关键词:  , 非人灵长类, 保定, 应激, 皮质酮, 内啡肽, 5-羟色胺

Abstract: :Objective To investigate the effects of five times in half an hour on stress hormone levels in Rhesus Macaques. Method 3 years old Rhesus Macaques, 15 males and 15 females were divided into three groups, 5 males and 5 females in each group were trained in monkey chair restraint once every two days for 30 min each time. Group 1: no restraint training group, group 2: only restraint training group, group 3: give treats and comfort training group. A total of 5 training times, all the animals in the three groups were collected blood for analysis after each training. Result Compared with group 1, CORT was significantly decreased in groups 2 and 3 after multiple adaptive training of monkey chair preservation (P<0. 05 or P<0. 01) , β-endorphin in group 3 was significantly decreased (P<0. 05 or P<0. 01) , and 5-HT was increased in group 3 at the first and fifth adaptive training ( P<0. 01) . Conclusion When a restraint with monkey chair used in Rhesus Macaques last for half an hour, the adaptive training for the monkey chair restraint can significantly reduce stress. The animals tend to get used to or adapt to the operation of the monkey chair restraint. In adaptive training, positive reinforcement training was given to reduce stress and aggression significantly, and 3-4 times of adaptive training in the monkey chair reduced stress to a constant level.

Key words: non-human primates, restraint, stress, CORT, endorphin, 5-HT