Abstract: Abstract: Objective To study the preparation and identification of the model of rabbit knee osteoarthritis with the improved operational method of Hulth． Method According to the improved operational method of Hulth，rabbits were cut off knee medial collateral ligament，anterior cruciate ligament，and medial meniscus． After 8 weeks， cartilage and synovium were observed by X ray photograph，naked eye and HE staining． The expression of IL － 1、 TNF － α in serum and joint fluids were detected by Enzyme-linked Immunosorbent Assay ( ELRSA) ． Result X ray showed that obvious arthrostenosis and spur were developed． Cartilage defect and synovial inflammation were observed by naked eye． HE staining showed that cartilage defected，chondrocyte reduced and apoptosis． Compared with normal group，There were higher expression of IL － 1、TNF － αin serum and joint fluids in model group( P ＜ 0. 05) ． Conclusion After 8 weeks，the model of rabbit knee osteoarthritis with the improved operational method of Hulth was successfully made． And the model can accurately reflect the characteristic of osteoarthritis through the image analysis and biochemistry detection． It will be the theoretical basis and research groundwork for clinical treatment．

Key words: rabbit knee osteoarthritis, the improved operational method of Hulth, preparation, dentification

摘要： 摘要: 目的探讨改良Hulth 法制备兔膝骨关节炎模型及鉴定方法。方法改良Hulth 法将兔膝内侧副韧带及前 交叉韧带切断，内侧半月板切除，造模8 周后拍片，将兔处死对关节腔软骨及滑膜大体观察; HE 染色以及ELISA 检 查血清及关节液中IL － 1、TNF － α 的表达。结果X 线显示有明显的关节腔狭窄及骨刺形成。大体观察软骨有缺 损，滑膜炎症形成。HE 染色软骨缺损，软骨细胞分散、减少，形态不稳定。ELISA 检查血清及关节液中IL － 1、TNF － α 的表达增高( P ＜ 0. 05) 。结论改良Hulth 法制备的兔膝骨关节炎模型通过影像及生化指标检查，能准确反映 骨关节炎特征性表现，为相关基础研究及临床治疗提供了理论依据。

关键词: 兔膝骨关节炎, 改良Hulth 法, 制备, 鉴定