关键词: 槐定碱, 焦虑, 行为学, 作用机制

Abstract: Objective　To verify the effectiveness and scientific validity of sophoridine in anti anxiety treatment, and to explore its preliminary mechanism of action.Methods　Forty SPF grade male C57BL/ 6J mice were randomly divided into a normal group, a low-dose sophoridine group (10 mg/kg), a medium-dose group (20 mg/kg), and a high-dose group (40 mg/kg) according to their body weight. The mice were administered at a fixed time every day for 28 days. After the administration, behavioral tests were conducted to evaluate changes in mouse anxiety behavior. ELISA experiment was used to detect the levels of SOD, NGF, IL-6, TNF-α, and GSH in mouse serum. Western blot experiment detected the interaction between sophoridine and key subunits of GABAAR. Computer molecular docking analysis of the interaction sites between sophoridine and GABAAR ultimately demonstrated the anti-anxiety efficacy and anti anxiety targets of sophoridine. Results　After intervention with sophoridine, the time and frequency of entry into the central area of OFT, the time and frequency of entry into the open arm of EPM, and the time and frequency of entry into the bright area of LDB all significantly increased. The incubation period of NSFT feeding was significantly shortened. The levels of SOD and NGF in mouse serum significantly increased. The levels of serum IL-6, TNF-α, and GSH were significantly reduced. The expression levels of GABAAR α1, β1, and γ2 subunit protein molecules were significantly increased; sophoridine alkaloid directly binds to the beta1, alpha1, and gamma2 subunits of GABAAR. Conclusion　After intraperitoneal administration of sophoridine, a potential trend toward ameliorating anxiety-like behaviors was observed, and its effects may be related to the targeting of GABAAR subunits by sophoridine. 

Key words: sophoridine, anxiety, behavior, mechanism of action