Abstract: By observing the changes in learning and memory abilities in various behavioralexperiments of the para-chlorophenylalanine ( PCPA) insomnia rat model within a week after successfulmodeling, the stability of the PCPA insomnia model was verified. Method Thirty SD rats were randomlydivided into blank group and model group, the rats in the blank group were fed normally, andestablishment of insomnia model by intraperitoneal injection of PCPA into model group rats. Fivebehavioral tests including Morris water maze test were performed in the first week after modeling.Result Compared with the blank group, the sleep latency of the model group was significantly prolonged(P < 0. 01 ) , and the sleep duration was significantly shortened ( P < 0. 01 ) . In the water mazeexperiment, the time of seeking platform in the model group was prolonged ( P < 0. 01) . In the Barnesmaze experiment, the total residence time at the target portal of the model group rats during the testexploration period was significantly shortened (P<0. 01) . In the dark avoidance experiment, the numberof errors in the model group increased ( P < 0. 05) , and the latency was significantly prolonged ( P <0. 01) . In the elevated cross maze experiment, the open arm residence time of rats in the model group实验动物科学 41 卷was significantly shortened ( P < 0. 01 ) . In the open-field experiment, the dwell time, the number ofuprightness and the total distance of movement in the central area of the model group were all lower thanthose of the blank group, among which the dwell time in the central area was significantly different (P<0. 01) . Conclusion The PCPA insomnia model has high reproducibility and stability, and can be usedas a basic model for sleep and memory-related studies.

Key words: PCPA, insomnia model, behavioural evaluation

摘要： 目的 通过观察对氯苯丙氨酸( PCPA)失眠大鼠模型在造模成功后一周内,各类行为学实验中的学习记忆能力改变,验证 PCPA 失眠模型稳定性。 方法 30 只 SD 大鼠随机均分为空白组和模型组,空白组正常饲养,对模型组大鼠腹腔注射 PCPA 建立失眠模型,建模后第 1 周进行 Morris 水迷宫实验等 5 项大鼠行为学监测。 结果 与空白组比较,模型组大鼠睡眠潜伏期显著延长(P<0. 01) ,睡眠持续时间显著缩短(P<0. 01) ;在水迷宫实验中,模型组大鼠寻台时间延长(P<0. 01) ;巴恩斯迷宫实验中,模型组大鼠测试探索期的目标洞口停留总时间显著缩短( P<0. 01) ;避暗实验中,模型组错误次数增多( P< 0. 05) ,且潜伏期显著延长( P< 0. 01) ;高架十字迷宫实验中,模型组大鼠开臂停留时间显著缩短(P<0. 01) ;旷场实验显示模型组大鼠在中央区域的停留时间、直立次数和运动总距离均低于空白组,其中中央区域的停留时间具有显著差异(P<0. 01) 。 结论 PCPA 失眠模型具有较高的可重复性和稳定性,可以作为睡眠和记忆相关研究的基础模型。

关键词:  , PCPA, 失眠模型, 行为学评价