Abstract: Objective　To optimize existing modeling method and evaluation criteria to improve the uniformity, and reliability of experimental results in the rabbit ear acne model.Methods　Fifteen male New Zealand rabbits were randomly divided into five groups: a blank control group, 50% oleic acid group 1, 50% oleic acid group 2, 75% oleic acid group, and 100% oleic acid group. The inner surface of the rabbit ears were treated daily with the corresponding concentration of oleic acid solution for 14 consecutive days to establish the acne model. After modeling, no intervention was applied, and the recovery was observed for another 14 days. Symptoms at the modeling site were observed daily, ear thickness was measured weekly, and macroscopic scoring was performed. Histopathological examination of ear tissue samples were performed at the end of the modeling period and the recovery period. Additionally, another group of animals was treated with 75% oleic acid to establish the model, followed by the application of 0.1% adapalene gel. Symptoms at the modeling site were observed, ear thickness was measured, and histopathological examination was performed after the treatment.Results　The 75% oleic acid group showed superior macroscopic scores, ear thickness, hair follicle area, sebaceous gland diameter, and histopathological scores compared to the other oleic acid concentration groups. The 0.1% adapalene gel group demonstrated significant improvement in all skin-related indicators after treatment. Conclusion The application of 75% oleic acid to the entire ear successfully established a uniform and stable rabbit ear acne model. The improved macroscopic scoring system and quantitative histopathological evaluation criteria enabled precise and objective assessment of the acne model.

Key words: rabbit ear, acne model, oleic acid, hair follicle area, sebaceous gland diameter

摘要： 目的　对现有的建模方法和评价指标进行优化以提高兔耳痤疮模型的均一性和实验结果的可靠性。方法 雄性新西兰白兔15只,按体质量随机分为空白对照组、50%油酸1组、50%油酸2组、75%油酸组、100%油酸组。每 日于兔耳内侧面涂抹相应浓度油酸溶液1次,连续14 d制备兔耳痤疮模型,造模结束后不做任何干预恢复观察 14 d,每日观察造模部位症状,每周进行耳厚度测量及肉眼观察评分,并于末次造模后及恢复期结束时取耳片进行 组织病理学检查。另取动物同法采用75%油酸造模后,给予0.1%阿达帕林凝胶,观察造模部位症状、测量耳厚度, 给药10 d后取耳片进行组织病理学检查。结果　75%油酸造模后动物肉眼观察评分、耳厚度、毛囊面积和皮脂腺 直径及组织病理学检查评分均优于其他浓度油酸组。0.1%阿达帕林凝胶给药后动物皮肤各项指标均得到明显改 善。结论　采用75%油酸整耳涂抹可成功建立均一性好且稳定的兔耳痤疮模型,改良后的肉眼观察评分体系及量 化的组织病理学检查指标可实现对痤疮模型精准、客观的评价。

关键词: 兔耳, 痤疮模型, 油酸, 毛囊面积, 皮脂腺直径