Laboratory Animal Science

Analysis of Random Testing Results of Laboratory Animals in Beijing Area from 2009 to 2013

2014, 31(02): 1-6.

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Abstract:Objective To provide reference for production, management and detection of laboratory animal by analyzing of the recently 5 years' test results(2009 - 2013) on microbes, parasites and genetics of laboratory animals in Beijing area. Method According to national and local standard and relevant law and regulation, Beijing Administration Office of Laboratory Animal delegates testing agency to give random tests for qualified laboratory animal manufacturers. Testing reports could be provided by testing agency. And according to the 5 - year data from the reports, we can analyze the quality of different kinds of laboratory animal (including mice, rats, guineas, rabbits, dogs, monkeys and miniature pigs) in Beijing area. Result The quantity and batches of random testing laboratory increased by years, while the qualified rate of batches turned to be declined. Laboratory animals of different grades (SPF, CL, CV) had varying degrees infection, and toxoplasma infection existed in all the 5 years. Immune antibody non-compliance of CV laboratory animal continued to be there, and there are tendency of going up and co-infection. Meanwhile, there were 3 batches of inbred mice failing to pass the genetic monitoring. Conclusion Quality monitoring of laboratory animal is the effective means of animal quality. And it is essential to the development of medicine, life science and technology, and laboratory animal industry.

Establishment of Vill Transgenic Mouse

2014, 31(02): 7-12.

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Abstract：In order to generate Vill transgenic mouse for the functional study of Vill gene, the 2 605bp total length of Vill gene was amplified by RT-PCR and cloned into the pMD19-T vector to construct pMD19-T-Vill vector at first. The target Vill fragment with proper restriction endonuclease sites was obtained by PCR amplification using specially designed primers and pMD19-T-Vill template. This target Vill fragment and pEF6/ V5-His vector were digested respectively by SpeI and BstBI in the same time,and then were connected together to construct pEF6/ V5-His-Vill expression vector. After target gene was verified to express in L929 cells, 4.5 kb length of transgenic construction including hEF-1α promoter、Plcd1、V5、His-tag and BGH was isolated and microinjected into 390 zygotes to produce77 offspring. Finally,16 hereditable lines were established from 19 positive transgenic founder mice. General appearance was not found difference between Vill-transgenic mice and the wild type mice. The Vill transgenic mouse is potential material for the study of Vill gene functions.

Study on Identification Method for Pseudomonas aeruginosa in Laboratory Animals

2014, 31(02): 13-15.

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Abstract:Objective To study and establish accurate and rapid identification methods of Pseudomonas aeruginosa in laboratory animals. Method VITEK2 Compact automatic microbiology system and conventional biochemical methods were used to identify Pseudomonas aeruginosa reference strain, Pseudomonas aeruginosa and Pseudomonas putida isolate strains. The identification results of 4 differential media were also evaluated. Result For conventional identification methods, PIA or CFC agar media and acetamide agar media can be used as differential media, and some biochemical tests such as O/ F glucose, oxidase, 42℃ growth test were essential for the identification of Pseudomonas aeruginosa. VITEK2 Compact automated microbiology system enables the differentiation of Pseudomonas aeruginosa from similar bacteria and can serve as additional confirmatory method in conventional biochemical tests. Conclusion VITEK2 Compact automated microbiology system is able to identify Pseudomonas aeruginosa in laboratory animals accurately and efficiently.

Application of VITEK2 Compact Automatic Microbiology System in Microbiologic Testing for Laboratory Rodents

2014, 31(02): 16-19.

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Abstract:Objective To study the identification accuracy of VITEK2 Compact automatic microbiology system for pathogenic bacteria of laboratory rodents. Method VITEK2 automatic microbiology system and conventional biochemical identification methods were used to identify 16 reference strains and 148 suspect isolates. The differences on the identification accuracy and efficiency of the two methods were also analyzed. Result Comparing to the conventional identification method, VITEK2 Compact microbiology system is more accurate, efficient and capable of identifying more pathogenic bacteria. Conclusion VITEK2 Compact automatic microbiology system can be widely used in laboratory animal microbiological testing and diagnosis.

Testing of Multiple Viral Antibodies in Laboratory Rats and Mice

2014, 31(02): 20-24.

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Abstract:Objective To evaluate serologic prevalence of viral antibody in laboratory rats and mice and provide reference for the animal health monitoring and standardization of laboratory rodents. Method Samples from rats and mice submitted in 2012 and 2013 from 36 institutions were tested by enzyme-linked immune sorbent assay (ELISA), and positive samples were confirmed by immune fluorescence assay (IFA). Result Nine of 19 antibodies were positive from mouse samples and the most prevalent viruses are mouse hepatitis virus and mouse norovirus, with a prevalence of 20.44% and 19.28%, respectively. Eight of 14 antibodies were positive from rat samples and 5 items of the parvoviridae appeared to be the most prevalent viruses, with a positive ratio from 19.39% to 42.62%. Conclusion The virus monitoring and quality control for mice and rats should be strengthened.

