关键词:  , 氟伐他汀钠, 斑马鱼, 抗氧化体系, 肝功能, 分子对接

Abstract: Objective To study the effects of fluvastatin sodium on the antioxidant system and liver function in the liver of zebrafish. Method Using a semi-static exposure method, zebrafish were selected as indicator organisms and exposed to blank control group and 0. 004, 0. 04 and 0. 4 mg / L of fluvastatin sodium solution, and samples were taken at 48 h after exposure to detect the effects of fluvastatin sodium on ROS content and activities of antioxidant enzymes SOD, CAT, GPx and GST in zebrafish liver. The activities of liver function indexes ALT and AST were also measured. Result Compared with the control group, the medium and high concentrations of fluvastatin sodium caused a significant increase in ROS content compared with the control group after 48 h exposure ( P < 0. 01 ) , and the activities of CAT, SOD, CuZn-SOD, and GPx changed synchronously, but the low concentration of fluvastatin sodium had no significant activation or inhibition effect on all antioxidant enzymes. Medium concentrations of fluvastatin sodium had activating effects on CAT and SOD ( P < 0. 01 ) , and high concentrations of fluvastatin sodium had activating effects on all antioxidant enzymes ( P<0. 05, P<0. 01) , while high concentrations of fluvastatin sodium had significant activating effects on ALT and AST ( P < 0. 01 ) . The molecular docking result showed that fluvastatin sodium was able to dock with zebrafish CAT protein, with the optimal docking site being Met392 and docking energy of - 4. 63 kcal / mol. Conclusion With increasing drug concentrations, fluvastatin sodium was able to affect the antioxidant system and liver function in zebrafish liver. 

Key words: fluvastatin sodium, zebrafish, antioxidant system, liver function, molecular docking