关键词:  , PCPA, 失眠模型, 行为学评价

Abstract: By observing the changes in learning and memory abilities in various behavioralexperiments of the para-chlorophenylalanine ( PCPA) insomnia rat model within a week after successfulmodeling, the stability of the PCPA insomnia model was verified. Method Thirty SD rats were randomlydivided into blank group and model group, the rats in the blank group were fed normally, andestablishment of insomnia model by intraperitoneal injection of PCPA into model group rats. Fivebehavioral tests including Morris water maze test were performed in the first week after modeling.Result Compared with the blank group, the sleep latency of the model group was significantly prolonged(P < 0. 01 ) , and the sleep duration was significantly shortened ( P < 0. 01 ) . In the water mazeexperiment, the time of seeking platform in the model group was prolonged ( P < 0. 01) . In the Barnesmaze experiment, the total residence time at the target portal of the model group rats during the testexploration period was significantly shortened (P<0. 01) . In the dark avoidance experiment, the numberof errors in the model group increased ( P < 0. 05) , and the latency was significantly prolonged ( P <0. 01) . In the elevated cross maze experiment, the open arm residence time of rats in the model group实验动物科学 41 卷was significantly shortened ( P < 0. 01 ) . In the open-field experiment, the dwell time, the number ofuprightness and the total distance of movement in the central area of the model group were all lower thanthose of the blank group, among which the dwell time in the central area was significantly different (P<0. 01) . Conclusion The PCPA insomnia model has high reproducibility and stability, and can be usedas a basic model for sleep and memory-related studies.

Key words: PCPA, insomnia model, behavioural evaluation