Preliminary Study of Body Fluid Composition and Fat Distribution by Bio-impedance Spectroscopy (BIS) in Wistar Rats

2014, 31(02): 25-28.

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Abstract:Objective To study the measurement of body fluids composition and fat distribution in different gender Wistar rats by Applying BioImp Vet Body Composition. Method Body fluids components of Wistar rats including total body fluid (TBW), intracellular fluid (ICF), extracellular fluid (ECF), fat mass (FM), fatˉfree mass (FFM),TBW/ BW, ECF/ BW, ICF/ BW, FM/ BW, FFM/ BW. According to gender, normal adult Wistar rats were divided into two groups, male group (n = 13), female group (n = 13) to detect. Result There were significant differences between the male Wistar rats group and the female group. The body fluids of male Wistar rats were higher than the female. Compared FM / BW, FFM / BW of the two groups, the difference was significant (P <0.05). The percentage of fat content in the female Wistar rats was higher than the male. Conclusion The body fluids composition of the normal adult male Wistar rats was higher than the female. The percentage of fat content in the female Wistar rats was higher than the male.

Establishment of Neuropathic Pain Model by Ligated of L5 and L6 Spinal Nerve in Rats

2014, 31(02): 29-31.

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Abstract:Objective To establish the animal model of L5 and L6 spinal nerve ligation (SNL) model of neuropathic pain rats. Method According to rat pain threshold, choose 90 SD rates of male surgery operation that the left L5 and L6 spinal nerves were exposed and tightly ligated with 6 -0 black silk, postoperative measured every other day after the left hind legs of rats mechanical pain threshold, compare the preoperative and postoperative reaction to pain stimulation change in rats. Result Postoperative the left hind legs of rats pain threshold gradually reduce, to 10 days to stabilize, and the preoperative have obvious difference (P≤0.01), The right hind legs of rats pain threshold were slightly elevated, remain in a stable level. Conclusion This research established SNL rats model of mechanical pain threshold changes significantly, good stability, high reliability, can be used as a kind of neuropathy pain model in rats.

Influence of Different Sampling Methods on Blood Routine in Wistar Rat

2014, 31(02): 32-34.

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Abstract:Objective To compare the hematological indexes of blood samples collected from different ways in Wistar rats. Method Blood samples were collected from tail, orbital and abdominal aorta of 40 SPF Wistar rats and were detected by Nihon Kohden MEK - 6318K hematology analyzer. The parameters of WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, LYM, MID and GRN from three sampling ways were analyzed and pairwise compared using paired t test. Result WBC count in the tail blood was the most, and following was the orbital blood, and WBC count in the abdominal aorta blood was the least, which was 1/3 of that in the tail blood. There was highly significant difference between the three methods (P 0.05). However in female rats, HGB and RBC count in the tail blood were higher than those in the orbital blood (P<0.01). PLT count in the peripheral blood (the tail blood and the orbital blood) was less than that in the abdominal aorta blood with highly significant difference ( P< 0.01). Conclusion There were significant differences between the three methods, so each laboratory should set up own basic data for blood routine.

Study on Stem Cell Therapy of Radiation Skin Injury of Rats

2014, 31(02): 35-37.

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Abstract:Objective To investigate the stem cell therapy of radiation skin injury. Methods The animal were divided into three groups:stem cell group, positive drug group and control group. Bone marrow stromal stem cells were cultured in vitro and then transplanted on the surface of far advanced skin trauma of rats. Results The recovery rate of trauma of experimental group was(32.37 ±3.16) mm2 / d, and the average day of healing up was (20. 03 ±3. 95)d. All of these were faster than that of control group and the scar of experimental group was smaller than that of control group. There is a significant difference among medium group, experimental group and control group. Conclusion This finding indicates that there is a striking effect on treatment of skin trauma by transplanting stromal stem cells. This study should provide a new method to treatment of radiation skin injuries.

Analysis of Different MHC-B Haplotype Chicken Lines to the Resistance of Avian Leukosis

2014, 31(02): 38-43.

