Laboratory Animal Science

Establishment of ELISA for the Detection of Antibodies to Mycoplasma pulmonis in Rats

He Zhengming Liu Zuomin Wang Chunling Sun Guang Wu Huiying Wei Li Zhang Ran National Institute for the Control of Pharmacentical and Biological Products Bcijing, 100050

1992, 9(03): 8-12.

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This paper reported the reseazch work on the establishment of ELISA used for the detection of antibodies against M pulmonis in rats. The reaction with 5μg of lysate antigen per ml and conjugate dilution of 1:16000 resulted in maximum ratio of P/N. OD_(492) value was varied with concentration of antigen and antibodies regularly. The ratio of P/N was higher than 2.1, even though serum was diluted in 1: 1280. Compared with the cultural isolation, ELISA is more sensitive in the detection of natural infection of M. pulmonis and the blocking ratio of positive sera was higher then 50％. Evidence above suggested that sensitivity, specificity, and reproducibility of ELISA system are satisfactory. M. pulmonis infcetion was found in 91％ of 130 conventional rats tested. The survey also showed the infection of M. arthritidis, Which is mixed infection with M pulmonis. No infection of these two mycoplasma was found in clear rats.

The Cultural Characteristics and Serodiagnosis of Polyoma Virus

Tu Xinming Jiang Hong Wu Xiaoxian Institute of Laboratory Animal Science, Chinese Academy of Medical Science Beijng, 100021

1992, 9(03): 13-15.

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The optimum culture conditions for polyoma virus is that the polyoma virus adheres to the mouse embryonic cells for 1.5 hours after inoculation, changing the medium on 4th day, and culturing in non-calf serum maintenanee medium for more than 8 days. There has been intercellular antigen while the hemagglutinin appeared in medium. A comparative test was carried out by hemagglutination inhibition (HI) test and immunoenzymatic assay (IEA) for antibody of polyoma virus on 10 SPE mice and 192 conventional mice The results shows that HI is more senstive than IEA and the serum positive for polyoma virus antibody in conventional mice, but negative in SPF mice.

Study on Application of ELISA Kit for the Detection of Antibody to PVM in Rat

Wu Huiying Chen Yonggang Zhang Ran Liu Zuomin National Institute for The Control of Pharmaceutical and Biological Products, Beijing 100050

1992, 9(03): 15-18.

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Based on the identification of antigenicity of PVM, a ELISA Kit, in which antigen was prepared by BHK21 cells inoculated with PVM, was establishment for detecting antibody to PVM in rats The conjugate (specific antibody labled with HRP) was used in ELISA-block test. IFA test was used for further verification of specificity of ELISA Kit. Antibody protein can be detected at a level of 2μg/ml. The results showed the high sensitivity and good reproducibility. This Kit was used in rountine monitoring. The results indicated that the infection rate was 38.2％ (55/114) in conventional rats and 0％ (0/10) in clear rats. Compared with two methods for selection of critical value by using OD value from 154 rats no significant diference was found.

Effect of Environmental Temperature on Determining Abnormal Toxicity of Streptomycin Sulfale in Mice

Liu Xiaoji He Meiying Tang Shanbo North China Pharmaceutical Corportion, Shijiazhuang, Hehei Province, 050015

1992, 9(03): 18-20.

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This study was designed to evalute effect of environmental temperature on the determining abnormal toxicity of streptomycin sulfale. The experimental results showed that resistance to the abnormal toxicity of streptomycin sulfale in mice was significantly redueed by variations of environmental temperature and the effect of the super-normal temperature of rearing room was most marked in several conditions of temperature. The results suggest that keeping normality and constancy of temperature in rearing room is essential to the consistency of determined results.

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