Abstract: Objective We summarized and analyzed the scientific thinking and related treatment methodcaused by occasional sick large animals in scientific research to provide practical reference to handle thesimilar conditions. Method In a study of techniques related to laparoscopic porcine livertransplantation, the liver donor pig developed a sudden respiratory cardiac arrest after routine anesthesiavia the marginal ear vein. Therefore, the cyst wall cells were cultured and the cyst wall tissues wereexamined pathologically. Result The cyst wall cells showed a grape tandem suspension growth. The smear staining result showed that the cultured cyst wall cells were mainly inflammatory cells. The cystwall tissue was mainly composed of granulation tissue and fibrous tissue, which was a chronicinflammatory hyperplasia. Conclusion The following enlightenments can be obtained from the occasionalencounter of this sick pig: ①routine animal health examination should be performed before large animalexperiments; ②researchers should have a high sensitivity to infectious disease protection; ③researchersshould maintain a highly scientific sensitivity to the occassional situations during the experiment;④comprehensive pathological samples should be retained during the experiment; ⑤ the monitoring ofanimal health status in the farm should be improved

Key words: liver transplantation, diseased animals, scientific thinking, treatment methods

摘要： 目的 分析科学研究中偶遇患病大动物所引发的科学思考及相关处理方法,为类似状况的处理提供实践参考。 方法 在腹腔镜猪肝移植相关技术的研究中,供肝动物猪经耳缘静脉常规麻醉后,出现呼吸心脏骤停。 尸体解剖后发现腹腔囊性 包 块 和 大 量 腹 水 以 及 心 包 腔 积 液,对 包 块 囊 壁 细 胞 进 行 培 养 及 囊 壁 组 织 进 行 病 理 检 查。结果 囊壁细胞培养呈葡萄串状悬浮生长,涂片染色表明所培养细胞主要为炎性细胞。 HE 染色显示囊壁主要由肉芽组织及纤维组织构成,为慢性炎性增生所致。 结论 偶遇此例患病动物可得到以下启示:①大动物实验前需进行常规动物健康检查;②科研人员应具备高度的传染病防护敏感性;③科研人员在实验过程中需保持科研敏感性;④实验过程中应保留全面的病理标本;⑤养殖场质检人员须提升对动物的健康状况监测。

关键词:  , 肝移植, 患病动物, 科学思考, 处理方法