Laboratory Animal Science

Study on Anatomy for the Hedgehog( Erinaceus europaeus) (Ⅱ)-Skeleton

Deng Wen\ Pang Youzhi\ Zong Liuxiang et al Luo Yang Agricultural College

2000, 17(01): 0-0.

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In this experiment, 28 skeleton samples from the adult hedgehogs (Erinaceus europaeus) (♀16,♂12)were studied anatomically.There are 352～353 skeleton bones of the hedgehog including 48 skull skeletons,43～44 vertebrae,(The vertebral formula is C 7T 15 L 6S 3C y12-13 ),15 pairs of ribs,5 sternebrae,47 bones in each fore limb,48 bones in each hind limb,and 36 teeth,[The dental formula is 2(I32,C11,P32,M33)=36].There is no baculum in the hedgehog.There are some sexual differences in the hedgehog skeleton and some traits adaptable to the habits and characteristics of the hedgehog.

Comparision of Protein Distribution in Different S180 Strains with Electrophoresis and Immunohistochemistry

Wang Xiufang\ Lu Zhanjun\ Liu Fuying Department of Laboratory Animal Science,Hebei Medical University,Shijiazhuang,050017$$$$〔

2000, 17(01): 0-0.

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To study the protein distribution in different S180 Strains using SDS polyacrylamide gel electrophoresis (SDS-PAGE) and immunohistochemistry were used.The number of protein bands of Sarcoma 180 strain from The Beijing Cancer Institute was the most and Wuhan University's was the least.The protein distribution of Raf\|1,TrkA,Gai,Gao and NF k Bp65 were observed in different S180 strains with immunohistochemistry.The positive rate of TrkA,Gai and Raf\|1 was less than 0.2%.Positive rate of Gao was 4.2%. The positive rates of NF k Bp65 were 76.0%,23.1%,4.5% and 17% respectively.Chitest showed significantly difference (P<0.01) excepting between S180 strains from Institute of Pharmacology of Chinese Medical Academy and those from Beijing Cancer Institute.The results indicate that some protein compositions of the Sarcoma 180 cells from 4 institutes not only have quantitative,but also have qualitative differences.Therefore the quality of S180 cell strain should be controlled.

The Pharmacokinetic Study of 5-Fu Implant Administrated Via Subcutaneous Embedding

Huang Dewu 1\ Song Biwei 2\ Sun Xin 3 et al Anhui Medical University,

2000, 17(01): 0-0.

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Objective:To investigate the pharmacokinetics of 5-Fu implant (5-FuI) in dogs.Methods:The 5-FuI concentration in plasma were detected with HPLC.Results:It was found that the characteristics of the plasma drug concentration\|time curve fit the one compartment open model with sustained release preparation after subcutaneous embedding of 5-FuI 20mg.kg -1 to dogs.The sustained release half\|life time (T 0.5 r)was as long as 52.7±10.9hrs.The 5-Fu concentration in plasma was higher than the effective anticancer concentration (1 μg.mL -1 ) for 216～240hrs.Conclusions:5-Ful embedded subcutaneously has markedly sustained releasing effect,5-FuI is a new dosage form for 5-Fu with excellently pharmacokinetic characteristics.

The Influence of Rabbit Vaccination on the Results of Drug Pyrogen Checking

Pan Shanqing Chen Ziyuan Li Yuzhang Zhang Zudang Hunan Institute of Drug Control

2000, 17(01): 0-0.

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The purpose of the study was to expore the influencing factors on the sensitivity of vaccinated rabbit to bacterial endotoxins as drug pyrogen. The resuts showed that the rabbits vaccination did not increase the rabbit temperature after giving bacterial endotoxin, but the deviation of the change of temperature in rabbits from countryside was greater than that of the experimental one.

Study on Toxicity of Shukangning Gel by Vaginal Administration in Rat

Zhang Genglun\ Hou Chuanxiang\ Wang Lijuan Shandong Institute for Drug Control,

2000, 17(01): 0-0.

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This experiment showed that Shukangning gel had no obvious side\|effects, no death on the female rats and no signs of congestion, oedema and tissue pathological changes on the vaginal mucosa in acute toxicity by vaginal administration with a total dose of 10.95g crude drug per kg, which was 109.5 times of a clinical dose per 60kg. Whereas single and multiple administration of the drug did not significantly stimulate the vaginal mucosa. There was no significant difference in the body weight, index of hematology and major blood biochemistry, relative organ weight and major organ pathological examination between the control and drug treated groups in long term toxicity with a high dosage of 4.38g per kg which was 43.8 times of a clinical dose per 60kg and low dose of 2.19g per kg. The test had been continued for 3 weeks which was as 3 times long as that of a clinical use period.

Research on the Anti-oxidation of the Alcohol Extract of Pilose Antler

Chen Shuming\ Nie Xiangtin Shanxi Agricultural University

2000, 17(01): 0-0.

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In this paper,the effects of the alcohol extract of pilose antler(AEPA) on the mice treated by cyclophosphamide were studied.It was found that activity of SOD in the mice erythrocyte that with AEPA could significantly increase with AEPA (p<0.05), and the content of MDA in the mice decreased significantly (p<0.05) .The results showed that AEPA could enhance the mice's anti\|oxidation.

Effect of Chongcao Yangshen\% Capsule on Immune System of Mice

Zhang Jinsong\ Li Junan Zhejiang Institute for Drug Control

2000, 17(01): 0-0.

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Oral \%Chongcao Yangshen\% Capsule (CYC) causeda significant decrease of the weights of thymus of mice, and enhanced the delayed hypersensitive reaction in mice. The results showed that CYC raised the clearance rate of iv charcoal particles, and increased the value of HC\-\{50\} induced by cyclophosphamide in mice.

The Effect of BaoFeiTai on the Natural Killer Cells Activites with Lewis Lung Cancer Bearing Mice and its Chemotherapy

Li Xingqin\ Xu Zengnian\ Zhou Zunqin Hebei Academy of Medical Science\

2000, 17(01): 0-0.

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In this experiment, the effect of BaoFeiTai(BFT) was observed on the Natural Killer cells (NK) activities with Lewis lung cancer bearing mice and its chemotherapy. The results of the experiment showed that NK cells activities increased both in BFT group and in BFT+chemotherapy group obviously, as compared with the control group and with chemotherapy group (\%P\%<0.05) respectively. It suggested that the inhibitory effect of BFT on Lewis lung cancer cells might be achieved by way of strengthening the cellar immune function of the mice.

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