laboratory animal science ›› 2011, Vol. 28 ›› Issue (03): 42-46.

• Article • Previous Articles     Next Articles

Electrocardiogram of Healthy Rhesus Monkeys

  

  • Online:2011-06-30 Published:2013-06-04

中国恒河猴MHC I型部分等位基因的分析

  

  1. (1.广东省昆虫研究所,广州510260)(2.广东蓝岛生物技术有限公司,广州510555)
  • 基金资助:

    广州市重大科技专项(穗科条[2008]4-01);广州市重大科技专项(No:2009AI—E051);广东省科技计翅项目(No:
    20108060500023)

Abstract: Objective To investigate the electrocardiogram(ECG)features of rhesus monkeys in consciousness. Methods Sixty healthy rhesus monkeys,2—3 years old,2~3.5 kg,and 30 each gender,were selected.ECG changes of the lead I,Ⅱ,11I,aVR,aVL,aVF in resting conscious monkeys were examined,and the average heaM rate,mean electrical axis,P wave,QRS complex,amplitude of T wave,PR interval,QT interval and so on,were calculated.Results and Conclusions Sinus rhythm was found in all the 60 rhesu8 monkeys.In most of the rhesus monkeys,the P wave was upright in H lead,S wave was absent in 1I、Ul、aVF leads,and there was no Q wave in aVR lead.The average voltage of R wave in female rhesus monkeys was significantly higher than that of the males (P<0.005).In rhesus monkeys,the P wave is sharp,the QRS is diversified,the QT interval K value is 0.298± 0.030,the ST segment locates at the isoelectric line,and the T wave is smooth in six leads and upright in the II lead.In conclusion,we established the basic ECG data of rhesus monkeys.

Key words: Rhesus monkeys, Electrocardiogram(ECG)

摘要: 目的对中国恒河猴主要组织相容性复合体(MHC)l型部分基因进行携带情况调查与分析。方法采用序 列特异性引物(PCR—SSP)分型方法对华南灵长类动物研究中心繁殖的30只谱系清晰的中国恒河猴(Macaca mulatta)的32个MHC I型分子位点进行检测。结果采用的32对引物中,中国恒河猴可检出携带23个MHC—I等 位基因,但基因携带频率存在很大的差异,由3.57%至82.14%不等。结合遗传谱系分析,判断A1·21和b.2睾05 之间以及B·04和B·30之间可能就是连锁的。结论中国恒河猴携带能控制病毒复制的MHC I型基因位点的 频率较高,其基因携带频率与已发表的印度恒河猴携带频率存在明显差异。本研究为促进中国恒河猴在AIDS研 究中的应用,以及为建立携带特定MHC I基因实验猴小种群提供了依据。

关键词: MHC I型, 中国恒河猴, PCR-SSP