Laboratory Animal Science

Study of the Fortified Pelleted Diet for Breeding Guinea-pigs

Liu Yuan Liu Yongbo Wang Fang Laboratory Animal Center of Acadamy of Military Medical Sciences[

1994, 11(04): 0-0.

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Determining the requirement of crude protein and crude fiber in thematural-ingredient diet guinea- pig at first . Then determining theformule of fortified pelleted diet for the breeding guinea-pig accordingto aboverequirement .The reproductivity of the guinea-pigcolony byfeeding the fortifed peleted diet and autoclaved hay instead of green food is superior to the reproductivity of control animals . Realizing our ultimate aim of discontinu-ing the feeding of green food as a supplement to the breeding guinea-pigs .

The Design of Air Condition and Ventilation System With Controlable Grouping Lower Power Air Conditioner in Animal Laboratory

Liu Daoxing Cheng Shubin Jiang Qihui Geng Zhixian Laboratory Animal Center of Acadamy of Military Medical sciences Haidong Clean technology Compamy Beijing[

1994, 11(04): 0-0.

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The controlable system of air condition and ventilation wasbuild with grouping lower power airconditioners and ventilaters in class 2 small animal laboratory.This system can save energy,needs lower cost andnot large area of machine room.It is simple and convenient to use and maintain. According to inspection.itachived thetechnical standard which were published by goverment and Beijng Laboratory Animal AdministrationCommittee.

Construction of Super Clean Laboratory for SPF Chicken and its Assessment

Fan Shuixi Zhou Jiao Guo Zhihong Zhang Fangliang Yao Weiguang Yan Naiyi Su Fenglin Liu Youchang Lnstitute of Husbandry and Veterinary Medicine, Beijing Academy of Agricultural and Forestry Sciences[

1994, 11(04): 0-0.

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The key concept involved in the basic design is the isolation against contaminants while assuring necessary opera- tions of researchers and transport of materials required by the experimentation.On the one hand,most parts of the building are well sealed and air filters and various disinfectant measures are interposed at various points to cut off any contamitant that might get in.On the other hand,several channels are provided to insure the smooth but supervised flow of workers and materials in- volved.Most notably,filtered clean air forms nearly closed circulation in isolation room,with turnover amount lost via isolators and incubation room which are replenished from outside through entrance.This means both insurance of clean air and saving of en- ergy.Besides,a relatively higher or lower air pressure is maintained in a number of special isolators to prevent,respectively,the clean object(eg.healthy control)from being contaminated or the unclean object(eg.infected chicken) from making contamination of outside objects And our experience has proved the efficacy of effectively filter out most particles arising from within such as downs and feed debris, All the experimental materials are forced to make a one-way flow.With the wastes disposed at the end. Power supply is guaranteed by double lines and a selfdesigned DC plonum fan will be set in motion automatically in case of power outage.In addition,inner telephones are installed to insure timely communication which spares the need of personal movement the might cause contamination.Technical assessment has found that dust content.the noise level and indoor air pressures all reach 10 the standard.But more significantly,actual experiments using this superclean laboratory hasproved its real value.For example.sharp contrast was demonstrated between 100%protection in immunized chicken challenged with100 BID 50 of IBDV variants and 100%infection in unimmunized ones which were reared in different isolators in the same room under the same conditions,a fact that demonstrated perfect isolation among the isolators.

Some Characterizations and Tentative Applications of the Scid Mouse

Fan Wenping Li Guanmin Li Shaoming Guan Weihong Wang Xiuqing National Institute for the Control of Pharmaceutical and Biological Products People’s Republic of China [

1994, 11(04): 0-0.

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Scid mouse is homozygous for a recessive mutation at a locus thatinfluences the assembly of intact immunoglobulin and T cell receptorgenes. Therefore,scid mice cannot generate functional B and Tlymphocytes,and profoundly immunodeficient, and are valuable animal tostudy turnour.immunology,infections with pathogens and reproductivebiology. In the present study,we have surveied some characterization ofthe scid mice.The scid mice were raised in plastic film isolators inwhich SPF condition was kept.Average litter size of the scid mice was5.6.weaned-to-born ratio was 90%. By 4 to 8 weeks of age, reiativeweigts of the thymus and the spleen were 0.0433 ± 0.0l9l%,0. 0688 ± 0.0l37%respectively The size of the leukocytes per mm3 peripheral blood was 2000±339, In which the lymphocytes were 14～27% .The thymus wasfound to consist of a rudimentary medulla withour cortex and contained few lymphocytes. The spleen follicles were empty virtually devoid of lym- phocytes. occupied by reticular cell.Sera from scid mice have notbeen assayed for the presence of immnuoglobulin. The leaky ratio ofthisyoung scid mice was l 3.3%. By 35 and over weeks of age ,the majority of scid mice were leaky, and its ratio was 7l.4%. Our works will be of great value to breeding.management and quality control of scid mice. the tentative apptications of scid mice were made in this study too。

Detection of MuLv in Hybridoma and other cell line with Photobiotin-labeled Probe

Hezhengming Liu Zuomin WeiLi WuHuiying National Institute for the Control of Pharmaceutical and Biological Products Beijing[

1994, 11(04): 0-0.

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MuLV DNA fragment(5kb)in recombinant plasmid,Which wasdigested with restriction enzyme,was isolated by agarose gel electrophoresis and labeled with photobiotin as a probe. The probe was used to detect MuLv in hybridoma cells,purified McAb products and SP2/C cells with dot hyoridization.The result showed that the probe issensitive and specific to detect MuLv under 25 pg homologous DNA. In total of l3 hybridoma and 2 SP2/O cells detected.the positive rate was 38%and 50%respectively. There was no MuLv DNA founded inp uri- fiedMcAb products.

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