Abstract: Objective　To systematically analyze the application trends of tribromoethanol (TBE) in rodent experiments from 2020 to 2025, and to elucidate the scientific rationale and operational requirements for its standardized use, in conjunction with animal ethics review policies.Methods　A bibliometric analysis was performed based on literature retrieved from PubMed and Google Scholar. In addition, Institutional Animal Care and Use Committee (IACUC) policy documents from several foreign institutions were reviewed to summarize TBE’s pharmacological and toxicological properties, approval criteria, and operational guidelines.Results　A total of 279 articles were included. The use of TBE showed an initial increase, peaking in 2023 (63 articles), followed by a significant decline after 2024. The literature was mainly published in journals such as PLoS One and Scientific Reports, covering research areas including genetically modified animals, toxicology, and immunology. As TBE is a non pharmaceutical-grade agent with risks of hepatorenal toxicity and intestinal complications, its use requires scientific justification and strict adherence to protocols including aseptic preparation, light-protected storage, single-use administration, and combined postoperative analgesia.Conclusion　Although TBE can be used for specific short-term surgical procedures, its application is decreasing due to the rising global standards for animal welfare and experimental standardization. In practice, its use must be based on sufficient scientific rationale and strict safety protocols to balance research needs with animal ethics.

Key words: tribromoethanol, rodents, anesthesia, bibliometrics, animal welfare

摘要： 目的　系统分析2020—2025年三溴乙醇(TBE)在啮齿类动物实验中的应用趋势,并结合动物伦理审查政策, 阐明其规范使用的科学依据与操作要求,为科研人员提供合规使用参考。方法　基于PubMed、Google Scholar等数 据库进行文献检索与计量分析,纳入相关文献279篇;同时梳理部分国外机构IACUC政策文件,总结TBE的药理 毒理特性、审批标准及操作规范。结果　TBE应用呈先增后降趋势,2023年达峰值(63篇),文献主要发表于PLoS One、Scientific Reports等期刊,集中于基因编辑动物、毒理学、免疫学等领域;2024年后发文量显著下降。TBE属非 药物级制剂,具有肝肾毒性及肠道并发症风险,其使用需提供科学必要性论证,并严格遵循无菌配制、避光储存、单 次使用及联合镇痛等规范。结论　TBE可用于特定短期手术,但受全球动物福利与实验标准化要求提升影响,其 应用正逐渐减少。实际使用中,须基于充分科学理由并严格执行安全规程,以平衡科研需求与动物伦理。

关键词: 三溴乙醇, 啮齿类动物, 麻醉, 文献计量, 动物福利