Abstract: Consecutive changes of HA(hyalnronic acid),LN(Laminin) in BALF and veno us blood were observed in blemycine induced pulmonary fibrosis in rats and correlated to histopathologica l change.Our results showed:(1)The HA level of the vein and BALF on the 7\+\{th\} day in the model group was increased (\%p\%<0 05)or significantly increased (\%p\%<0 01) than those in the normal control group.(2)The LN levels from vein on the 7\+\{th\} d ay and 28\+\{th\} day in model group were higher significantly than those in the normal control g roup (\%p\%<0 01);there was no significal difference of the BALF and LN levels on the 7\+\{th\} day and 28\+\{th\} day .(3) HA and LN acummulation in vein and BALF preceded the collagen deposition in the lung. Our results signified that increa sed HA and LN level occurred in the early stage, and their falling reflected the presence of fibrosis. HA and LN, particularly HA, are the sensitive index t o reflect the early stage of pulmonary fibrosis(lung injury stage)

Key words: Blemycine, HA, LN, Consecutive changes

摘要： 观察了博莱霉素 (BLM )致肺纤维化大鼠血和支气管肺泡灌洗液 (BALF)中透明质酸 (HA)和层粘连蛋白 (LN)含量的动态变化及肺组织病理变化 ,并与正常大鼠对照。结果表明 :(1)血清和BALF中的HA含量制模第 7天时高于(P <0 0 5)及明显高于 (P <0 0 1)正常对照组 ,第 2 8天时 ,开始回落 ;(2 )血清中LN含量在第 7天及第 2 8天时均明显高于 (P <0 0 1)对照组 ;而BALF中LN含量无明显变化 (P >0 0 5) ;(3 )对比肺脏病理变化 ,HA和LN增加先于肺内胶原合成。因此认为HA和LN为反映肺间质纤维化较为敏感的早期指标 ,尤其HA在血清和BALF中早期均增高 ,而肺纤维化期逐渐回落 ,可作为肺纤维化早期肺泡炎阶段的标志物之一

关键词: 博莱霉素, 透明质酸, 层粘连蛋白