Abstract: Objective　To explore the differential metabolites and metabolic pathways related to skin injury repair in iRhom2^Uncv, and to provide a basis for in-depth study of potential biomarkers and targets for skin injury repair in iRhom2^Uncv.Methods　Thirty-two SPF-grade adult male iRhom2^Uncv and BALB/c mice were randomly divided into four groups. A full-thickness skin wound model was established under anesthesia. The skin tissues were analyzed with immunohistochemistry at 0, 5 and 12 days post injury. The serum was collected for metabolite analysis.Results　The wound healing rate of iRhom2^Uncv was slightly higher than that of BALB/c. The inflammatory response of iRhom2^Uncv was higher, and the number of new blood vessels at 12 days post-injury was higher than that of wild-type BALB/c mice. Differential metabolites were significantly enriched in six metabolic pathways, including arachidonic acid metabolism, regulation of inflammatory mediators in TRP channels, histidine metabolism and thyroid hormone synthesis.Conclusion　The ability of iRhom2^Uncv in skin wound healing is stronger than that of BALB/c, and the affected metabolites and metabolic pathways can provide a basis for further research of the metabolic mechanism related to skin wound repair in iRhom2^Uncv.

Key words: iRhom2^Uncv, skin wound, mouse, metabolome 

摘要： 目的　探索iRhom2^Uncv小鼠皮肤损伤修复特征及其相关的差异代谢物和代谢通路,为深入研究iRhom2^Uncv小 鼠皮肤损伤修复的潜在生物标志物和靶点提供依据。方法　选取9~10周龄的SPF级iRhom2Uncv小鼠和野生型 BALB/c小鼠,各32只,随机分成0 d组、5 d组、12 d组和16 d组,每组8只,麻醉后于背部做直径为10 mm的圆形 全皮层创口,建立皮肤缺损模型。造模结束后,用CO2 麻醉后处死0 d组、5 d组和12 d组小鼠,采集皮肤组织进行 免疫组织化学染色,检测肿瘤坏死因子-α和血小板内皮细胞黏附分子-1的表达,收集其新鲜血清,用于代谢物分 析。结果　iRhom2^Uncv小鼠的愈合速度比野生型BALB/c小鼠稍快,iRhom2^Uncv小鼠的炎性反应较高,12 d新血管数量 较野生型BALB/c小鼠多。差异代谢物主要富集在花生四烯酸代谢、瞬时受体电位通道的炎症介质调节、组氨酸代谢 和甲状腺激素合成等6个代谢通路。结论　iRhom2^Uncv小鼠皮肤创伤后的愈合能力强于野生型BALB/c小鼠,受到影 响的代谢物和代谢途径可为深入研究iRhom2^Uncv小鼠皮肤损伤修复相关代谢机制提供依据。

关键词: iRhom2^Uncv, 皮肤损伤, 小鼠, 代谢组