Abstract: Abstract: Objective To investigate the difference of expression of nerve growth factor( NGF) at different time by rats that induced epilepsy by kainic acid ( KA) ． Method The epilepsy model was used kainic acid intraperitoneal injectioned in rat． The nerve growth factor expression were detected by real-time fluorescent quantitative PCＲ assay with TaqMan probe in different time after intraperitoneal KA． Ｒesult Compared with normal sodium( NS) group， the expression of NGF were lower at 6—12 h ，and were higher at 48 h and 72 h( P ＜ 0. 05) ，but the expression of NGF were risied up at 48—72 h and higher by NS． Conclusion NGF have reparation and protection the hippocampus of rats that induced epilepsy by KA．

Key words: <p>epilepsy, real time PCＲ, nerve growth factor, seizure activity, reparation</p>

摘要： 摘要: 目的 探讨红藻氨酸( Kainic Acid，KA) 致癫痫大鼠癫痫发作过程中海马组织神经生长因子( nerve growth factor，NGF) 不同时间点表达量的变化。方法 腹腔注射 KA 建立大鼠癫痫模型。采用 TaqMan 探针实时定量 PCＲ ( Ｒeal-time quantitative PCＲ) 技术，检测注射 KA 后不同时点大鼠海马组织中 NGF 表达量的变化。结果 与 NGF 表达量与生理盐水对照组( NS) 相比，6 ～ 12 h NGF 表达量明显低于 NS 组( P ＜ 0. 05) 、48 ～ 72 h NGF 表达量开始升高并高于 NS 组，在 24、72 h 时其表达量显著高于 NS 组( P ＜ 0. 05) 。结论 NGF 对致癫大鼠的海马具有修复与保护作用。

关键词: <, p>, 癫痫, 定量 PCＲ, 神经生长因子, 发作程度, 修复<, /p>