Abstract:

Abstract: Objective Experimental animal anesthesia is an important part of animal experiments. We have previously noticed that the usage of anesthetics for about 1 hour will have a certain effect on the white blood cells of mice. Therefore, this study observed the effects of three commonly used animal anesthetics on blood glucose and blood routine indicators in male mice, and observed the changes in blood routine caused by the same anesthetic over time. Method Anesthetized mice were anesthetized by intraperitoneal injection. Blood samples and blood routine values were taken from the tail vein of mice before injection, 15 min and 60 min after injection. Result Ethyl carbamate can significantly increase blood sugar in mice, while Chloraldurat and pentobarbital sodium have no significant effect on blood glucose. Ethyl carbamate and pentobarbital sodium can increase the total amount of white blood cells in mice, mainly in the neutrophils and monocytes. The effect of Chloraldurat is the decrease of the total amount of white blood cells, neutrophils and significant reduction in lymphocytes. Conclusion In blood glucose-related studies, the use of ethyl carbamate should be avoided. The use of ethyl carbamate, chloraldurat and pentobarbital sodium in the study of leukocyte-related studies requires detailed design to avoid data interference due to anesthetics.

摘要：

摘要:目的 实验动物麻醉是动物实验中一个重要的环节,我们前期关注到,使用麻醉药 1 h 左右会对小鼠的白细胞总数造成一定的影响。 因此,本研究观察了三种常用动物麻醉药物(氨基甲酸乙酯、水合氯醛和戊巴比妥钠) 对雄性小鼠血糖和血液常规指标的影响,并观察了同一麻醉药随时间延长而引起的血常规变化情况。 方法 采用腹腔注射麻醉小鼠,分别在注射前、注射后 15 min 和 60 min,从小鼠尾静脉取血测定血糖值和血常规值。 结果 氨基甲酸乙酯能明显提高小鼠血糖,水合氯醛与戊巴比妥钠对血糖无明显影响。 氨基甲酸乙酯和戊巴比妥钠可使小鼠白细胞总数增加,并主要表现在中性粒细胞数和单核细胞数的升高,水合氯醛的影响表现为白细胞总数下降,中性粒细胞数和淋巴细胞数的减少显著。 结论 在血糖相关研究中,应避免使用氨基甲酸乙酯。 在涉及白细胞相关研究过程中,氨基甲酸乙酯、水合氯醛和戊巴比妥钠的使用需进行细节性设计,以避免由于麻醉药导致的数据干扰。