Abstract: Comparison was made on the development of 2-cell rabbit embryos cultured in vitro for 114 hours in 3 different media f A. Medium 199+bovine serum, B. Medium 199+albumin bovine V, and C. Medium 199+insulin + transferrin + glutamin. It demonstrated that embryos developed faster in media A and B than those in medium C. Percentage of 2-cell rabbit embryos developed to blastocysts in the above 3 media was 77.0%,64.8% and 7.O% respectively. Experinemts showed that the cultrue effect of group A was similar to group B and both of them were better than group C. So medium B was the good culture medium for studying the influencial factor of rabbit embryomic development.

Key words: Rabbit embryo In Vitro Culture Albumin bovine Ⅴ

摘要： 本试验对比兔2细胞胚胎在三种培养液中体外培养144h后的发育状况。三种培养液分别为：加小牛血清的A组M199培养液、加牛白蛋白5片段的B组M199培养液和加胰岛素、转铁蛋白及谷氨酰胺的C组M199培养液。从发育速度看，A组和B组比C组的胚胎发育快；从发育效果看，发育到囊胚和孵化囊胚的比例，A、B、C三组分别为77．0％、64．8％及7．0％。这两方面结果都说明A、B组培养效果接近，C组的培养效果较差。试验结果表明，B组培养液是研究影响兔胚胎发育因素的适宜培养液。

关键词: 兔胚胎, 体外培养, 牛白蛋白5片段