Abstract: Abstract:Objective To study when and how much to dose KM mice while creating the cleft palate model induced by ＲA ( retinoic acid) ． Method Divide randomly sixty KM mice which have been identified in pregnancy into four groups (A，B，C，and D)，there were fifteen ones in each group． The mice of group A，B，C were tube-fed ＲA( retinoic acid) with 20 mg / kg、50 mg / kg and 80 mg / kg respectively at the time of GD10( gestation day 10)， while the ones of group D tube-fed with 0. 20 mL corn oil at the same time． All the mice were allowed to reproduce naturally and then record the mortality of pregnant mice，stillbirth rate and incidence of cleft palate in the live fetuses． Data were compared between groups with χ2 test using SPSS16. 0 software package． Ｒesult The incidence of live fetuses with cleft palate in group B was 93. 59%，in group C which was 20. 00%，there was significant difference in the two groups (P ＜ 0. 007)，but the stillbirth rate and mortality of pregnant mice in group C were 40% and 76. 19%，which were higher than those in group B，the differences were statistically significant ( P ＜ 0. 007) ． In addition，the pregnant mice in remaining group A and D were without the occurrence of death and the fetal mice of these groups neither appear stillbirth nor cleft palate deformity． Conclusion Tube-feeding the KM mice with ＲA 50 mg / kg at GD 10 is an appropriate  method of setting up cleft palate model

Key words: ＲA, KM mice, cleft palate model

摘要： 摘要:目的研究维甲酸( retinoic acid ＲA)诱导建立昆明小鼠腭裂模型的适宜给药时间和剂量。方法将明确孕 期的60 只昆明小鼠随机分为4 组(A、B、C、D)，每组15 只。在妊娠( gestation day，GD)10 d 时对A、B、C 组小鼠分 别管饲维甲酸20 mg / kg、50 mg / kg 和80 mg / kg，D 组管饲玉米油0. 20 mL，所有小鼠均任其妊娠足日生产，记录各组 孕鼠死亡率、死胎率及活胎鼠中腭裂发生率。采用SPSS 16. 0 软件包，用χ2 检验分析各组间差异。结果B 组活胎 鼠中腭裂发生率为93. 59% ，C 组活胎鼠中腭裂发生率为20. 00% ，二者比较差异有统计学意义(P ＜ 0. 007) ;C 组孕 鼠死亡率及死胎率分别为40. 00% 和76. 19% ，均明显高于B 组，差异有统计学意义( P ＜ 0. 007) ;A 组与D 组中孕 鼠未出现死亡，所产胎鼠无死胎亦无腭裂发生。结论给予GD 10 d 昆明小鼠管饲50 mg / kg 维甲酸是建立腭裂模 型的合适方法。

关键词: 维甲酸, 昆明小鼠, 腭裂模型