Abstract: Abstract: Objective To explore the feasibility of disinfection using an ozone generator in the animal lab equipped with individual ventilated cages ( IVC) ． Method Use ozone testing tube to test the ozone concentration in the animal lab and the working IVC，observe health condition，biochemical index，histology changes of the windpipe and lungs in rats． Result The low，medium and high filters on the IVC cage have a certain barrier function to prevent ozone，and can reduce 80% of ozone． Under no special protection，the rats have a high resistance to the ozone． In the 35 m3 IVC animal lab，using an ozone generator with 3 g /h ozone production for 35 minutes exposure，there are no detectable physiological and pathological changes． Conclusion Ozone generator can be used for environment disinfection in IVC animal laboratory．

Key words: ozone, experimental animal, individual ventilated cages ( IVC)

摘要： 摘要: 目的探讨独立通风系统( IVC) 动物实验室内使用臭氧发生器消毒的可行性。方法使用臭氧检测管测定 实验室内的和运行中的IVC 内的臭氧浓度，观察大鼠的健康状况、生化指标、气管和肺的组织病理学状况。 结果发现IVC 笼盒的低、中、高3 级过滤器，对臭氧有一定的屏障作用，可以减少80%。大鼠在无特殊保护的情 况下，对臭氧的耐受能力较高，在面积为35 m3 的IVC 动物实验室内，对采用臭氧发生量3 g /h 的设备开启35 min， 没有检测到大鼠生理和病理的变化。结论臭氧发生器能够用于IVC 动物实验室的环境消毒。

关键词: 臭氧, 实验动物, 独立通风系统