Table of Content

29 June 2013, Volume 30 Issue 02

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Correlative Study Between the Exterior Signs of Mice Model Induced by Thyroxine and Hyperactivity of Fire due to Yin Deficiency Syndrome

HE Yu-Wei, CHAI Cheng-Zhi, KOU Jun-Ping, QI Jin, YU Bo-Yang

2013, 30(02):  1-6. 

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Abstract: Objective To observe the correlation between the exterior signs of mice model induced by thyroxine and hyperactivity of fire due to yin deficiency syndrome to provide reference for constructing hyperactivity of fire due to yin deficiency syndrome animal model． Method 48 standard weight mice were randomly divided into control group and three model groups，12 each group，male and female in half． Model groups were managed with thyroid tablet suspension of different doses ( 40 mg / kg，80 mg / kg and 160 mg / kg) respectively，whereas the control group with double-distilled water． The body weight，food intake，water intake，body temperature，spontaneous activity and heart rate were measured dynamically． Result As compared with the control group，mice in the model groups manifested slowly increased body weight，enhanced food intake and water intake，elevated body temperature，quicken heart rate and frequent spontaneous activity，which could simulate the major clinical characteristics of hyperactivity of fire due to yin deficiency syndrome，such as emaciation，voracious appetite，thirsty for water， internal heat，palpitation，impatience，et al． Among which，the exterior signs of female mice in high-dose model group were extremely notable compared with the control group ( P ＜ 0. 01 ) ． Conclusion The exterior signs induced by thyroxine can simulate the major clinical characteristics of hyperactivity of fire due to yin deficiency syndrome，the correlation between the exterior signs of female mice model induced by thyroxine i． g． 160 mg / kg / d for consecutive 14 days and hyperactivity of fire due to yin deficiency syndrome is more significantly．

Survival Test and Effect of Cyclophosphamide in H22 Solid Tumor-bearing Mice

RAO Zi-Liang, ZHENG Jia-Lin, HUANG Wei, WANG Nuo, ZHONG Zhi-Yong, KUANG Shao-Song, TANG Xiao-Jiang

2013, 30(02):  7-10. 

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Abstract: Objective To study Survival time and Effect of Cyclophosphamide in H22 solid Tumor-bearing mice．Method H22 cells were implanted into the left axilla of 50 BALB / c mice． Tumour growth was observed． 5 days later，The mice were randomly divided into Cyclophosphamide group and the control group by tumor volume． Cyclophosphamide group intraperitoneal injection of cyclophosphamide 50mg / kg，2 times a week． Observe the general clinical manifestations， tumor formation time，the rate of tumor formation，weight，food intake，water intake，tumor volume and survival time． Result The H22 tumour tubercles could be formed on day 5． The tumour-take rate was 100% ( 50 /50) ． The average survival time of H22 subcutaneous xenograft mice is 87 days． The average survival time of Cyclophosphamide group is 113days ( Life extension rate is 30. 3%，P ＜ 0. 01) ． Conclusion H22 ascites hepatoma transplanted subcutaneously in mice is an ideal model of solid tumors，which have normal immune function，and can be used for the survival test of biological response modifier anticancer drugs．

Biodistribution of SUV and CT Housefield Unit in 18F-FDG PET /CTImaging at Various Normal Organs of SD Rat

ZHANG Wei-Tao, HUO Tian-Long, AN Bei, YAO Wei, YI Xian-Feng

2013, 30(02):  11-13. 

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Abstract: Objective To define the standardized uptake value ( SUV) and CT housefield unit in various normal organs of SD rat during 18 F-FDG PET /CT scanning． Method Drew the regions of interest ( ROI) on parenchymal organs in SD rats and analyzed the data of SUV and CT housefield unit． Result The SUV and CT housfield unit of normal organs in SD rats were correlated with their physiological and structure characteristics． Conclusion The results provided the reference for evaluation and semi quantify analysis in PET /CT imaging of SD rats．  

Reference Value for Body Weight、Mortality、Hematology and Serum Biochemistry of SD Rats in the Combined Chronic Toxicity / Carcinogenicity Study

ZHONG Zhi-Yong, LIU Sheng-Lai, ZHENG Gui-Lan, ZHAO Shi-Hai, XIE Chao-Min, WANG Gang, HUANG Jian-Xun, TANG Xiao-Jiang

2013, 30(02):  14-19. 

