Abstract: Objective　To investigate the differences in gene expression in different tissues of Sigmodon hispidus, and promote the open sharing of laboratory animal resource.Methods　RNA-seq sequencing technology was used to perform transcriptome sequenceing and bioinformatics analysis on five tissues (heart, liver, spleen, lungs, and kidney) of Sigmodon hispidus. Results　After quality control processing, a total of 461 666 539 sequences were obtained from 20 samples, with the base number ranging from 6.37 G to 7.99 G for each sample. The clean reads were assembled using Trinity software to obtain 216 139 unigenes with an average length of 1 170 bp. A total of 168 458(77.93%) unigenes were successfully annotated in seven databases, including 26 394 (12.21%) and 38 056 (17.60%) unigenes annotated in KEGG and GO databases, respectively. Through further analysis of the gene expression levels between different tissues, found that the number of uniquely expressed genes in the heart, liver, spleen, lung, and kidney were 12 297, 14 477, 35 104, 23 728, and 9 636, respectively. And a total of 103 359 differentially expressed genes between different tissues was detected.Conclusion　This study obtained gene expression data of different tissues of Sigmodon hispidus,through transcriptome sequencing analysis, providing reference for further research on the biological characteristics of Sigmodon hispidus and the development and utilization of functional gene resources of this laboratory animal.

Key words: Sigmodon hispidus, RNA-seq sequencing, gene expression, gene function annotation

摘要： 目的　探讨刚毛棉鼠不同组织间基因表达的差异,促进该实验动物资源的开放共享。方法　采用RNA-seq 测序技术对刚毛棉鼠的5种组织(心脏、肝、脾、肺和肾)进行转录组测序及生物信息学分析。结果　20个样本共 获得461 666 539条质控后的序列,各组织质控后的碱基数为6.37 G~7.99 G。测序数据通过Trinity组装得到 216 139条unigenes,平均长度为1 170 bp。采用7个数据库进行基因功能注释,得到了168 458个(77.93%)有功能 注释信息的unigenes,其中在KEGG和GO数据库中分别有26 394(12.21%)和38 056(17.60%)条注释信息。通过 对不同组织间基因表达水平进一步分析,发现心脏、肝、脾、肺、肾中唯一表达的基因数分别为12 297、14 477、 35 104、23 728和9 636个,不同组织间差异表达基因数共计103 359个。结论　通过转录组测序分析获得了刚毛 棉鼠不同组织的基因表达数据,为深入研究刚毛棉鼠的生物学特性和开发利用刚毛棉鼠的功能基因资源提供了参 考资料。

关键词: 刚毛棉鼠, 转录组测序, 基因表达, 基因功能注释