Abstract:

Abstract: Objecive To observe the effects of XiaoChaihu KeLi combined with Levofloxacin on Escherichia coli ( E. coli) result on antibacterial and antidiarrhea in vivo. Method The seventy Kunming mice (weighing (20±2) g, sex in half) were divided into seven groups, and different concentrations of E. coli bacterial were intraperitoneal injection, which to determine the concentration of bacterial suspension of minimum lethal dose ( MLD) . The fifty Kunming mice were divided into five groups, which were model group, normal saline group, levofloxacin group, Xiaochaihu granule group and combined treatment group ( levofloxacin and Xiaochaihu granule ) . The bacterial suspension concentration of minimum lethal dose ( MLD) in mice was determined. In the in vivo antibacterial test, the bacterial diarrhea model of mice was established by intraperitoneal injection of E. coli suspension ( MLD concentration) . After 15 days of intragastric administration of corresponding drugs, the protective effect of combined drugs on lethal E. coli infection in mice was explored. The effects of combined administration on diarrhea index and organs of mice infected with E. coli and on intestinal propulsion rate of mice were explored after 7 days of gavage of corresponding drugs. Result The combined drug had a good protective effect on diarrhea induced by E. coli infection in mice, and the survival rate was 90%, which was statistically significant compared with the levofloxacin group and the Xiaochaihu granule group ( P<0. 05) . Combined drug group significantly improved the diarrhea symptoms of mice, compared with model group and Xiaochaihu granules group, diarrhea index of combined drug group had significant statistical significance ( P<0. 01) . Compared with the levofloxacin group, the diarrhea index of the combined treatment group was statistically significant ( P <0. 05) . The combined drug group protected the organs of mice and reduced the effect of diarrhea caused by E. coli infection on the toxicity of the organs, it could also improve the immune organ coefficient and improve the body immunity. Compared with model group, blank group, levofloxacin group and Xiaochaihu granule group, the thymus organ coefficient and spleen organ coefficient of combined medication were statistically significant ( P<0. 05) . Compared with levofloxacin group and model group, the liver organ coefficient and kidney organ coefficient in the combined treatment group were statistically significant ( P < 0. 05 ) , compared with blank group, were nonstatistically significant ( P > 0. 05 ) . Combined drug improved intestinal motor function of diarrhea mice caused by E. coli infection. Compared with model group and blank group, small intestinal propulsion rate had significant statistical significance ( P < 0. 01) , Compared with levofloxacin group and Xiaochaihu granule group, there were statistical significance ( P < 0. 05) . Conclusion The combination of Chinese and Western medicine have a synergistic effect, improve the antibacterial and anti-inflammatory effect, reduce the use of antibiotics, at the same time, it can play the immunity-enhancing effect of Xiaochaihu KeLi, improve the body’ s resistance to foreign evil, improve the survival rate. The result of this study provide experimental basis for the clinical combination of bacterial diarrhea.

Key words:

Key words:levofloxacin, Xiaochaihu KeLi, Escherichia coli

摘要：

摘要:目的 探索研究左氧氟沙星联合小柴胡颗粒对大肠杆菌 ( E. coli) 腹泻小鼠的抗菌止泻效果。 方法 将70 只 KM 小鼠【体质量为(20±2) g,雌雄各半】随机分为 7 组,分别腹腔注射不同浓度的大肠杆菌细菌溶液,测定小鼠最小致死量( minimum lethal dose, MLD)的菌悬液浓度。 再将 50 只 KM 小鼠【体质量为(20±2) g,雌雄各半】随机分为模型组、空白组、左氧氟沙星组、小柴胡颗粒组和联合用药组(左氧氟沙星联合小柴胡颗粒)等 5 组进行体内抗菌试验,通过腹腔注射 E. coli 菌悬液( MLD 浓度)建立小鼠细菌感染腹泻模型,灌胃给予相应的药物 15 d 后,探索联合用药对 E. coli 感染致死小鼠的保护作用;通过灌胃给予相应药物 7 d 后,探索联合用药对 E. coli 感染致腹泻小鼠的腹泻指数和小鼠脏器的影响,及对小鼠小肠推进率的影响。 结果 联合用药对 E. coli 感染致腹泻的小鼠具有很好的保护作用,存活率为 90% ,与左氧氟沙星组和小柴胡颗粒组比较,联合用药组均有统计学意义( P<0. 05) ;联合用药组可以明显改善小鼠腹泻症状,与模型组和小柴胡颗粒组比较,联合用药组腹泻指数均有显著统计学意义( P<0. 01) ,与左氧氟沙星组比较,联合用药组腹泻指数有统计学意义( P< 0. 05) ;联合用药组可以对小鼠脏器具有保护作用,减轻大肠杆菌感染致腹泻对小鼠脏器毒性的影响,且可提高免疫脏器系数,提高机体免疫力,与模型组、空白组、左氧氟沙星组和小柴胡颗粒组比较,联合用药组的胸腺脏器系数和脾脏脏器系数均有统计学意义( P<0. 05) ,与模型组和左氧氟沙星组比较,联合用药组肝脏脏器系数和肾脏脏器系数均有统计学意义( P<0. 05) ,与空白组比较,均无统计学意义( P>0. 05) ;联合用药可以改善 E. coli 感染致腹泻小鼠的肠道运动功能,与模型组和空白组比较,小肠推进率有显著统计学意义( P < 0. 01) ,与左氧氟沙星组和小柴胡颗粒组比较,均有统计学意义 ( P <0. 05) 。 结论 中西药联合用药可协同作用,提高抗菌、抗炎作用,减少抗生素的使用,同时可发挥小柴胡颗粒的增强免疫力等药效,提高机体对外来邪气的抵抗能力,提高生存率,本研究将为细菌性腹泻的临床联合用药提供试验依据。

关键词:

关键词: 左氧氟沙星, 小柴胡颗粒, 大肠杆菌