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Abstract:Objective To study the resistance difference of different MHC-B haplotype chicken lines to AL and establish finally a more resistant and susceptible inbred lines. Method The chicks of G1, G5, G6 and G8 were inoculated with standard strain RAV-1 of ALV A subgroup and 6 chicks of G8 line were randomly selected as control group. All died and remaining survival chicks at the end of experiment were necropsied to examine gross lesions. The serum antibody titers of all chicks were tested by the ELISA detection kit of ALV. The virus copies per 107 lymphocytes (virus load) of chicks were quantified by the duplex qRT-PCR assay. The resistance-related indexes of mortality, lesion scores, antibody titer and virus load were used to assess the AL resistance of all infected lines. Result The mortality of G5 was higher than other lines while G1 was the lowest. The antibody titer of G1 was higher than other lines from the 5 wpi to the end of the experiment while G5 was the lowest during this period. Furthermore, the antibody titer of G1 was statistically significantly (P <0.05) higher than G5 from 8 wpi to 11 wpi and from 15 wpi to the end. The lowest virus load was found on G1 from 7 wpi to 15 wpi and the virus load of G5 was statistically significantly (P < 0.01) higher than G1 from 9 wpi to 13 wpi. Conclusion All these data indicated that the G1 line was able to produce a stronger genetic resistance to the infection of ALV while G5 line was more susceptible. This study not only provides a powerful experiment data for establishing more resistant and susceptible inbred lines, but also provides significant evidence for studying the correlation between MHC and AL resistance.

Indoor Feeding Method for Spodoptera exigua

2014, 31(02): 44-46.

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Abstract:Spodoptera exigua took part in the group of laboratory animal as a freshman. The appropriate breeding condition, including temperature, humidity and illuminance, was established by means of continuing passage indoor. An exclusive prescription and usage of artificial food was also offered. Up till now, Spodoptera exigua passages over 100 generations indoor, and had a stable record of oviposition rate, hatch rate, survival rate of larva, pupation rate and eclosion rate. The Spodoptera exigua population grows steady and shows no degeneration sign.

Non Pathological Alopecia Causes of Experimental Mice

2014, 31(02): 47-48.

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Abstract : With the development of life science research, using frequency of mice was higher in animal experiments. Experimentsalist were very disturbing on mice unexplained depilation, it affected the results judgment. The non pathological alopecia in mice including natural and accidental induced the cause. The personnel can make scientific judgment according to the specificsituation.

Effect of Feed Hardness on Growth Performance of BALB/c Nude Mice

2014, 31(02): 49-51.

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Abstract: Objective To study the effect of grain feed hardness on the growth performance of BALB/c SPF nude Mice. Method 240 10-week-old SPF BALB/c nude mice were randomly divided into experiment GroupI,II,III,IV,V,and VI, and were Feeded by different hardness feed with 83.3N,96.0N,112.7N,132.3N,149.0N,And181.3N, respectively. The effect of feed hardness on the growth performance of BALB/c SPF Nude mice were observed. Result The Body weight of nude mice in experiment grup I,II and III increased significantly. However, as the particle hardness increase, the increase in body weight of nude mice was decrease. Conclusion The feed hardness is controlled in the 108-137N,the growth performance of BALA/c SPF nude mice is good. And the feed utilization is improved.

Detection on Blood Trace Elements of 6-8 Months Beagle Dogs

2014, 31(02): 52-53.

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Abstract: Objective Normal reference data of blood main trace element of 6-8 months Beagle Dogs in Sichuan Institute of Musk Deer Breeding were detected. It will provide basic data for medical research. Method Blood of 60 healthy Beagle dogs (30 males and 30 females) at the age of 6-8 months were collected by forelimb medial saphenous vein. Blood trace elements including zinc[Zn], iron[Fe], Manganese[Mn], magnesium[Mg], Lead[Pb], copper[Cu], cadmium[Cd], and calcium[Ca] were evaluated by TC - 3010A- type trace element analyzer. The data were analyzed using statistical analysis. Result Mean and standard deviation of normal reference data in Beagle dogs'blood trace elements including zinc[Zn], iron[Fe], magnesium[Mg], lead[Pb], copper[Cu] and calcium[Ca] were obtained.Mn and Cd were not detected in blood. Conclusion There are no significant difference of Fe, Ca and Pb in blood between males and females (P>0.05). But the index of Zn, Mg and Cu have significant difference (P<0.05). These knowledge was the first time to study blood trace element of 6-8 months Beagle dogs, and its results have a good referential value.

The Standardized Management for Animal Experiment Files Under the Condition of CNAS Recognized

2014, 31(02): 54-56.

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Abstract: Elaborated the importance of laboratory accreditation in the data file management. Author where the Animal laboratory, for example, laboratory data files in laboratory accreditation classification, collection and Collation of technical data files, archive program, as well as the archives standardized management.

SPF Laboratory Animals Application on Veterinary Vaccines Assessment

2014, 31(02): 57-58.

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Abstract: The source of SPF laboratory animals is definitely clear. SPF laboratory animals are not abletobe Detectedtheprimarypathogens.TheyplayanimportantroleinnotonlylifesciencereseaRCH biologicaldrugs but Alsoanimaldiseasespreventionandcontrolincludingveterinaryvaccinesassessment.

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