Abstract: Objective To investigate the role of three imaging modalities including MRI, in vivo imaging and ultrasound in evaluating chemotherapy in lymphoma-bearing mice. Method 10 BALB / c female mice were subcutaneously injected with lymphoma cells on the right neck and shoulder to build a tumor-bearing model, and were randomly divided into a CTX intervention group and a 0. 9% NS intervention group. Before and after the intervention, MRI, in vivo imaging, and Ultrasound were used to detect tumor-related indicators. After the detection, the mice were sacrificed, and the tumor was taken for volume measurement and pathological examination. Result MRI accurately demonstrated a decrease in tumor size after intervention, which was comparable to gross measurements. In vivo imaging can indicate that the tumor size was reduced and the fluorescence intensity of the tumor decreased after the intervention. Combined with the analysis of the pathological section, the CEUS demonstrated that the perfusion of the tumor-related blood vessels decreased after the intervention, and the differences were statistically significant. Conclusion The three imaging modalities have the advantages of good imaging effect, high imaging sensitivity, non-invasive and reproducible, and the combination of multiple imaging modalities has a better synergistic evaluation effect on the intervention indicators of tumor mouse models.

Key words: lymphoma tumor-bearing mice, MRI, in vivo imaging, CEUS, tumor angiogenesis

摘要： 目的 探讨磁共振、可见光活体成像、超声三种成像模式在淋巴瘤荷瘤鼠化疗评估中的作用。 方法 取 10 只 BALB / c 雌性小鼠于右侧颈肩部皮下注射淋巴瘤细胞建立淋巴瘤荷瘤模型,随机分为环磷酰胺治疗组和 0. 9%氯化钠溶液对照组。 于开始干预前,干预后分别用磁共振、可见光活体成像和超声技术检测肿瘤,并将小鼠安乐死,取瘤体测量体积及病理检查。 结果 干预后磁共振精确证明肿瘤变小,与大体测量结果相仿;活体成像能够提示干预后肿瘤范围缩小,肿瘤荧光强度下降;结合病理切片分析,超声造影证明干预后肿瘤相关血管灌注下降,差距均有统计学意义。 结论 三种成像模式均有着成像效果佳,显像灵敏度高,无创可重复的优势,多成像模式结合对肿瘤小鼠模型干预有较佳的协同评估作用。

关键词: 淋巴瘤荷瘤鼠, 磁共振;活体成像;超声造影;肿瘤血管