Abstract: To investigate the effect of nicorandil on reendothelialization of injured artery from rat carotid artery balloon-injured model. Method A total of 18 Sprague-Dawley ( SD ) rats were randomly divided into 3 groups: sham operation group, control group and experimental group, with 6 rats in each group. The common carotid artery was deendothelialised in the control group and the experimental group, while only the common carotid artery was isolated in the sham operation group. After operation, the control group was given 0. 9% sodium chloride solution ( 0. 5 mL / 100 g, once a day ) , the experimental group and sham operation group were given nicorandil ( 2 mg / 100 g, once a day ) by intragastric administration for 14 days, and common carotid artery samples were collected. The lesion area and reendothelialisation of the vascular intima were observed by Ivans’ blue staining, and the intima hyperplasia and lumen stenosis were observed by hematoxylin-eosin ( HE) staining. Result In the sham operation group, the vascular intima was smooth, the endothelium was intact, and there was no hyperplasia in the vascular intima. After 14 days of balloon injury, endothelial hyperplasia was obvious in control group, and the area of neointimal was significantly higher than that in sham operation group, the difference was statistically significant (P<0. 05) . After 14 days of balloon injury, the area of neointimal in the experimental group was significantly lower than that in the control group, and the proportion of reendothelialization area in the injured arteries was significantly higher than that in the control group, with statistical significance ( P < 0. 05 ) . Conclusion The effects by which nicorandil can facilitate reendothelialization and attenuate intimal hyperplasia of animal model of balloon injury in rat carotid artery are marked

Key words: reendothelialisation, Nicorandil, neointimal hyperplasia

摘要： 研究尼可地尔对大鼠颈总动脉球囊拉伤模型损伤血管再内皮化的影响。 方法 将 18 只 SpragueDawley( SD)大鼠随机分为 3 组,分别为假手术组、对照组、实验组,每组 6 只,对照组和实验组行颈总动脉球囊拉 伤,假手术组仅分离颈总动脉,术后对照组给予 0. 9%氯化钠溶液( 0. 5 mL / 100 g) ,每日 1 次;实验组和假手术组给 予尼可地尔(2 mg / 100 g)灌胃,每日 1 次,连续 14 d,取大鼠颈总动脉血管标本。 伊文氏蓝染色观察各组血管内膜 损伤面积及血管再内皮化的情况,HE 染色观察各组血管内膜增生及管腔狭窄程度。 结果 假手术组血管内膜光 滑,内皮层完整,血管内膜未见增生。 对照组球囊损伤 14 d 后内膜增生明显,新生内膜面积显著高于假手术组,差 异具有统计学意义(P<0. 05) 。 实验组球囊损伤 14 d 后新生内膜面积显著低于对照组,损伤血管再内皮化区域占 比显著高于对照组,差异具有统计学意义(P< 0. 05) 。 结论 尼可地尔可能抑制大鼠颈总动脉球囊拉伤后的内膜 增生,促进损伤血管再内皮化。 

关键词: 再内皮化, 尼可地尔, 新生内膜增生