Abstract:

Abstract: Objective To analyze the intestinal microbiome composition and community structure of normal and hypobaric hypoxic environment in mice. Method The 6-week old SPF C57BL / 6 N male mice were randomly divided into 2 groups with 6 mice in each group: the normal atmospheric pressure group ( alt. 20 m to 60 m, oxygen concentration 21%) as the control test group ( the CT Group) , the hypobaric hypoxic group ( alt. 5 000 m, oxygen concentration 10%, the HYP group ) . After 4 weeks, the fresh feces of the mice were collected to investigated by 16 s sequencing analysis. Result The relative abundance of Bacteroidetes and Verrucomicrobia in the feces of mice in the HYP group was higher than that in the CT group, the relative abundance of Firmicutes and Proteobacteria was lower than the CT group. The heat map result show an increase in the OTUs of the Ruminococcus, Enterococcus, Akkermansia, Prevotellaceae UCG-001, Bacteroides, Alistipes, Parabacteroides and a decrease in Lactobacillus, Allobaculum, Ruminiclostridium and Roseburia in the HYP group. Compared with the CT group, the dominant flora is Akkermansia ( 20. 60%) of Verrucomicrobia. Conclusion The composition and community structure of intestinal microbiome is affected by the simulated conditions of altitude environment which may be related to the hypobaric hypoxic.

Key words:

Key words:hypobaric hypoxic, intestinal microbiota, bacterial community structural, 16 s rDNA sequence

摘要：

摘要:目的 探索模拟高原环境低压低氧条件对小鼠肠道微生物菌群的影响。 方法 将体质量均匀的 6 周龄 SPF级雄性成年 C57BL / 6 N 小鼠随机分为两组:常压常氧组和低压低氧组,每组 6 只,分别饲养于常压常氧(海拔 20 ~60 m、氧浓度约 21% ) 、低压低氧(模拟海拔 5 000 m、氧浓度约 10% )的环境中,实验周期为 4 周。 实验结束后,采集小鼠肠道粪便样品,提取 DNA,进行 16 s rDNA 高通量测序。 结果 在门级水平上,与常压常氧组比较,低压低氧组相对丰度较高的为拟杆菌门( Bacteroidetes)和疣微菌门( Verrucomicrobia) ,常压常氧组相对丰度较高的为厚壁菌门( Firmicutes) 、变形 菌 门 ( Proteobacteria) 等;在 属 级 水 平 上,低 压 低 氧 组 的 瘤 胃 球 菌 属 ( Ruminococcus) 、肠 球 菌 属( Enterococcus ) 、 阿 克 曼 菌 属 ( Akkermansia ) 、 普 雷 沃 氏 菌 UCG-001 属 ( Prevotellaceae UCG-001 ) 、 拟 杆 菌 属( Bacteroides) 、另枝菌属( Alistipes) 、狄氏副拟杆菌属( Parabacteroides) 等含量增加;乳杆菌属( Lactobacillus) 、别样棒菌属( Allobaculum) 、瘤胃梭菌属( Ruminiclostridium) 、罗斯氏菌属( Roseburia)等含量降低。其中,低压低氧组中隶属于 Verrucomicrobia 的 Akkermansia 升高的最为明显,占总含量的 20. 60% 。 结论 模拟高原环境下,小鼠肠道微生物菌群发生变化,可能与模拟高原环境中氧气含量较低且大气压较低有关。

关键词:

关键词:低压低氧条件, 肠道微生物菌群, 菌群结构, 16 s rDNA 测序