Abstract: Objective To investigate the effect of fructose on body weight and non-fasting plasma glucose of type Ⅱ diabetes mellitus ICR mice induced by high fat diet and streptozotocin.Methods Twenty ICR mice(21 d) were exposed to high fat diet(HFD),high fat diet-3% fructose(HFDF) and standard rodent chow,respectively.After exposure to the diets for 6 weeks,mice were injected intraperitoneally with either STZ 100 mg/kg body weight or vehicle(0.1 mol/L citric acid buffer,pH 4.5) and kept on the same diet for the next 6 weeks.Body weight was recorded weekly,and non-fasting blood glucose was measured before and at 1～4 weeks post STZ.Results The body weights of HFD-fed and HFDF-fed male mice were significantly higher than those of the chow-fed mice(P<0.05),but there was no significant difference in body weight between HFD-fed and HFDF-fed male mice(P>0.05);body weights of HFDF-fed female mice were significantly lower than those of the HFD-fed mice(P<0.05),and there was no obvious difference in body weights between HFD-fed and chow-fed female mice or between HFDF-fed and chow-fed female mice(P>0.05).The blood glucoses of HFD-fed and HFDF-fed mice were statistically higher than those of chow-fed mice(P<0.01) after exposure to the respective diets for 6 weeks,in both sex,but there was no difference between HFD-fed and HFDF-fed mice.Both blood glucoses of HFD-fed and HFDF-fed male mice were greater than 11 mmol/L(standard value for non-fasting blood glucose of diabetes mellitus model in mice),whereas it was not observed in female mice.Conclusion HFDF-feeding improves increasing of non-fasting blood glucose significantly than HFD-diet in male mice,but it does not improve the development of obesity in diabetes mellitus ICR mice induced by HFD-STZ in both sex.HFDF-feeding is not beneficial to developing type Ⅱ diabetes mellitus model in ICR mice because the concentration of plasma glucose increases too fast.

Key words: Fructose, High fat diet, Streptozotocin, ICR mice, Diabetes mellitus

摘要： 目的观察果糖饮水对高脂饲料-STZ诱导ICR小鼠Ⅱ型糖尿病模型过程中体重及血糖浓度变化的影响。方法选取ICR小鼠(3周龄)雌雄各半,先以高脂饲料及高脂饲料加3%果糖饮水喂养6周,再以链脲佐菌素(STZ)(100 mg/kg体重)腹腔注射,继续喂养6周。每周测定体重,于注射前及注射后4周测定非空腹血糖浓度。结果雄性高脂饲料-果糖饮水组和高脂饲料组体重显著高于对照组(P<0.05),而高脂饲料-果糖饮水组与高脂饲料喂养组间没有显著差异(P>0.05);雌性高脂饲料-果糖饮水组和高脂饲料组体重与对照组均无显著差异(P>0.05),但高脂饲料-果糖饮水组体重显著低于高脂饲料组(P<0.05)。喂养6周后(注射STZ前),高脂饲料-果糖饮水组和高脂饲料组的血糖浓度均显著高于对照组(P<0.05),而实验组间没有显著差异。注射STZ后,雄性高脂饲料-果糖饮水组和高脂饲料组的血糖浓度均显著高于对照组(P<0.01),并且都超过糖尿病小鼠非空腹血糖的成模标准(11 mmol/L),且高脂饲料-果糖饮水组血糖浓度又显著高于高脂饲料喂养组(P<0.05);雌性小鼠高脂饲料-果糖饮水组和高脂饲料组血糖浓度均显著高于对照组(P<0.01),但都没有达到糖尿病小鼠非空腹血糖的成模标准,且高脂饲料-果糖饮水组与高脂饲料组间血糖浓度没有显著差异(P>0.05)。结论在高脂饲料喂养联合腹腔注射STZ制备Ⅱ型糖尿病模型实验中,果糖饮水对高脂饲料致肥胖作用没有显著的改善,而对血糖浓度升高具有明显的促进作用。这种促进作用在雄性小鼠最为明显,而对雌性小鼠没有明显效果。

关键词: 果糖, 高脂饲料, 链脲佐菌素, ICR小鼠, 糖尿病