Abstract: Abstract: Objective Establish mouse superovulation and embryo transfer technology，in order to further use the technology to control their offspring，and gradually will these technologies applied to other animals，so as to speed up its breed improvement，improve the utilization rate of good varieties and individual． Method Of 4-week-old C57BL /6 female mouse by intraperitoneal injection 7. 5 IU PMSG ( pregnant mare serum gonadotropin) 48 hours after intraperitoneal injection of superovulation 7. 5 IU HCG ( human chorionic gonadotropin) and C57BL /6 male rats mated，see bolt 0. 5 days，1. 5 days and 3. 5 days，remove the corresponding embryo，the resulting embryoscreening，elect better quality embryos and embryonic stages ( single cells，2 cells capsule fallopian tube or uterus KM pseudopregnant mice mouse embryo) into estrus synchronization see bolt after KM ligation male rats mating stimulus be in production pregnancy after transplantation in mouse． Result Superovulation 88 C57BL /6 mice，see bolt 62，a total of 510 single-cell embryos，400 2-cell stage embryos，blastocysts 35，the ministry of tubal transplantation，tubal notches，uterine transplant transplant pseudopregnant female mice 43，pregnancy producing 39 farrowing 361． Conclusion Tubal fimbria transplantation compared with tubal Jiankou transplant was no significant difference tubal transplantation hurt small animals，but for patients who require a higher; fallopian tube the Jiankou transplant operation is relatively quick，but the larger animal injury; uterine transplant the pregnancy best effect at this stage of embryo easily implantation．

Key words: mouse, super ovulation, embryo transfer

摘要： 摘要: 目的建立小鼠胚胎移植技术，探索小鼠胚胎输卵管伞部移植、输卵管剪口移植和子宫移植技术要点，为进 一步将该项技术应用到生物净化等领域提供参考数据。方法对4 周龄C57BL/6 雌性小鼠采用腹腔注射7. 5 IU PMSG( 孕马血清促性腺激素) ，48 h 后腹腔注射7. 5 IU HCG( 人绒毛膜促性腺激素) 进行超数排卵处理，并与 C57BL/6 雄鼠合笼，分别在见栓0. 5 d、1. 5 d 和3. 5 d 取出相应胚胎，对所得胚胎进行筛选，将质量较好的胚胎选 出，并将相应胚胎阶段( 单细胞、2 细胞、囊胚) 移入同期发情并经KM 结扎雄鼠交配刺激后见栓的KM 假孕小鼠输 卵管或子宫内，待妊娠生产后得到移植小鼠。结果对88 只4 周龄雌性C57BL/6 小鼠进行超数排卵处理，见栓62 只，共取得单细胞胚胎510 枚、2 细胞期胚胎400 枚、囊胚35 枚，采用输卵管伞部移植、输卵管剪口和子宫移植，共 移植假孕母鼠43 只，妊娠生产39 只，产仔361 只。结论输卵管伞部移植与输卵管剪口移植比较无显著差异，输 卵管伞部移植对动物伤害较小，但对术者要求较高; 输卵管剪口移植操作较快捷，但对动物损伤较大; 子宫移植的 妊娠效果最好，处于这一阶段的胚胎极易着床。

关键词: 小鼠, 超数排卵, 胚胎移植