Loading...
Authors Center
Submission or Manuscript
Information for Authors
Template
Copyright Agreement
Review Center
Peer Review
Office Work
Links
Table of Content
28 June 2010, Volume 27 Issue 03
Previous Issue
Next Issue
Article
Isolation,Culture and Related Biological Characteristics of Bovine Fetal Fibroblasts
YIN Ming1,ZHANG Yi-zhe1,DUO Shu-guang2
2010, 27(03): 1-6.
Asbtract
(
)
Related Articles
|
Metrics
The bovine fetal otic fibroblast cells were isolated using tissue bulk attachment. The gender of fetus was identified using PCR. The fibroblast cells from female fetus were chosen for further morphologic observation,growth curve and chromosome analyzing. The results indicated that the cells had normal morphology,multiplication characteristics and chromosome number. The establishment of culture method and related biological characterization analysis of bovine fetal fibroblast provided not only a ready supply of somatic cells for the intraspecies and interspecies cloning of macaque but also fundamental conditions for somatic transgene and other related fields.
Study on Isolation and Culture of Mouse Embryonic Fibroblast
CHEN Ming-yu
2010, 27(03): 7-9.
Asbtract
(
)
Related Articles
|
Metrics
Objective To explore the isolation and culture of mouse embryonic fibroblasts ( MEF). Methods The MEF were isolated from the embryos of BALB /c mouse using in vitro culture system,the growth morphology of the MEF were observed,and the passage cell culture medium,embryonic gestational age,the trypsin concentration were screened. Results The MEF in vitro was a kind of adherent cell,the third,fourth,fifth cell proliferation with high purity and the most exuberant,adding serum M199 and DMEM can satisfy the growth of primary cells, the speed of proliferation had no significant differences in two cell culture medium; the optimal gestational age for isolation of mouse embryonic fibroblasts was from 11. 5 to 16. 5 day. The optimal time for digesting MEF was 8 ～ 11 min with 0. 1% of trypsin. Conclusion By selecting the factors which influence of isolation and culture laid the foundation for further study of the MEF.
The Histological Observation in the Testis of Mice at Different Development Stage
LUO Lan,ZHANG Yan,YANG Fang,YANG Yu-ling
2010, 27(03): 10-13.
Asbtract
(
)
Related Articles
|
Metrics
To observe the development of testis in mice,the testis of total 17 groups of Kunming normal mice at their different developmental stages among 1 ～ 57 days after birth was examed by using the paraffin tissue section and HE staining. The result showed that only sertoli cells and primitive germ cells exhibited in seminiferous tubules of the early postnatal mice testis; B-type spermatogonia were observed at the 8th day after birth; Primary spermatocytes were observed at the 15th day; Secondary spermatocyte and little round spermatids appeared at the 23th day and then the quantity of round spermatids were gradually increased. At the 30th day,long-shaped sperm cells exhibited. At the 36th day,a large sum of sperms were observed in seminiferous tubules and lasted to whole subsequent stages. It means that via a process of continuously development and differentiation the primitive germ cells matured in about 36 days after birth. This study revealed more details on the development of mice testis and sperms.
Establishing the Strangulated Intestinal Obstruction Model in Rats
BAI Yang,FU Wei,YUAN Guo-hong,TAO Ming,ZHANG Tong-lin
2010, 27(03): 14-18.
Asbtract
(
)
Related Articles
|
Metrics
Objective To establish a rat model of strangulated intestinal obstruction,which can present good time-dependent relation and unification between the intestinal incarceration time and the degree of intestinal necrosis. Methods By choosing adult healthy male Spraque-Dawley rats,an incarceration hernia model to imitate the strangulated intestinal obstruction was made. In the incarceration hernia model,a midline fascial defect was made,the length of the defect was set by the 80 percent of the diameter of incarceration bowel,and the length of incarceration bowel was 6 cm. All rats were divided into six groups by different intestinal incarceration time,from 1 hour to 6 hours step by 1 hour. With the sham operation group as the contral group,the grade of intestinal injury between the groups was compared by necrosis degrees under microscopy,and the statistic method was ritid analysis. Results Comparing with the control group,the different degree of intestinal necrosis was found,the longer intestinal incarceration time and the higher degree of intestinal necrosis. But with the same intestinal incarceration time,the degree of intestinal necrosis was similar. The mean ritid value of control group was 0. 038; the mean ritid value of each experimental group from 1 hour to 6 hours was 0. 248,0. 329,0. 464,0. 551,0. 765 and 0. 874,respectively. Using LSD test of analysis of variance,microscopic intestinal injury in each eaperimental group was significantly different from the control group(P < 0. 035); the significantly difference of intestinal injury was found between the experimental groups,which difference of intestinal incarceration time was 2 hours or more(P > 0. 09); no significantly difference of intestinal injury was found between the experiment groups,which difference of intestinal incarceration time was 1 hours,except for the 4 hours group and 5 hours group(P = 0. 01). Conclusion A rat model of strangulated intestinal obstruction has been established,which can present good time-dependent relation and unification between the intestinal incarceration time and the degree of intestinal injury.
