Abstract: Innate lymphoid cells (ILC), a newly identified class of innate immune cells in recent years, exhibit a broad spectrum of functions. Based on their phenotypic characteristics, ILCs can be categorized into three main subsets, ILC1s, ILC2s, and ILC3s. The development of specific animal experimental models offers significant advantages for elucidating the precise biological functions of ILCs. However, the complete and specific ablation of these cells is technically challenging due to the lack of ILC-specific promoters. To overcome this limitation, strategies targeting the upstream regulators of ILC development and functional pathways have been employed to generate animal models with ILC-specific defects. This review systematically summarizes the ILC-deficient mouse models established through diverse strategies, which are based on the distinct functional properties of ILCs, and provides an important theoretical basis for the construction of relevant models in various stages of innate lymphoid cell research.

Key words: innate lymphoid cells, mouse model, gene knockout

摘要： 先天淋巴细胞(ILC)是近年研究新发现的一组先天免疫细胞,其作用广泛,依据表型特征的差异可分为 ILC1s、ILC2s及ILC3s。在解析ILC的具体功能时,构建特异性动物实验模型的优势非常显著。由于缺乏相应特殊 引导启动子,完全单独去除这组细胞存在困难,但可以从ILC的起源及发挥功能的通路角度出发,通过“抑制”或 “敲除”其上游调控靶点,构建ILC特异性缺陷的动物模型。本文系统综述了基于ILC不同功能特性、通过多种策 略建立的ILC缺陷小鼠模型,为先天淋巴细胞各阶段研究中模型的构建提供了重要的理论依据。

关键词: 先天淋巴细胞, 小鼠模型, 基因敲除