Abstract: Objective　To validate the time-dependent changes in the sterilization efficacy of 75% ethanol after opening and to determine its safe use period to ensure disinfection effectiveness.Methods　After opening, 75% ethanol was transferred into a spray bottle and placed in a laboratory animal barrier facility at room temperature. A quantitative suspension bactericidal test was performed using Staphylococcus aureus as the test strain to evaluate the bactericidal efficacy of 75% ethanol at different time intervals (0 30 d) after opening.Results　The quantitative suspension bactericidal test demonstrated no significant decline in the bactericidal efficacy of 75% ethanol against Staphylococcus aureus after 30 d of room temperature storage. The log reduction value (LRV) remained above 5.0 throughout the testing period. Conclusion　After being transferred to spray bottle and stored at room temperature, 75% ethanol shows no significant decline in its bactericidal efficacy against Staphylococcus aureus within 30 d, which can serve as evidence for extending its service life.

Key words: 75% ethanol, Staphylococcus aureus, disinfection efficacy 

摘要： 目的　验证75%乙醇开封后,其灭菌效力随时间增加的变化,确定其使用有效期,确保其消毒效力。方法 75%乙醇开封后灌注于喷壶中,于实验动物屏障设施内常温放置。以金黄色葡萄球菌为测试菌株,通过菌悬液定 量杀菌实验法检测开封后不同时间段75%乙醇(0~30 d)的杀菌效果。结果　菌悬液定量杀菌实验结果显示,75% 乙醇开封后室温存放至第30天,其对金黄色葡萄球菌的杀灭对数值仍维持在5.0以上。结论　75%乙醇开封后于 喷壶中常温放置,30 d内对金黄色葡萄球菌杀菌效力未见显著降低,可作为其延长使用期限的佐证。

关键词: 75%乙醇, 金黄色葡萄球菌, 消毒效力