关键词:  , 浅 II 度烧烫伤, 细菌感染, 皮肤全切, 损伤, 愈合

Abstract: Objective To investigate the effects of different method of modeling on skin injury and thepathological evaluation of wound healing in different time periods. Method Seventy-eight SD rats, halfmale and half female, were divided into normal control group ( 14 rats) , burn model group ( 24 rats) ,scald model group (24 rats) and total skin resection group (16 rats) . In the scald model group, the skintissue was scalded by desktop super temperature control scald instrument, and the mixed bacteria weredripped into the wound surface to establish the model of superficial II degree scald combined with woundinfection. Burn model group was established by using self-made burn conical flask to burn skin tissue anddripping mixed bacteria into the wound surface to establish the model of superficial II degree burncombined with wound infection. In the total skin resection model group, the skin was resected with asterile scalpel in an area of 3 cm × 3 cm without muscle injury to establish the total skin resection model.第 3 期 肖 洒等:不同造模方法对皮肤损伤及愈合情况的病理学评价Two animals of the normal control group were dissected and the samples were taken on the day 1, 3, 4,5, 7, 10 and 14 respectively. Four animals of the burn and scald + bacterial infection model group weredissected and the samples were taken on the day 1, 3, 5, 7, 10 and 14 respectively. Four animals of thetotal skin resection model were dissected respectively on the day 4, 7, 10 and 14. The skin tissues of thewound were examined pathologically. Result The gross observation showed that the skin of differentmodels had different degrees of wound damage at the initial stage of modeling; in the middle stage, localreactions such as redness, swelling and blisters appeared around the skin injury site; the final skinhealing is relatively complete. Microscopic observation showed that the skin of each model in juniormiddle school was mainly characterized by epidermal degeneration and necrosis, incomplete keratosis,lack of granular layer, acanthocyte layer and basal cell layer, and different degrees of inflammatory cellinfiltration in the dermis. At the end of the period, the structure of each layer of skin epidermis isrelatively complete, and a large number of new blood vessels, fibroblasts and a small amount ofinflammatory cells can be seen in the dermis. Conclusion Through the pathological evaluation of skintissue at different stages of modeling, the injury and healing of skin caused by different modeling methodwere comprehensively analyzed, which laid the foundation for the follow-up pharmacodynamic study.

Key words: superficial second degree burns and scalds, bacterial infection, total skin resection, wound;healing