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Abstract: Objective To investigate the change of reference value for body weight、mortality、hematology and serum biochemistry of SD rats in test method of combined chronic toxicity / carcinogenicity study． Method 135 male and 138 female specific pathogen free SD rat of 4 weeks were selected． We measured the body weights once a week between 4 ～ 17 weeks of age and once four week between 18 ～ 108 weeks of age． The indexes of hematology and biochemistry of SD rats were measured at the age of 8 weeks old、30 weeks old、56 weeks old、82 weeks old、108 weeks old and record the mortality rate every day． Result The body weight of male rats was much higher than female rats from 6 weeks old． The 82 ～ 108 weeks of age was the rapid growth of mortality in rats． The coefficient of variation of indexes of hematology and biochemistry of male and female become larger with increasing age． Gender has a significant impact on hematology and serum biochemistry in rats． But the hematology and serum biochemistry which has significant difference between female and male become less with increasing age． Conclusion During the safety evaluation tests，expecially combined chronic toxicity / carcinogenicity study，both of gender and age have a greater impact on the body weight、hematology and serum biochemistry． Male and female should be separated from the statistics and the animals background data should be used at the same weeks．

Study on the Anti-inflammatory Effect of Lumbago Plaster

BAO Yu-Long, DU Jia-Lin, FAN Ying-Lan, GAN Yu, LI Xian-Hua

2013, 30(02):  20-22. 

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Abstract: Objective Study on the effect of lumbago plaster anti-inflammatory action． Method Through determining prostaglandin E2 and histamine in local tissue around the nerve root of lumbar intervertebral disc protrusion model rat，and researching anti-inflammatory effect by using rat agar granuloma and ear swelling of mice induced by xylene． Result ( 1) compared with the model group，prostaglandin E2 content in the middle dose and the high dose group decreased significantly，histamine content in the low group，middle group and the high group decreased obviously，the difference was significant． ( 2) compared with the control group，the weight of granuloma in the low，middle and the high dose group decreases obviously，the difference was significant． ( 3) compared with the control group，ear swelling in the low，middle and the high dose group decreases obviously，the difference was significant Conclusion lumbago plaster can significantly reduce nerve root local inflammatory mediators，it also

Microsatellite Marker of Early Warning Experiment Research on Genotoxicity of Phenol in Red Crucian Carp C1HD

ZHU Xiao-Juan, DONG Xian-Hui, LIAN Gao-Jian, WU Duan-Sheng

2013, 30(02):  23-31. 

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Abstract: Objective To study the microsatellite markers of early warning on the response of genotoxicity of phenol in red crucian carp C1HD． Method The red crucian carp C1HD were divided into 4 exposure groups and 1 control group and choose 5 microsatellite markers to study the effect of phenol on the microsatellite polymorphism after exposed to different concentration phenol for 8 d． Result The indexes were increased by the enrichment of phenol in red crucian carp C1HD，such as the gene heterozygosity ( H) ，gene polymorphism content ( PIC) ，and so on． In the 8th day，the gene heterozygosity ( H = 0. 13 ± 0. 19) reached the peak value after exposed to thephenol of 1 /2 LC50 And in the 6th day，the gene polymorphism content( PIC = 0. 42) reached the peak value after exposed to the phenol of 1 /8 LC50 ． Conclusion The microsatellite polymorphism will be changed in the red crucian carp C1HD after phenol was enriched to a certain concentration． The microsatellite heterozygosity ( H) and polymorphism information content ( PIC) will be increased with the enrichment of phenol and the extension of exposure time．

Relative Factors for Experimental Atherosclerotic Plaque of Rabbit Induced by Immunolesion and High Fat Diet

LIU Jian-Gang, DONG Guo-Ju, MA Lu-Bo, SHI Da-Zhuo

2013, 30(02):  32-38. 

