关键词: 三溴乙醇, 啮齿类动物, 麻醉, 文献计量, 动物福利

Abstract: Objective　To systematically analyze the application trends of tribromoethanol (TBE) in rodent experiments from 2020 to 2025, and to elucidate the scientific rationale and operational requirements for its standardized use, in conjunction with animal ethics review policies.Methods　A bibliometric analysis was performed based on literature retrieved from PubMed and Google Scholar. In addition, Institutional Animal Care and Use Committee (IACUC) policy documents from several foreign institutions were reviewed to summarize TBE’s pharmacological and toxicological properties, approval criteria, and operational guidelines.Results　A total of 279 articles were included. The use of TBE showed an initial increase, peaking in 2023 (63 articles), followed by a significant decline after 2024. The literature was mainly published in journals such as PLoS One and Scientific Reports, covering research areas including genetically modified animals, toxicology, and immunology. As TBE is a non pharmaceutical-grade agent with risks of hepatorenal toxicity and intestinal complications, its use requires scientific justification and strict adherence to protocols including aseptic preparation, light-protected storage, single-use administration, and combined postoperative analgesia.Conclusion　Although TBE can be used for specific short-term surgical procedures, its application is decreasing due to the rising global standards for animal welfare and experimental standardization. In practice, its use must be based on sufficient scientific rationale and strict safety protocols to balance research needs with animal ethics.

Key words: tribromoethanol, rodents, anesthesia, bibliometrics, animal welfare