Abstract: Abstract: Objective To establish the Alzheimer’ s disease ( AD) rats model induced by multi-factors. Method Twenty SD rats were randomly divided into the control group and the model group, with 10 rats in each group. To build the AD models, D-galactose ( 60 mg / kg) was injected intra-abdominally, meanwhile, aluminium chloride (100 mg / kg) was hypodermically injected for 45 days. The learning and memory abilities of model rats were assessed using Morris water maze test. The level of acetyl cholinesteraseinin rat brain tissue was also measured. Result In the Morris water maze test, compared with the control group, the escape latency of the rat in the model group was significantly prolonged ( P<0. 01) , through the time and distance in target area was obviously increased, the number of active explorations through the platform was significantly reduced ( P < 0. 01 ) . The acetyl cholinesterase levels in brain tissue were increased significantly ( P < 0. 01) . Conclusion A complicated AD rat model was successfully established by combined intervention of D-galactose and aluminium chloride.

Key words: Key words:Alzheimer’ s disease, rats, animal mode, aluminium chloride, D-galactose

摘要： 摘要:目的 建立阿尔茨海默病( AD)复合大鼠模型。 方法 SD 大鼠 20 只,随机分为正常组和模型组,每组 10 只。模型组大鼠每天腹腔注射 D-半乳糖 60 mg / kg,同时皮下注射三氯化铝 100 mg / kg,连续 45 d;采用 Morris 水迷宫考察其学习记忆能力,并检测大鼠脑组织乙酰胆碱酯酶的活性。 结果 水迷宫测试显示,模型组大鼠与正常组大鼠相比,潜伏期明显延长( P<0. 01) ,平台所在象限的游泳时间和距离百分比增高( P< 0. 01) ,但穿越平台次数明显减少( P<0. 01) ,脑部乙酰胆碱酯酶活力增高( P<0. 01) 。 结论 大鼠经 D-半乳糖复合三氯化铝造模建立的模型可作为 AD 动物模型。

关键词: 关键词:阿尔茨海默病, 大鼠, 动物模型, 三氯化铝, D-半乳糖