 24(03):  60-62. 

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Objective To Delve into application of examining intestinal transmission function of mice by infusing stomach with barium sulfate.Method Barium sulfate and activated carbon were infused into stomachs of the mice in the normal and the constipation groups,and the intestinal moving rate,stool quantity,and first defecation time were observed.Result The intestinal moving rate,stool quantity,and first defecation time in the normal and the constipation mice were objectively demonstrated by infusing stomach with barium sulfate.Conclusion The infusing stomach with barium sulfate can be used as a new examining method of studying intestinal motility in mice.

Comparative Study of The Three Methods of Confirming Estrous Cycle of Mice

LI Jing~

2007, 24(03):  63-64. 

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In order to choose the best method of confirming estrous cycle of adult Kunming female mice.Three methods were used in order to confirm the estrous cycle,including cervix mucus crystalisation,vaginal exfoliative cell smear and vaginal exfoliate cell counting.The results showed that all three methods can confirm the estrous cycle of mice,but specificity was lower in the first method,there was false negativity in the second method and the third method was convenient and accurated.