Experimental Study of Inhibitory Effect on Thrombosis by DanlongCAPS
BAO Yu-long,DU Jia-lin,LI Xian-hua,GU Xiao-ying,ZHAO Lei,LIU He, CUI Ming-hao,SONG Da-fu
2010, 27(03): 19-21.
Asbtract
(
)
Related Articles
|
Metrics
Objective To study the action of DanlongCAPS for inhibiting thrombosis in Methods The inferior vena of the rat was ligated to make thrombosis in inferior vena,then the dry weight and humid weight of the internal clot was calculated; with the equipment of internal clot formation to observe and record the average blockage rate of the arteria carotis communis in 5 minutes of electric stimulation;the inhibiting effect of DanlongCAPS on adenosine diphosphate ( ADP ) induced by platelet aggregation was measured by platelet aggregation meter. Results DanlongCAPS could reduce the internal clot of the inferior vena,decrease the average blockage rate of the arteria carotis communis,and inhibite the platelet aggregation induced by adenosine diphosohate ( ADP) in the rabbit. Conclusion The results of the study confirm that the DanlongCAPS has the effect of inhibiting thrombosis.
The Effects of Heterogeneous Victoria A Acid Capsules on the Treatment of the Micro-acne on Rabbit Ear Induced by Acid and Staphylococcus epidermidis
ZHANG Hui-ying,CHEN He,LIU He,QIN Wen-yan,XIANG Shao-jie,TU Da-yu, GAN Yu,MENG Li,CUI Ming-hao,SONG Da-fu,BAO Yu-long
2010, 27(03): 22-24.
Asbtract
(
)
Related Articles
|
Metrics
Objective The effects of Heterogeneous Victoria A Acid Capsules on the treatment of the micro- acne on rabbit ear induced by oleic acid and Staphylococcus epidermidis. Methods Rabbit model of the formation of acne with spreading oleic acid to the ear,and subcutaneous injection of Staphylococcus epidermidis,was established,and then the model was treated with Heterogeneous victoria a acid capsules. Results The Heterogeneous Victoria A Acid Capsules markedly decreased sebaceous acid capsule diameter,and hair follicle size. Conclusion The Heterogeneous Victoria A Acid Capsules have a therapeutic effect to the micro-acne on rabbit ear.
Observation of Effect of Minipig Dorsal Paired Expanded Flap Model
LI Wei-wei,LIU Zhi-fei,QIAO Qun,WANG Xiao-jun,ZHU Lin,ZENG Ang, ZHANG Hai-lin,BAI Ming,FENG Rui
2010, 27(03): 25-28.
Asbtract
(
)
Related Articles
|
Metrics
Objective To observe the effect and safety of minipig dorsal paired expanded flap model. Methods 6 minipigs with weight 10 ～ 12 kg were chosen. And 6 symmetrical adjoining area (7 cm × 5 cm each) were drawn on the minipig's dorsum. Soft tissue expanders(100 mL) were buried in the left area,and the right area were control group. Results The survival rate in the expanded flap group was statistically different with the control group(P < 0. 05). The survival rate within the expanded flaps group was not statistically different ( P > 0. 05). Conclusion The minipig dorsal paired expanded flap model shows lower difference in expanded flap survival rate than control group. This is a suitable model for expanded flap survival research.
How to Ethics Thinking of Laboratory Animals in Biomedical Research
YANG Guo-bin
2010, 27(03): 29-31.
Asbtract
(
)
Related Articles
|
Metrics
Animal experiments is a indispensable measure in life science research,which plays a very important role in the development of biological medicine. But with the development of society,advance of science technology and improvement of the people morality level,ethical issue of animal experiments draws extensive attention of people. In this paper,the argument of animal experiments ethical issues,manner of medical word and the public at animal experiments were analysed,and the problem how to take animal experiments scientifically,reasonably and humanly to accept challenge suitably with animal experiment ethics were discussed. It is emphasized that the biomedical research should be coordinately developed with animal experiment ethics,which can make animal experiments fully serve science and technology advance,mankind health and society development.
Establishment and Implementation of SOPs for the Laboratory Animals Production Management in Barrier System
WANG Jin-heng,FAN Chang-fa,LIU Zuo-min
2010, 27(03): 32-33.
