Abstract: Objective To observe the serum hormone changes in multiparous rats with cyclomastopathy model of combination of disease and syndrome. Methods 20 female multiparous rats were divided into normal group and model group. Estradiol benzoate was given to the rats in the model group by intramuscular injection (1. 0 mg /kg) for 10 times, one time every three days. Then, the Progesterone ( 5. 0 mg /kg ) was injected for 5 days continuously. In addition,the rats were bound during the night for 15 times in the model group. The day after the final injection,foods were banded for 12 hours. Then blood was taken through femoral artery for further test after Sumianxin anesthesia. Results In model rats group,high-leveled prolactin and estrodiol accompanied with low progesterone were found in blood serum. Conclusion High-leveled serum estradiol is the major characteristic of cyclomastopathy model of combination of disease and syndrome in multiparous rats.

Key words: SD rat, Multiparity, Cyclomastopathy, Stagnation of hepatic qi, Hormone

摘要： 目的观察经产大鼠乳腺增生病病证结合模型血清激素变化。方法将20只雌性经产SD大鼠分为正常组、模型组,模型组大鼠隔2日肌肉注射苯甲酸雌二醇注射液1.0mg/kg,连续10次,之后每日肌肉注射黄体酮5.0mg/kg,连续5日,在整个造模过程中隔日一次将其装入特制的束缚笼中束缚固定过夜,共15次。在末次注射黄体酮次日禁食12h,速眠新麻醉股动脉取血备检。结果模型组大鼠血清泌乳素、雌二醇升高,孕酮降低。结论经产大鼠肝郁气滞型乳腺增生病模型血清激素变化以血清雌二醇升高为主要特征。

关键词: SD大鼠, 经产, 乳腺增生病, 肝郁气滞, 激素