Abstract: Objective To compare the effects of two dehydration procedures and five wax immersion timeson the quality of paraffin sections of mouse liver and intestinal tissues, with a view to optimizing thepreparation procedures of paraffin sections. Method The liver and intestinal tissues of BALB / c micewere prepared in two different dehydration procedures ( Procedure I and Procedure II) and five differentwax immersion procedures ( total immersion time of 90, 120, 150, 180 and 210 min) to compare theresult of tissue sectioning and HE staining. Result The paraffin sections produced by dehydrationprocedure 1 were easily fragmented, with obvious wrinkles and cavities in the center of the tissue; theparaffin sections produced by dehydration procedure 2 were intact and free of cavities, and the structureof the paraffin sections was observed to be intact and clear under the microscope after HE staining.Compared with the other four wax immersion procedures, the paraffin sections produced with a totalimmersion time of 150 min were the most complete and clear after staining. Conclusion The quality ofthe paraffin sections produced by using the dehydration procedure II and the total time of wax immersionof 150 min was improved and easy to observe under the microscope, which is recommended as theoptimized procedure for paraffin sections of mouse liver and intestinal tissues.

Key words: paraffin section, HE taining, mice liver, mice intestinal, dehydration, wax immersion

摘要： 目的 比较两种脱水程序和 5 种浸蜡时间对小鼠肝、肠道组织石蜡切片质量的影响。 方法 以 BALB / c 小鼠肝和肠道组织为材料,按照两种不同的脱水程序(程序一、程序二)和 5 种不同的浸蜡程序 (浸蜡总时间为 90、120、150、180 和 210 min) 制备石蜡切片,比较其组织切片和 HE 染色的效果。 结果 脱水程序一制作的石蜡切片容易碎裂,皱褶明显,组织中央易见空洞;脱水程序二制作的石蜡切片完整无空洞,石蜡切片经 HE 染色后镜下观察结构完整清晰。 相较于其他 4 种浸蜡程序,浸蜡总时间为 150 min 时制作的石蜡切片染色后观察最完整清晰。 结论使用脱水程序二及浸蜡总时间为 150 min 时所制作的石蜡切片质量有所提升,易于镜下观察,推荐作为小鼠肝和肠道组织石蜡切片的优化程序

关键词: 石蜡切片, HE 染色, 小鼠肝, 小鼠肠道, 脱水, 浸蜡