Differential Analysis of Gut Microbiota Between Guide Dogs and#br#
Eliminated Dogs Based on Metagenomic Sequencing#br#

doi:10.3969/j.issn.1006-6179.2024.04.006

Laboratory Animal Science ›› 2024, Vol. 41 ›› Issue (4): 36-40.DOI: 10.3969/j.issn.1006-6179.2024.04.006

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Differential Analysis of Gut Microbiota Between Guide Dogs and#br# Eliminated Dogs Based on Metagenomic Sequencing#br#

( 1. the First Affiliated Hospital of Dalian Medical University,Dalian 116044,China)
( 2. Laboratory Animal Center, Dalian Medical University, Dalian 116044,China)

Received:2023-11-21 Online:2024-08-28 Published:2024-08-28

基于宏基因测序的导盲犬与淘汰犬肠道菌群差异分析

( 1. 大连医科大学附属第一医院,大连 116044) ( 2. 大连医科大学实验动物中心,大连 116044)

通讯作者: 董建一( 1979—) ,女,硕士,高级实验师,研究方向为动物行为学,E-mail:beedream@ 126. com。王靖宇( 1964—) ,男,博士,教授,研究方向为动物行为学,E-mail: wangjingyus@ 163. com。
作者简介:张莹( 2002—) ,女,学士,研究方向为临床医学,E-mail:2580127690@ qq. com。
基金资助:
辽宁省教育厅 2022 年基本科研项目( LJKMZ20221269)

Abstract

Abstract: Differential analysis of intestinal flora function in guide dogs and eliminated dogs
was performed using macro gene sequencing. Method Fifteen dogs were sequenced ( nine guide dogs
and six eliminated dogs) and analyzed by applying gene number and intestinal flora diversity. Result
There were 25 630 genes specific to the guide dog group and 10 527 genes specific to the elimination dog
group. There were 2 266 up-regulated genes and 1 043 down-regulated genes with significant differences
(P<0. 05) . The abundance of Clostridium purpureum was higher in the guide dog group than in the
elimination dog group; in addition, the abundance of rumenococci differed between the two groups of dog
samples. Conclusion The diversity of intestinal flora of both guide dogs and eliminated dogs was
different and had an effect on dog behavior.

Key words: gut microbiota, metagenomics sequencing, microbiota analysis

摘要：

目的采用宏基因测序技术进行导盲犬和淘汰犬肠道菌群功能差异性分析。方法对 15 只犬进行测序( 导

盲犬 9 只,淘汰犬 6 只) ,应用基因数目、肠道菌群多样性等方法进行分析。结果导盲犬组特有 25 630 个基因,淘

汰犬组特有 10 527 个基因。具有显著差异的上调基因 2 266 个,下调基因 1 043 个( P< 0. 05) 。导盲犬组中,梭状

芽孢杆菌目的丰度高于淘汰犬组;此外,两组犬样本中瘤胃球菌的丰度也存在差异。结论导盲犬和淘汰犬的肠

道菌群多样性均存在差异性,对犬的行为有一定的影响。

关键词:

肠道菌群, 宏基因测序, 菌群分析

CLC Number:

Q95-3

ZHANG Ying , ZHOU Zijuan , GUO Yijun , HOUZHENG Zitan , WANG Jingyu , DONG Jianyi . Differential Analysis of Gut Microbiota Between Guide Dogs and#br# Eliminated Dogs Based on Metagenomic Sequencing#br#[J]. Laboratory Animal Science, 2024, 41(4): 36-40.

张莹周子娟郭怡君候正梓檀王靖宇董建一 .

基于宏基因测序的导盲犬与淘汰犬肠道菌群差异分析

[J]. 实验动物科学, 2024, 41(4): 36-40.

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[5]	. Analysis of the Differences of Gut Microbiota between Guide Dogs and Eliminated Dogs [J]. laboratory animal science, 2019, 36(03): 40-.

Differential Analysis of Gut Microbiota Between Guide Dogs and#br# Eliminated Dogs Based on Metagenomic Sequencing#br#

基于宏基因测序的导盲犬与淘汰犬肠道菌群差异分析

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