Abstract:

Objective This study aims to investigate the spectrum of enteric viruses in wild rhesus macaques, providing a scientific basis for the management and introduction of wild monkey populations, disease prevention, and occupational health protection for caregivers. Method This study collected 218 fresh fecal samples of wild rhesus macaques in Yunnan Province, fecal samples from rhesus macaques were collected, viral RNA was extracted, and RT-snPCR detection was performed using enterovirus universal primers 222 / 224 and AN88 / AN89, and positive samples were sequenced and analyzed to confirm the virus type. Result This rhesus monkey group had an enterovirus carrier rate of 11% ( 24 / 218) , as follows: baby monkeys had a carrier rate of 7. 5%(4 / 53) ; young monkeys had a carrier rate of 3. 5% (2 / 57) ; adult monkeys had a carrier rate of 16. 7%(9 / 54) ; and the carrier rate of the old monkey group was 16. 7%( 9 / 54) . Conclusion Genotyping of enteric viruses indicated that the virus spectrum primarily consisted of Enterovirus J, Enterovirus sp. and Enterovirus A groups. Phylogenetic analysis suggested that the virus spectrum is closely related to EVJ, EVA92, EVA119, SV19, and SV46 enteroviruses.

Key words: enterovirus, wild rhesus macaques, age, gender, phylogenetic analysis 

摘要： 目的 本研究旨在调查野生恒河猴的肠道病毒病原谱状况,为野生猴群疾病预防、引入及饲养管理人员职业健康防护提供科学依据。 方法 本研究收集云南省 218 份野生恒河猴新鲜粪便样本,提取病毒 RNA,采用肠道病毒通用引物 222 / 224、AN88 / AN89 进行巢式 PCR 检测,对阳性样本进行测序分析,以确认病毒种类。 结果 恒河猴肠道病毒携带率为 11%(24 / 218) ,其中,婴猴组携带率为 7. 5%(4 / 53) ;青少年猴组携带率为 3. 5% ( 2 / 57) ;成年猴组携带率为 16. 7%(9 / 54) ;老年猴组携带率为 16. 7%(9 / 54) 。 结论 经肠道病毒基因分型确定该肠道病毒病原谱主要为肠道病毒 J 组、肠道病毒 sp. 组和肠道病毒 A 组;经系统发育分析发现 218 只野生恒河猴肠道病毒病原谱与EVJ、EVA92、EVA119、SV19 和 SV46 等亲缘关系较近。

关键词: 肠道病毒, 野生恒河猴, 年龄, 性别, 系统发育