Asbtract
(
)
Related Articles
|
Metrics
SOPs for the laboratory animal production management were established and implemented based on the actual situation of barrier system,so as to realize standardzation managements,to ensure the quality of the laboratory animal,and to promote the management levels.
Analysis of Talents Construction of Management of SPF Animal Laboratory
XIE Ling-ling1,ZOU Yi-hai1,LIU Zhong-hua2
2010, 27(03): 34-35.
Asbtract
(
)
Related Articles
|
Metrics
The SPF animal laboratory,has been bearing the task of research and teaching of our University. The talents construction is the important part in management of SPF animal laboratory. It has Achieved some success for many years,which providing effective support for research and teaching.
The Application of Radio Frequency Identification(RFID) in the Management of SPF Laboratory Animals
WEN Ze-feng1,XIA Long1,QI Chang-yong1,HOU Dao-rong1,JIANG Ping2
2010, 27(03): 36-38.
Asbtract
(
)
Related Articles
|
Metrics
By using the principle of radio frequency identification and electronic labels,combined with the importance of reorganization and tracks for laboratory animals,explained the application of radio frequency identification and electronic labels in breeding and management of laboratory animals.
New Introduction Macaque’s Quarantine,Isolation and Raising Management
HUA Xiu-chun,SHI Yan-sheng,SUN Zhao-zeng,GENG Zhi-xian,KONG De-qiang,ZENG Lin
2010, 27(03): 43-46.
Asbtract
(
)
Related Articles
|
Metrics
When the macaque enters the new raising enviroment,they must adapt the enviroment process gradually. This progress should be completed by good work at quaranting,isolating and raising managemennt to reduce the rate of disease and death. Through this introduction work,the scientific method and the practical management has been built. The experience is useful for the simillar work.
Developmental Regulation of Thymus Organogenesis
LI Lan-lan,ZHANG Lian-jun,ZHAO Yong
2010, 27(03): 50-54.
Asbtract
(
)
Related Articles
|
Metrics
Thymus provides the complex microenvironment which is crucially required for T-cell development and establishing the immune self-tolerance of T cells. Thymic epithelial cells (TECs),which are composed of cortical and medullary TECs and arise from the third pharyngeal pouch,play an important role for this tightly regulated process of T cell development and maturation. Studies on the formation of a 3-dimensional thymic construction, migration of thymocytes and their precursors,and molecular events involving in thymus organogenesis will contribute better understanding the process of thymus development and offer important insights for preventing /treating thymusrelevant immune disorders.
The Research Progress on the Syntheses of Fullerene Biomolecule Derivatives
KANG Feng1,LU Sheng-ming2,HU Jian-wu2
2010, 27(03): 55-59.
Asbtract
(
)
Related Articles
|
Metrics
The important strategy of improving fullerene’s water solubility is the syntheses of its biomolecule derivatives. In this review,some syntheses of fullerene biomolecule derivatives are described. In addition,the study prospects on these derivatives are also included.
Serum Hormone Evaluation of Cyclomastopathy Model of Stagnation of Hepatic Qi in Multiparous Rats
WU Shu-guang1,WU Pei-xin2,WANG Ming-zhen1,ZHANG Shu-sen1,CHEN Zhen-zhen1, LI Shu-chai1,QIAN Ning1
2010, 27(03): 61-62.
Asbtract
(
)
Related Articles
|
Metrics
Objective To observe the serum hormone changes in multiparous rats with cyclomastopathy model of combination of disease and syndrome. Methods 20 female multiparous rats were divided into normal group and model group. Estradiol benzoate was given to the rats in the model group by intramuscular injection (1. 0 mg /kg) for 10 times, one time every three days. Then, the Progesterone ( 5. 0 mg /kg ) was injected for 5 days continuously. In addition,the rats were bound during the night for 15 times in the model group. The day after the final injection,foods were banded for 12 hours. Then blood was taken through femoral artery for further test after Sumianxin anesthesia. Results In model rats group,high-leveled prolactin and estrodiol accompanied with low progesterone were found in blood serum. Conclusion High-leveled serum estradiol is the major characteristic of cyclomastopathy model of combination of disease and syndrome in multiparous rats.
Construction and Application of Sterilization Equipment of Drinking Water for Laboratory Animals
WANG Long,LI Hong-cai,LU Sheng-ming
2010, 27(03): 63-64.
Asbtract
(
)
Related Articles
|
Metrics
Controlling the quality of laboratory animals drinking water is a key step for the safeness of laboratory animals breeding,The quality of water is to ensure the safety of laboratory animal breeding environment,therefore, construction and application of sterilization equipment of drinking water for laboratory animals could meet aseptic sterilization of drinking water standards.
