Abstract: Feed and bedding, as key factors in experimental animal research, have a significant impact on the experimental results, but their standardization has not been paid enough attention for a long time. In this paper, we systematically reviewed the mechanisms of feed texture, processing technology and sterilization methods on nutritional metabolism, intestinal microbiota and physiological indicators of experimental animals, and revealed the reproducibility effected by the type and volume of bedding through thermal regulation, behavioral stress and microbial interference. Based on this, this paper proposes that researchers should consider the influence of these factors when conducting relevant experiments to eliminate controllable variability, and suggests the establishment of a feed and bedding standardization system covering the bioavailability of nutrients and processing parameters, and encourages researchers to disclose relevant parameters in research reports to improve the scientific and reproducibility of related studies.

Key words: laboratory animal, feed, bedding, metabolomics, standardization

摘要： 饲料与垫料作为实验动物研究中的关键因子对实验结果具有显著影响,但其标准化问题长期未受到足够重 视。 本文系统综述了饲料质地、加工工艺及灭菌方式对实验动物营养代谢、肠道菌群和生理指标的影响机制,同时 揭示了垫料类型与垫料体积通过热调节、行为应激和微生物干扰等途径影响实验结果的可重复性。 基于此,本文 提出科研工作者在进行相关实验时应考虑这些因素造成的影响,以消除可控制的变异性,并建议建立涵盖营养成 分生物利用度及加工工艺参数的饲料和垫料标准化体系,鼓励科研人员在研究报告中披露相关参数,以提升相关 研究的科学性和可重复性。

关键词: 实验动物, 饲料, 垫料, 代谢组学, 标准化