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Abstract: Objective To investigate the relative factors for experimental atherosclerotic plaque of rabbit induced by immunolesion and high fat diet． Method 30 Japanese white rabbits，intravenously injected bovine serum albumin ( 250mg / kg) ，feeding high fat diet after injection． 7 days later，injected again bovine serum albumin ( 250mg / kg) ． Meanwhile，established purely high fat diet group and normal diet group as control，10 rabbits each group． 72 days later，got blood from central artery of rabbit ears to determine lipid level，inflammation factors，vasoactive substance，platelet aggregation and vascular endothelium，etc． and analyzed which related to the pathological change of atherosclerotic plaque or not with SAS statistic software． Result After 72 days，the aorta of both the test group and the purely high fat diet group formed plaque． The plaque of the test group is localized，which is similar to the human being． The lipid level and the content of inflammatory factors，e． g． ，interleukin － 6 ( IL － 6 ) ， interleukin － 8 ( IL － 8 ) and high sensitivity-C reactive protein ( hs-CRP) are obviously higher than the control group; the content of vasoactive substance and thromboxane B2( TXB2) changed significantly than the control group ( P ＜ 0. 05) ． By the Pearson correlation coefficient statistics processing，And above factor into positive correlation to the plaque size induced by immunolesion and high fat diet; high density lipoprotein ( HDL-C，r = － 0. 58 ) ) ， nitric oxide ( NO，r = － 0. 26) are negatively related with the plaque size，and the correlation coefficient of HDL-C is the greatest． Conclusion The rabbit atherosclerotic plaque induced by immunolesion and high fat diet formed in a short time with obvious pathological change，which is similar to that of human beings and closely related to lipid level，inflammation factor，platlet aggregation and endothelial factor，which further show it more reasonable for evaluation the atherosclerosis．

Pathology of White Muscle Disease in Weanling Rabbits

ZHOU Jie, ZHOU Chang-Jun, ZHAO Ya-Li, LIU Li-Min, ZHAO Xiao-Qing, ZHANG Jing-Xu, LIU Ji-Ming, YANG Shu-苹

2013, 30(02):  39-41. 

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Abstract: Objective To observe the pathological change in weanling rabbits with white muscle disease． Method Pathogenic microorganisms were isolated from weanling rabbits with white muscle disease and cultured． The rabbits were dissected，of which the skeletal muscle，cardiac muscle and spinal were isolated，fixed with 10% neutral formalin，prepared into routine tissue sections and observed for histopathology by HE and calcium staining． Result Generalized symmetric muscular dystrophy was observed in systemic skeletal muscle as well as partial cardiac muscle and smooth muscle． Bleeding was observed in subcutaneous tissue，skeletal muscle and spinal canal of four rabbits of which two were complicated with coccidial enteritis． Conclusion The disease in rabbit was diagnosed as white muscle disease，of which the main pathological characters included generalized denaturation and coagulative necrosis of systemic skeletal muscle and proliferation of interstitial connective tissue．

A Clean Bench Transfer Equipment with Horizontal Flow

WANG Long, YIN Liang-Hong, BAI Yu, HU Jian-Wu, LIU Yan

2013, 30(02):  42-44. 

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Abstract: To develop new clean bench transfer equipment with horizontal flow，we connected to isolator and the new clean bench transfer equipment． In the standard isolate environment，we could realize to select animals， weighing，packaging and other treatment． safe operation， convenient， fast， and greatly improving the work efficiency，reduce loss and waste of animals，and to avoid the risk of contamination of the isolator．

Emphasis in the Process of Strain Selection of Beagle Dogs

LIAN Xue-Ke, LIU Yun-Zhong, CHEN Zhao-Wei, ZHANG Dun-Wei, LI Ming-Tao, LIN Meng-Yi

2013, 30(02):  45-47. 

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Abstract: Strain selection scientifically is important guarantee for maintaining beagle dogs’genetic stability． Referencing to the AKC standard and combining with the current situation of domestic Beagle dog breeding center， this article aims to discuss problems that we face to when selecting the parental Beagle dogs from perspectives such as appearance signs，Physical signs ，personality，genetic signs，etc．

Development of Application of Tree Shrew in Human Disease Animal Models Research

HUANG Xiao-Yan, XU Juan, SUN Xiao-Mei, DAI Jie-Jie

2013, 30(02):  59-65. 

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Abstract: Tree shrew is a newly developed animal model in medical experimental research and many of its biological characteristics similar to human，The animals have been infected with various human pathogenic viruses， and has been widely used in research in bacterial infectious disease，pneumatology，endocrinology ，neurology and oncology． This paper aims to review the recent progress of application of the tree shrew as animal model in human disease research．