Table of Content

25 March 2004, Volume 21 Issue 01

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Article

Observation and Analysis of The Reproductive Capacity and The Breeding Regularity of Different Foetal Numbers in BALB/c Nude Mice

LIAN You_wen,MO Mei_ying,CHEN Rong_hua,ZHONG Xiu_xia,LI Shi_fu

2004, 21(01):  1-3. 

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Objective\ To observe and analyze the reproductive capacity and the breeding regularity of different foetal numbers in BALB/c nude mice.Methods\ 160 seed mic e (80 male mice and 80 female mice)were observed and statistically classified acco rding to the data of the breeding cards.Results\ The pregnant rates of BALB/c nude mice was 95%.The average foetal number of each female nude mouse was 3.09±1.23 and the average breeding number was 7.21± 2.74. The number of all homozygotes was 50.23 percent of all breeding number and the average homozygote number of each nest was 3 64±1 57.The wean number of homozygotes was 88.32 pe rc ent of homozygote number and the average wean number of each nest was 3.22±1.83 .The reproductive capacity was the best at the second and the third foetal numbe r,took second place at the first and the fourth foetal number and was the worst at the fifth foetal number.Conclusion\ The breeding of BALB/c nude m ice is fine in the third grade barrier system,but seed mice should be changed af ter the third or the fourth foetal number is bred.

Blood Fat Examination of Experimental Rat

ZOU Yi_hai,CHEN Jia,XIE Ling_ling,SUN Qi,FU Lu_di

2004, 21(01):  4-5. 

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ObjectiveTo assess the total cholesterol (TC) and triglyceride (TG) of the conventional a nd clean SD rats.Method\ CHO_PAP Method was used to determine TC;GPO _PAP Method was used to determine TG.Result\ (1)The result of the fe male conventional SD rats was TC:(1.67±0 24)mmol/L;TG:(0.52±0.12)mmol/L.( 2)The result of the male conventional SD rats was,TC:(1.52±0.18)mmol/L;TG:(0.5 8±0.14)mmol/L.(3)The result of the female clean SD rats was,TC:(1.80±0.32)mmo l/L;TG:(0.43±0.09)mmol/L.(4)The result of the male clean SD rats was,TC:(1.60 ±0.42)mmol/L;TG:(0.75±0.30)mmol/L.Conclusion\ The experiment provi des reference to set up the standard of blood biochemistry of normal clean labor atory animal.

Consecutive Changes of HA, LN in BALF and Vein in Blemycine-induced Pulmonary Fibrosis in Rats

XU Po

2004, 21(01):  6-8. 

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Consecutive changes of HA(hyalnronic acid),LN(Laminin) in BALF and veno us blood were observed in blemycine induced pulmonary fibrosis in rats and correlated to histopathologica l change.Our results showed:(1)The HA level of the vein and BALF on the 7\+\{th\} day in the model group was increased (\%p\%<0 05)or significantly increased (\%p\%<0 01) than those in the normal control group.(2)The LN levels from vein on the 7\+\{th\} d ay and 28\+\{th\} day in model group were higher significantly than those in the normal control g roup (\%p\%<0 01);there was no significal difference of the BALF and LN levels on the 7\+\{th\} day and 28\+\{th\} day .(3) HA and LN acummulation in vein and BALF preceded the collagen deposition in the lung. Our results signified that increa sed HA and LN level occurred in the early stage, and their falling reflected the presence of fibrosis. HA and LN, particularly HA, are the sensitive index t o reflect the early stage of pulmonary fibrosis(lung injury stage)

Effect of Two Different Pregnancy Methods on Productive Capacity of Mice

ZHANG Jin_song,LENG Yang

2004, 21(01):  9-11. 

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ObjectiveTo compare the two productive methods,cocurrent pregnancy mice and general pregn ancy ones,in offspring production.Methods\ SD famal rats (5 monthes o ld)were divided into two groups,one of which was the experiment group (cocurrent pregnancy) and the other was control group(general pregnancy).All rats mated in 1∶1(♀∶♂)and continued to be observed for three pregnancies.Results\ Average litter size was 9.30 in experiment group and it was 8.07 in general group.In this period,the number of cocurrent pregnancy rats with mammary tumour reduced generally.Conclusions\ Cocurrent pregnancy is an efficient method in improving the output rate of rats.

Therapeutic Effects of Shuang-Huang-Qing-Kou Capsule for Mouth Ulcer

WANG Ting, SONG Huai\|yan, ZHANG Wen\|ming

2004, 21(01):  12-14. 

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Objective\ To observe the therapeutic eff ects of Shuang\|Huang\|Qing\|Kou capsule for mouth mucous ulcer.Methods\ (1)SD rat weighted 150～180g were divided into:the high\|,the midlle\|,the low\|dosage(5 40,2 70,1 35g crude/kg),the positive drug contro l and the negative control group.The drugs were administered(i.g)daily for 14 da ys,the mainly drug effect of Shuang\|Huang\|Qing\|Kou capsule was examined.(2)KM mice weighted 18～20g were divided into:the high\|,the midlle\|,the low\|dosage (7 20,3 60,1 80g crude/kg),the positive drug control and the negative contro l group.The drugs were administered(i.g)daily for 7 days,the effect of Shuang\|H uang\|Qing\|Kou capsule was examined by anti\|inflammation test and heated\|plat e test.Results\ (1)Shuang\|Huang\|Qing\|Kou capsule can reduced the diameter of ulcer area,which showed a significant difference( P <0 01, P <0 05)in every dosage group compared with negative control group.(2)Shuang\|Hu ang\|Qing\|Kou capsule can reduced the inflammatory edema inducing by xylene on the ear of mice,and depressed swell rate( P <0 01, P <0 05)significantl y.(3) Shuang\|Huang\|Qing\|Kou capsule can reduced pain response which causing by hea t irritation,and raise pain region rate( P <0 01, P <0 05)significantly .Conclusion\ Shuang\|Huang\|Qing\|Kou capsule can promoted recoverin g the mouth mucous ulcer,also have a better anti\|inflammation effect and reliev e a pain effect.

Effects of Gen-Seng in Different Solvents on Liver Glycogen and Related Parameters

GAO Shan, TONG Ying, WU Shao_ping, XIONG Xiao_yan

2004, 21(01):  15-17. 

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Objective\ To study effects of gen_seng in different solvents on liver glycogen and related parameters. Methods: Mice were given 33 3,333 3 and 1000 0 mg/kg gen_seng product in water and oil,res pect ively,30 d later,liver glycogen,blood glucose,triglyceride (TG),very low density lipoprotein (VLDL),alanine aminotransferase(ALT) were measured.Results\ 33 3 mg/kg of Gen_seng in water increased liver glycogen significantly ( P <0 05),and TG,VLDL significantly decreased ( P <0 05)in 333 3 and 1000 0 mg/kg groups.Gen_seng in oil decreased ALT in 33 3, 333 3 and 1000 0 mg/kg groups( P<0 05,P<0 01 respectively),and had no effects on liver glycogen an d TG. Compared with water,oil improved ALT and blood sugar ( P <0 05),and dec reased TG and VLDL ( P <0 01).Conclusion\ Oil can affect the mech anism of sugar and fat metabolisms,therefore,we should choose solvents carefully .

The Experimental Study Development on Animal Model in Depression of The Liver-Qi and Insufficiency of The Spleen-Qi Syndrome

PENG Yan juan, PENG Cheng

2004, 21(01):  32-34. 

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The experimental study on animal model in the depression of the Live Qi and ins ufficiency of the Spleen Qi syndrome in recent years was reviewed, and ana lyzed the problems in it. The methods of making model were as follows:CCl\-4 inj ection, combining high fat diet with CCl\-4 injection, combining the irritating rats by clipping the tails with purging by medicines bitter in taste and cold in nature,combining the limiting diet and movement with coloclyster by acetic acid to make model of the TCM syndrome or ulcerative colitis with the the TCM syndro me. These methods opened the new pass to study this syndrome further. But there were still several problems in these model studies, which should be improved. Fo r example: the etiological similarity with the TCM syndrome, establishing the di agnosis standard and biological feature according to the animal physiological and pathological characteristic, comparing the test index each other, standardi zing the treatment fomular and herbs.

The Research Survey of Experimental Animal Model about Chronic Atrophic Gastritis

ZHU Fang shi, SI Jian min

2004, 21(01):  35-37. 

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The status of making experimental animal models abou t chronic atrophic gastritis have been surveyed and synthesis in respect of phys ical a nd chemical factors, immunotherapy, surgical method and synthesis. The making of CAG experimental animal model should be quick, high effective, steady, reliab le and economical.

Survey of Microorganisms Infection in Monkeys from 1998 to 2001

FAN Wei, YANG Jing, SUI Li_hua, TIAN HAO, ZHENG Zhen_feng, SUN Yan_song

2004, 21(01):  47-50. 

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Several microorganisms infected monkeys were detected in 1998～2001. The results showed that no \%Salmonella,Pathogenic dermal\% fungi, \%Mycobacterium tubercu losis\% and antibodies to SRV,SIV,STLV_1,HBV were detected.\%Shigella\% and an tibodies to BV,MV,(HAV)were found.

Detection of Staphylococcus aureus DNA in Paraffin-embedded Specimens by Polymerase Chain Reaction

GAO Zheng_qin, XING Hua, SUN Huai_chang, LI Hou_da

2004, 21(01):  51-52. 

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Objective\ To pvovide an additional diff erential diagnostic method for pathological diagnosis of Staphylococcus aureus (S.aureus) infection in formalin_fixed and paraffin_embedded tissue samples b y Pol ymerase Chain Reaction(PCR). Methods\ 55 tissue samples of formalin_ fixed and paraffin_embedded tissue samplex of laboratory mice pathologically dia gnosed as S.aureus inflammation were examined by PCR. Results\ Among them, 55 tissue samples all showed positive results:the specific bands of 678bp were present in the agarose gel. Conclusion\ The results showed the PCR assay developed is a reliable method and very useful for retrospective stud y of S.aureus infection on the molecular level.

Heart Puncture for Collecting Blood:The Analysis of 160 Mice

WU Xiao qing 1, HAO Chen xia 2

2004, 21(01):  53-54. 

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Objective\ To summarize the experience in the heart puncture for collecting blood of 160 mi ce. MethodsMice were anesthetized with ketamine hydrochloride, then the heart punctures were performed by 1ml syringes.ResultsThe amount of blood collected was between 0 8～1 1mL in a heart of each mouse. ConclusionIt is the best method for collecting blood by the heart punctures in mice.